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Current cultivar Wild relative F1 Generation F2 Generation Back cross Many backcrosses New cultivar Beneficial gene Unwanted genes Beneficial gene Unwanted genes Unrelated organism Transfer into plasmid in Agrobacterium Current cultivar Genes required for transfer New cultivar Beneficial gene Unwanted genes Transfer into plasmid in Agrobacterium Current cultivar Genes required for transfer New cultivar Wild relative Conventional breeding Transgenesis Cisgenesis