CRISPOR (original) (raw)

CRISPOR (citation) is a program that helps design, evaluate and clone guide sequences for the CRISPR/Cas9 system. CRISPOR Manual
July 18, 2024: The old server has been retired. The new Python3 server is still lacking the Najm 2018 saCas9 score. See Full list of changes

Text case is preserved, e.g. you can mark ATGs with lowercase.
Instead of a sequence, you can paste a chromosome range, e.g. chr1:11,130,540-11,130,751

Step 3

Select a Protospacer Adjacent Motif (PAM)

See notes on enzymes in the manual.