Cohn process (original) (raw)
Die Cohn-Extraktion, synonym Cohn-Fraktionierung, ist eine Extraktion von Albuminen aus Blutplasma, die von dem US-amerikanischen Biochemiker Edwin J. Cohn (1892–1953) entwickelt wurde.
| Property | Value |
|---|---|
| dbo:abstract | Die Cohn-Extraktion, synonym Cohn-Fraktionierung, ist eine Extraktion von Albuminen aus Blutplasma, die von dem US-amerikanischen Biochemiker Edwin J. Cohn (1892–1953) entwickelt wurde. (de) The Cohn process, developed by Edwin J. Cohn, is a series of purification steps with the purpose of extracting albumin from blood plasma. The process is based on the differential solubility of albumin and other plasma proteins based on pH, ethanol concentration, temperature, ionic strength, and protein concentration. Albumin has the highest solubility and lowest isoelectric point of all the major plasma proteins. This makes it the final product to be precipitated, or separated from its solution in a solid form. Albumin was an excellent substitute for human plasma in World War Two. When administered to wounded soldiers or other patients with blood loss, it helped expand the volume of blood and led to speedier recovery. Cohn's method was gentle enough that isolated albumin protein retained its biological activity. (en) Il processo Cohn o frazionamento a freddo con etanolo, sviluppato da Edwin J. Cohn, è una procedura di frazionamento del plasma allo scopo di estrarre albumina dal plasma sanguigno. Il metodo di Cohn è così funzionale che la proteina isolata dell'albumina mantiene la sua attività biologica. Il processo si basa sulla differenza di solubilità dell'albumina e di altre proteine plasmatiche in base a pH, concentrazione di etanolo, temperatura, forza ionica e concentrazione di proteine. L'albumina ha la più alta solubilità e il punto isoelettrico più basso di tutte le principali proteine plasmatiche; questo permette al prodotto finale di separarsi dalla sua soluzione in forma solida. L'albumina è stata un eccellente sostituto del plasma umano nella seconda guerra mondiale. Quando somministrato a soldati feriti o altri pazienti con perdita di sangue, aiutava ad espandere il volume del sangue e portava a un recupero più rapido. (it) |
| dbo:wikiPageID | 8116008 (xsd:integer) |
| dbo:wikiPageLength | 15770 (xsd:nonNegativeInteger) |
| dbo:wikiPageRevisionID | 1088842057 (xsd:integer) |
| dbo:wikiPageWikiLink | dbr:Precipitation_(chemistry) dbr:Blood_plasma dbc:Blood_products dbr:Edwin_Joseph_Cohn dbr:Coprecipitation dbc:Industrial_processes dbr:Ethanol dbr:PH dbr:Globulin dbr:Isoelectric_point dbc:Fractionation dbr:Fibrin_sealant dbc:Medical_technology dbc:Transfusion_medicine dbc:Biochemical_separation_processes dbc:Blood dbr:Biological_activity dbr:Blood_plasma_fractionation dbr:Thrombin dbr:Fibrin dbr:Fibrinogen dbr:Human_serum_albumin dbr:Chromatography_in_Blood_Processing dbr:Solubility dbr:Martin_MacPhee |
| dbp:wikiPageUsesTemplate | dbt:Cn dbt:Reflist dbt:Short_description |
| dct:subject | dbc:Blood_products dbc:Industrial_processes dbc:Fractionation dbc:Medical_technology dbc:Transfusion_medicine dbc:Biochemical_separation_processes dbc:Blood |
| gold:hypernym | dbr:Series |
| rdf:type | yago:WikicatBloodProducts yago:Artifact100021939 yago:Commodity103076708 yago:Merchandise103748886 yago:Object100002684 yago:PhysicalEntity100001930 dbo:TelevisionShow yago:Whole100003553 |
| rdfs:comment | Die Cohn-Extraktion, synonym Cohn-Fraktionierung, ist eine Extraktion von Albuminen aus Blutplasma, die von dem US-amerikanischen Biochemiker Edwin J. Cohn (1892–1953) entwickelt wurde. (de) The Cohn process, developed by Edwin J. Cohn, is a series of purification steps with the purpose of extracting albumin from blood plasma. The process is based on the differential solubility of albumin and other plasma proteins based on pH, ethanol concentration, temperature, ionic strength, and protein concentration. Albumin has the highest solubility and lowest isoelectric point of all the major plasma proteins. This makes it the final product to be precipitated, or separated from its solution in a solid form. Albumin was an excellent substitute for human plasma in World War Two. When administered to wounded soldiers or other patients with blood loss, it helped expand the volume of blood and led to speedier recovery. Cohn's method was gentle enough that isolated albumin protein retained i (en) Il processo Cohn o frazionamento a freddo con etanolo, sviluppato da Edwin J. Cohn, è una procedura di frazionamento del plasma allo scopo di estrarre albumina dal plasma sanguigno. Il metodo di Cohn è così funzionale che la proteina isolata dell'albumina mantiene la sua attività biologica. L'albumina è stata un eccellente sostituto del plasma umano nella seconda guerra mondiale. Quando somministrato a soldati feriti o altri pazienti con perdita di sangue, aiutava ad espandere il volume del sangue e portava a un recupero più rapido. (it) |
| rdfs:label | Cohn-Extraktion (de) Cohn process (en) Processo di Cohn (it) |
| owl:sameAs | freebase:Cohn process yago-res:Cohn process wikidata:Cohn process dbpedia-de:Cohn process dbpedia-it:Cohn process https://global.dbpedia.org/id/4hpeX |
| prov:wasDerivedFrom | wikipedia-en:Cohn_process?oldid=1088842057&ns=0 |
| foaf:isPrimaryTopicOf | wikipedia-en:Cohn_process |
| is dbo:wikiPageRedirects of | dbr:Cohn_Process dbr:Cohn's_fractionation dbr:Cohn_cold_ethanol_fractionation dbr:Cohn_method |
| is dbo:wikiPageWikiLink of | dbr:Antiserum dbr:Rho(D)_immune_globulin dbr:Von_Willebrand_disease dbr:Bovine_serum_albumin dbr:Snake_venom dbr:Cohn_Process dbr:Albumin dbr:Chromatography_in_blood_processing dbr:Protein_precipitation dbr:Hepatitis_B_immune_globulin dbr:Blood_plasma_fractionation dbr:Cohn's_fractionation dbr:Cohn_cold_ethanol_fractionation dbr:Cohn_method |
| is rdfs:seeAlso of | dbr:Blood_fractionation |
| is foaf:primaryTopic of | wikipedia-en:Cohn_process |