DsrA RNA (original) (raw)
DsrA RNA is a non-coding RNA that regulates both transcription, by overcoming transcriptional silencing by the nucleoid-associated H-NS protein, and translation, by promoting efficient translation of the stress sigma factor, RpoS. These two activities of DsrA can be separated by mutation: the first of three stem-loops of the 85 nucleotide RNA is necessary for RpoS translation but not for anti-H-NS action, while the second stem-loop is essential for antisilencing and less critical for RpoS translation. The third stem-loop, which behaves as a transcription terminator, can be substituted by the trp transcription terminator without loss of either DsrA function. The sequence of the first stem-loop of DsrA is complementary with the upstream leader portion of RpoS messenger RNA, suggesting that p
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| dbo:abstract | DsrA RNA is a non-coding RNA that regulates both transcription, by overcoming transcriptional silencing by the nucleoid-associated H-NS protein, and translation, by promoting efficient translation of the stress sigma factor, RpoS. These two activities of DsrA can be separated by mutation: the first of three stem-loops of the 85 nucleotide RNA is necessary for RpoS translation but not for anti-H-NS action, while the second stem-loop is essential for antisilencing and less critical for RpoS translation. The third stem-loop, which behaves as a transcription terminator, can be substituted by the trp transcription terminator without loss of either DsrA function. The sequence of the first stem-loop of DsrA is complementary with the upstream leader portion of RpoS messenger RNA, suggesting that pairing of DsrA with the RpoS message might be important for translational regulation. The structures of DsrA and DsrA/rpoS complex were studied by NMR. The study concluded that the sRNA contains a dynamic conformational equilibrium for its second stem–loop which might be an important mechanism for DsrA to regulate the translations of its multiple target mRNAs. There is evidence that DsrA RNA can self-assemble into nanostructures through antisense interactions of three self-complementary regions. (en) |
| dbo:thumbnail | wiki-commons:Special:FilePath/RF00014.jpg?width=300 |
| dbo:wikiPageExternalLink | http://www.rnajournal.org/cgi/pmidlookup%3Fview=long&pmid=18658123 https://web.archive.org/web/20110707013004/http:/ccb.bmi.ac.cn/srnatarbase/details.php%3Fid=1 https://web.archive.org/web/20110707013047/http:/ccb.bmi.ac.cn/srnatarbase/details.php%3Fid=2 https://web.archive.org/web/20110707013108/http:/ccb.bmi.ac.cn/srnatarbase/details.php%3Fid=3 https://web.archive.org/web/20110707013113/http:/ccb.bmi.ac.cn/srnatarbase/details.php%3Fid=4 https://web.archive.org/web/20110707013118/http:/ccb.bmi.ac.cn/srnatarbase/details.php%3Fid=6 |
| dbo:wikiPageID | 11420785 (xsd:integer) |
| dbo:wikiPageLength | 22793 (xsd:nonNegativeInteger) |
| dbo:wikiPageRevisionID | 1079708359 (xsd:integer) |
| dbo:wikiPageWikiLink | dbr:Nucleotide dbr:Arginine_repressor_ArgR dbr:Nucleoid dbr:Gene dbr:Bacteria dbc:Non-coding_RNA dbr:Transcription_(genetics) dbr:Tryptophan dbr:Nucleotides dbr:RNA dbr:Bacterial_small_RNA dbr:Stem-loop dbr:Translation_(biology) dbr:Cis-natural_antisense_transcript dbr:Sequence_conservation dbr:Nuclear_magnetic_resonance dbr:RpoS dbr:Non-coding_RNA dbr:Secondary_structure dbr:Hfq |
| dbp:caption | Predicted secondary structure and sequence conservation of DsrA (en) |
| dbp:id | RF00014 (en) |
| dbp:name | DsrA RNA (en) |
| dbp:rfam | RF00014 (en) |
| dbp:rnaType | dbr:Gene dbr:Bacterial_small_RNA |
| dbp:symbol | DsrA (en) |
| dbp:taxDomain | dbr:Bacteria |
| dbp:wikiPageUsesTemplate | dbt:Cite_journal dbt:Reflist dbt:Short_description dbt:SO dbt:Infobox_rfam dbt:Rfam |
| dcterms:subject | dbc:Non-coding_RNA |
| gold:hypernym | dbr:RNA |
| rdf:type | dbo:Protein |
| rdfs:comment | DsrA RNA is a non-coding RNA that regulates both transcription, by overcoming transcriptional silencing by the nucleoid-associated H-NS protein, and translation, by promoting efficient translation of the stress sigma factor, RpoS. These two activities of DsrA can be separated by mutation: the first of three stem-loops of the 85 nucleotide RNA is necessary for RpoS translation but not for anti-H-NS action, while the second stem-loop is essential for antisilencing and less critical for RpoS translation. The third stem-loop, which behaves as a transcription terminator, can be substituted by the trp transcription terminator without loss of either DsrA function. The sequence of the first stem-loop of DsrA is complementary with the upstream leader portion of RpoS messenger RNA, suggesting that p (en) |
| rdfs:label | DsrA RNA (en) |
| owl:sameAs | freebase:DsrA RNA wikidata:DsrA RNA https://global.dbpedia.org/id/4jF3i |
| prov:wasDerivedFrom | wikipedia-en:DsrA_RNA?oldid=1079708359&ns=0 |
| foaf:depiction | wiki-commons:Special:FilePath/RF00014.jpg |
| foaf:isPrimaryTopicOf | wikipedia-en:DsrA_RNA |
| is dbo:wikiPageWikiLink of | dbr:RprA_RNA dbr:GcvB_RNA dbr:Hfq_binding_sRNA dbr:RpoS dbr:RpoS_mRNA_5′UTR |
| is foaf:primaryTopic of | wikipedia-en:DsrA_RNA |
