DyLight Fluor (original) (raw)
DyLight Fluor é uma família de corantes fluorescentes produzidos pela empresa em colaboração com . Anticorpos conjugados a corantes DyLight são produzidas pela Thermo Fisher Scientific aasim como por diversos parceiros, incluindo , Rockland Immunochemicals, Inc., AbD Serotec,e KPL.Corantes DyLight são tipicamente usados em biotecnologia e pesquisa como biomoléculas, marcação de tecidos e células para microscopia de fluorescência, biologia celular ou biologia molecular.
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| dbo:abstract | The DyLight Fluor family of fluorescent dyes are produced by in collaboration with Thermo Fisher Scientific. DyLight dyes are typically used in biotechnology and research applications as biomolecule, cell and tissue labels for fluorescence microscopy, cell biology or molecular biology. Historically, fluorophores such as fluorescein, rhodamine, Cy3 and Cy5 have been used in a wide variety of applications. These dyes have limitations for use in microscopy and other applications that require exposure to an intense light source such as a laser, because they photobleach quickly (however, bleaching can be reduced at least 10 fold using oxygen scavenging). DyLight Fluors have comparable excitation and emission spectra and are claimed to be more photostable, brighter, and less pH-sensitive. The excitation and emission spectra of the DyLight Fluor series cover much of the visible spectrum and extend into the infrared region, allowing detection using most fluorescence microscopes, as well as infrared imaging systems. To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively. DyLight Fluors are synthesized through sulfonate addition to coumarin, xanthene (such as fluorescein and rhodamine), and cyanine dyes. Sulfonation makes DyLight dyes negatively charged and hydrophilic. DyLight Fluors are commercially available as reactive succinimidyl-esters for labeling proteins through lysine residues, and as maleimide derivatives for labeling proteins through cysteine residues. Antibodies conjugated are also available from several companies. Similar lines of fluorescent dyes provide an alternative to the DyLight Dyes (see also the list in Category:Fluorescent dyes). (en) DyLight Fluor é uma família de corantes fluorescentes produzidos pela empresa em colaboração com . Anticorpos conjugados a corantes DyLight são produzidas pela Thermo Fisher Scientific aasim como por diversos parceiros, incluindo , Rockland Immunochemicals, Inc., AbD Serotec,e KPL.Corantes DyLight são tipicamente usados em biotecnologia e pesquisa como biomoléculas, marcação de tecidos e células para microscopia de fluorescência, biologia celular ou biologia molecular. (pt) DyLight Fluor是由和赛默飞世尔科技合作开发的系列荧光染料，具有光谱宽度广、荧光强度高、光学稳定性好、对pH不敏感、分子较小渗透性好等优点，结合荧光显微镜等设备，可应用于生物医学研究中对组织、细胞和生物分子等的标记、定位 。 DyLight系列染料通过化学合成方法在香豆素、氧杂蒽(如荧光素和罗丹明)、青色素等染料中引入磺酸酯基团。硫磺酯基团使得DyLight染料呈负电性和亲水性。多数商业化的DyLight染料通过琥珀酰亚胺酯与赖氨酸残基，通过顺丁烯二酰亚胺衍生物与半胱氨酸残基结合，从而标记底物蛋白。另外也有部分公司出售结合抗体的染料。 传统的荧光染料如荧光素、罗丹明、Cy3和Cy5等通过去除氧化物等工艺可使半衰期至少增加10倍，但是在暴露于荧光显微镜的激光束等强光源后仍容易发生荧光淬灭，从而限制了它们应用。DyLight系列荧光染料具有更好的光学稳定性、发光更持久。其荧光成像包括了最常用的荧光颜色，激发光和发射光光谱分布合理，覆盖大部分可见光和红外线区域，适用于绝大多数荧光显微镜，并可应用于红外成像系统。其中DyLight 405在蓝色激发光下呈现紫色荧光，DyLight 488在青色 (488 nm)激发光下呈现绿色荧光，DyLight 550和594在绿色(526 nm)激发光下呈现橙黄色荧光，DyLight 633和650在红色(633 nm)激发光下呈现红色荧光。DyLight 680、755和800则适配基于激光或滤镜的红外成像系统，分别发射700 nm, 750 nm and 800 nm区域的光谱。 (zh) |
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| rdfs:comment | DyLight Fluor é uma família de corantes fluorescentes produzidos pela empresa em colaboração com . Anticorpos conjugados a corantes DyLight são produzidas pela Thermo Fisher Scientific aasim como por diversos parceiros, incluindo , Rockland Immunochemicals, Inc., AbD Serotec,e KPL.Corantes DyLight são tipicamente usados em biotecnologia e pesquisa como biomoléculas, marcação de tecidos e células para microscopia de fluorescência, biologia celular ou biologia molecular. (pt) The DyLight Fluor family of fluorescent dyes are produced by in collaboration with Thermo Fisher Scientific. DyLight dyes are typically used in biotechnology and research applications as biomolecule, cell and tissue labels for fluorescence microscopy, cell biology or molecular biology. Similar lines of fluorescent dyes provide an alternative to the DyLight Dyes (see also the list in Category:Fluorescent dyes). (en) DyLight Fluor是由和赛默飞世尔科技合作开发的系列荧光染料，具有光谱宽度广、荧光强度高、光学稳定性好、对pH不敏感、分子较小渗透性好等优点，结合荧光显微镜等设备，可应用于生物医学研究中对组织、细胞和生物分子等的标记、定位 。 DyLight系列染料通过化学合成方法在香豆素、氧杂蒽(如荧光素和罗丹明)、青色素等染料中引入磺酸酯基团。硫磺酯基团使得DyLight染料呈负电性和亲水性。多数商业化的DyLight染料通过琥珀酰亚胺酯与赖氨酸残基，通过顺丁烯二酰亚胺衍生物与半胱氨酸残基结合，从而标记底物蛋白。另外也有部分公司出售结合抗体的染料。 (zh) |
| rdfs:label | DyLight Fluor (en) DyLight Fluor (pt) DyLight Fluor (zh) |
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