| dbo:abstract |
Background: The hatchet ribozyme is an RNA structure that catalyzes its own cleavage at a specific site. In other words, it is a self-cleaving ribozyme. Hatchet ribozymes were discovered by a bioinformatics strategy as RNAs Associated with Genes Associated with Twister and Hammerhead ribozymes, or RAGATH. Subsequent biochemical analysis supports the conclusion of a ribozyme function, and determined further characteristics of the chemical reaction catalyzed by the ribozyme. Nucleolytic ribozymes are small RNAs that adopt compact folds capable of site-specific cleavage/ligation reactions. 14 unique nucleolytic ribozymes have been identified to date, including recently discovered twister, pistol, twister-sister, and hatchet ribozymes that were identified based on application of comparative sequence and structural algorithms. The consensus sequence and secondary structure of this class includes 13 highly conserved and numerous other modestly conserved nucleotides inter-dispersed among bulges linking four base-paired substructures. A representative hatchet ribozyme requires divalent cations such as Mg2+ to promote RNA strand scission with a maximum rate constant of ~4/min. As with all other small self-cleaving ribozymes discovered to date, hatchet ribozymes employ a general mechanism for catalysis consisting of a nucleophilic attack of a ribose 2′-oxygen atom on the adjacent phosphorus center. Kinetic characteristics of the reaction demonstrate that members of this ribozyme class have an essential requirement for divalent metal cations and that they have a complex active site which employs multiple catalytic strategies to accelerate RNA cleavage by internal phosphoester transfer. (en) |
| dbo:thumbnail |
wiki-commons:Special:FilePath/RF02678.svg?width=300 |
| dbo:wikiPageExternalLink |
https://2020.igem.org/Team:Hamburg/Contribution https://www.pnas.org/content/pnas/suppl/2019/05/13/1902413116.DCSupplemental/pnas.1902413116.sapp.pdf |
| dbo:wikiPageID |
52048266 (xsd:integer) |
| dbo:wikiPageLength |
13045 (xsd:nonNegativeInteger) |
| dbo:wikiPageRevisionID |
1095275889 (xsd:integer) |
| dbo:wikiPageWikiLink |
dbc:Ribozymes dbr:Ribozyme dbr:Gene dbr:GlmS_glucosamine-6-phosphate_activated_ribozyme dbr:Hairpin_ribozyme dbr:Twister_ribozyme dbr:Twister_sister_ribozyme dbc:RNA dbr:Hammerhead_ribozyme dbr:Hepatitis_delta_virus_ribozyme dbr:Subgenomic_mRNA dbr:CRISPR_interference dbr:RAGATH_RNA_motifs dbr:Sequence_conservation dbr:Short_hairpin_RNA dbr:Michaelis–Menten_kinetics dbr:Small_interfering_RNA dbr:Scissile_bond dbr:Pistol_ribozyme dbr:Watson-Crick_base_pair dbr:Secondary_structure dbr:Varkud_satellite |
| dbp:caption |
Consensus secondary structure and sequence conservation of Hatchet ribozyme (en) |
| dbp:name |
Hatchet (en) |
| dbp:rfam |
RF02678 (en) |
| dbp:rnaType |
dbr:Ribozyme dbr:Gene |
| dbp:symbol |
Hatchet (en) |
| dbp:wikiPageUsesTemplate |
dbt:Prime dbt:GO dbt:SO dbt:Infobox_rfam |
| dcterms:subject |
dbc:Ribozymes dbc:RNA |
| rdfs:comment |
Background: The hatchet ribozyme is an RNA structure that catalyzes its own cleavage at a specific site. In other words, it is a self-cleaving ribozyme. Hatchet ribozymes were discovered by a bioinformatics strategy as RNAs Associated with Genes Associated with Twister and Hammerhead ribozymes, or RAGATH. Subsequent biochemical analysis supports the conclusion of a ribozyme function, and determined further characteristics of the chemical reaction catalyzed by the ribozyme. (en) |
| rdfs:label |
Hatchet ribozyme (en) |
| owl:sameAs |
wikidata:Hatchet ribozyme https://global.dbpedia.org/id/2cvWg |
| prov:wasDerivedFrom |
wikipedia-en:Hatchet_ribozyme?oldid=1095275889&ns=0 |
| foaf:depiction |
wiki-commons:Special:FilePath/RF02678.svg |
| foaf:isPrimaryTopicOf |
wikipedia-en:Hatchet_ribozyme |
| is dbo:wikiPageDisambiguates of |
dbr:Hatchet_(disambiguation) |
| is dbo:wikiPageWikiLink of |
dbr:Ribozyme dbr:Twister_sister_ribozyme dbr:Hatchet_(disambiguation) dbr:Hammerhead_ribozyme dbr:RAGATH_RNA_motifs |
| is foaf:primaryTopic of |
wikipedia-en:Hatchet_ribozyme |
