| dbo:abstract |
Indo-1 ist ein Fluoreszenzfarbstoff, der in Anwesenheit von Calciumionen seine optischen Eigenschaften ändert und daher als Calcium-Indikator verwendet wird. (de) Indo-1 is a popular calcium indicator similar to Fura-2. In contrast to Fura-2, Indo-1 has a dual emissions peak. The main emission peak in calcium-free solution is 475 nm while in the presence of calcium the emission is shifted to 400 nm. It is widely used in flow cytometry.The penta potassium salt is commercially available and preferred to the free acid because of its higher solubility in water. While Indo-1 is not cell permeable the penta enters the cell where it is cleaved by intracellular esterases to Indo-1.The synthesis and properties of Indo-1 were presented in 1985 by the group of Roger Y Tsien. (en) Indo-1 é um composto orgânico popular similar ao . Em contraste com o Fura-2, Indo-1 tem um duplo pico de emissão. (pt) |
| dbo:alternativeName |
Indo-1 (en) |
| dbo:iupacName |
2-[4-(bis(carboxymethyl)amino)-3-[2-[2-(bis(carboxymethyl)amino)-]5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid (en) |
| dbo:thumbnail |
wiki-commons:Special:FilePath/Indo-1.svg?width=300 |
| dbo:wikiPageExternalLink |
http://www.lymphozyten.de/docs/methods_ca_jenne_2003.pdf |
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| dbo:wikiPageWikiLink |
dbr:Calcium dbr:Carbon dbr:Roger_Y._Tsien dbr:Nitrogen dbr:Hydrogen dbc:Chelating_agents dbc:Fluorescent_dyes dbr:Fura-2 dbr:Esterases dbc:Cell_imaging dbr:Oxygen dbr:Flow_cytometry dbc:Biochemistry_methods dbr:Calcium_indicator dbr:Indo-1_AM dbr:Acetoxymethyl_ester |
| dbp:imagefile |
Indo-1.svg (en) |
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250 (xsd:integer) |
| dbp:iupacname |
2 (xsd:integer) |
| dbp:othernames |
Indo-1 (en) |
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443873843 (xsd:integer) |
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changed (en) |
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| dcterms:subject |
dbc:Chelating_agents dbc:Fluorescent_dyes dbc:Cell_imaging dbc:Biochemistry_methods |
| gold:hypernym |
dbr:Indicator |
| rdf:type |
owl:Thing dul:ChemicalObject dbo:ChemicalSubstance wikidata:Q11173 yago:WikicatBiochemistryMethods yago:Ability105616246 yago:AbsorptionIndicator114598383 yago:Abstraction100002137 yago:Cognition100023271 yago:ColoringMaterial114984973 yago:Dye114985383 yago:Fluorescein114987695 yago:Indicator114917208 yago:Know-how105616786 yago:Material114580897 yago:Matter100020827 yago:Method105660268 yago:Part113809207 yago:PhysicalEntity100001930 yago:PsychologicalFeature100023100 yago:Relation100031921 dbo:ChemicalCompound yago:Substance100019613 yago:WikicatFluorescentDyes umbel-rc:ChemicalSubstanceType |
| rdfs:comment |
Indo-1 ist ein Fluoreszenzfarbstoff, der in Anwesenheit von Calciumionen seine optischen Eigenschaften ändert und daher als Calcium-Indikator verwendet wird. (de) Indo-1 is a popular calcium indicator similar to Fura-2. In contrast to Fura-2, Indo-1 has a dual emissions peak. The main emission peak in calcium-free solution is 475 nm while in the presence of calcium the emission is shifted to 400 nm. It is widely used in flow cytometry.The penta potassium salt is commercially available and preferred to the free acid because of its higher solubility in water. While Indo-1 is not cell permeable the penta enters the cell where it is cleaved by intracellular esterases to Indo-1.The synthesis and properties of Indo-1 were presented in 1985 by the group of Roger Y Tsien. (en) Indo-1 é um composto orgânico popular similar ao . Em contraste com o Fura-2, Indo-1 tem um duplo pico de emissão. (pt) |
| rdfs:label |
Indo-1 (de) Indo-1 (en) Indo-1 (pt) |
| owl:sameAs |
freebase:Indo-1 yago-res:Indo-1 wikidata:Indo-1 http://azb.dbpedia.org/resource/۱-ایندو dbpedia-de:Indo-1 dbpedia-fa:Indo-1 dbpedia-pt:Indo-1 dbpedia-sh:Indo-1 dbpedia-sr:Indo-1 https://global.dbpedia.org/id/4mzgn |
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wikipedia-en:Indo-1?oldid=1059928310&ns=0 |
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wiki-commons:Special:FilePath/Indo-1.svg |
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wikipedia-en:Indo-1 |
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dbr:C32H31N3O12 |
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dbr:Calcein dbr:Roger_Y._Tsien dbr:Fura-2 dbr:Cresol dbr:Calcium_imaging dbr:C32H31N3O12 |
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wikipedia-en:Indo-1 |
