Methylation specific oligonucleotide microarray (original) (raw)
Methylation specific oligonucleotide microarray, also known as MSO microarray, was developed as a technique to map epigenetic methylation changes in DNA of cancer cells. The general process starts with modification of DNA with bisulfite, specifically to convert unmethylated cytosine in CpG sites to uracil, while leaving methylated cytosines untouched. The modified DNA region of interest is amplified via PCR and during the process, uracils are converted to thymine. The amplicons are labelled with a fluorescent dye and hybridized to oligonucleotide probes that are fixed to a glass slide. The probes differentially bind to cytosine and thymine residues, which ultimately allows discrimination between methylated and unmethylated CpG sites, respectively.
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| dbo:abstract | Methylation specific oligonucleotide microarray, also known as MSO microarray, was developed as a technique to map epigenetic methylation changes in DNA of cancer cells. The general process starts with modification of DNA with bisulfite, specifically to convert unmethylated cytosine in CpG sites to uracil, while leaving methylated cytosines untouched. The modified DNA region of interest is amplified via PCR and during the process, uracils are converted to thymine. The amplicons are labelled with a fluorescent dye and hybridized to oligonucleotide probes that are fixed to a glass slide. The probes differentially bind to cytosine and thymine residues, which ultimately allows discrimination between methylated and unmethylated CpG sites, respectively. A calibration curve is produced and compared with the microarray results of the amplified DNA samples. This allows a general quantification of the proportion of methylation present in the region of interest. This microarray technique was developed by Tim Hui-Ming Huang and his laboratory and was officially published in 2002. (en) |
| dbo:thumbnail | wiki-commons:Special:FilePath/MSO_microarray_diagram.png?width=300 |
| dbo:wikiPageExternalLink | http://www.epigeneticstation.com https://archive.today/20131011222828/http:/arraydirectory.com/methylation-array/ |
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| dbo:wikiPageWikiLink | dbr:Cancer dbr:Amplicon dbr:Methylation dbc:Cancer_research dbr:Cyanine dbr:DNA dbr:DNA_methylation dbr:Polymerase_chain_reaction dbr:Epigenetic_therapy dbr:Leukemia dbr:Tumor_suppressor dbr:Carcinogenesis dbr:CpG_site dbr:Hybridization_probe dbc:Microarrays dbr:Bisulfite dbr:File:MSO_microarray_diagram.png |
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| rdfs:comment | Methylation specific oligonucleotide microarray, also known as MSO microarray, was developed as a technique to map epigenetic methylation changes in DNA of cancer cells. The general process starts with modification of DNA with bisulfite, specifically to convert unmethylated cytosine in CpG sites to uracil, while leaving methylated cytosines untouched. The modified DNA region of interest is amplified via PCR and during the process, uracils are converted to thymine. The amplicons are labelled with a fluorescent dye and hybridized to oligonucleotide probes that are fixed to a glass slide. The probes differentially bind to cytosine and thymine residues, which ultimately allows discrimination between methylated and unmethylated CpG sites, respectively. (en) |
| rdfs:label | Methylation specific oligonucleotide microarray (en) |
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| prov:wasDerivedFrom | wikipedia-en:Methylation_specific_oligonucleotide_microarray?oldid=1119136207&ns=0 |
| foaf:depiction | wiki-commons:Special:FilePath/MSO_microarray_diagram.png |
| foaf:isPrimaryTopicOf | wikipedia-en:Methylation_specific_oligonucleotide_microarray |
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