Most probable number (original) (raw)
El método del Número más probable (NMP) (Most probable number - MPN - en inglés), también conocido como el método de los ceros de Poisson, es una forma de obtener datos cuantitativos en concentraciones de elementos discretos a partir de datos de incidencia positiva/negativa. Es una estrategia eficiente para estimar densidades de población que se emplea cuando una evaluación cuantitativa de elementos individuales no es factible.
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| dbo:abstract | El método del Número más probable (NMP) (Most probable number - MPN - en inglés), también conocido como el método de los ceros de Poisson, es una forma de obtener datos cuantitativos en concentraciones de elementos discretos a partir de datos de incidencia positiva/negativa. Es una estrategia eficiente para estimar densidades de población que se emplea cuando una evaluación cuantitativa de elementos individuales no es factible. (es) The most probable number method, otherwise known as the method of Poisson zeroes, is a method of getting quantitative data on concentrations of discrete items from positive/negative (incidence) data. There are many discrete entities that are easily detected but difficult to count. Any sort of amplification reaction or catalysis reaction obliterates easy quantification but allows presence to be detected very sensitively. Common examples include microorganism growth, enzyme action, or catalytic chemistry. The MPN method involves taking the original solution or sample, and subdividing it by orders of magnitude (frequently 10× or 2×), and assessing presence/absence in multiple subdivisions. The degree of dilution at which absence begins to appear indicates that the items have been diluted so much that there are many subsamples in which none appear. A suite of replicates at any given concentration allow finer resolution, to use the number of positive and negative samples to estimate the original concentration within the appropriate order of magnitude. In microbiology, the cultures are incubated and assessed by eye, bypassing tedious colony counting or expensive and tedious microscopic counts. Presumptive, confirmative and completed tests are a part of MPN. In molecular biology, a common application involves DNA templates diluted into polymerase chain reactions (PCR). Reactions only proceed when a template is present, allowing for a form of quantitative PCR, to assess the original concentration of template molecules. Another application involves diluting enzyme stocks into solution containing a chromogenic substrate, or diluting antigens into solutions for ELISA (Enzyme-Linked ImmunoSorbent Assay) or some other antibody cascade detection reaction, to measure the original concentration of the enzyme or antigen. The major weakness of MPN methods is the need for large numbers of replicates at the appropriate dilution to narrow the confidence intervals. However, it is a very important method for counts when the appropriate order of magnitude is unknown a priori and sampling is necessarily destructive. (en) Angka paling mungkin (bahasa Inggris: most probable number, disingkat MPN) adalah metode yang digunakan untuk menghitung perkiraan jumlah bakteri dalam suatu bahan atau makanan. Metode ini menggunakan media kultur cair dalam tabung reaksi dan menghitung jumlah tabung positif, yaitu jumlah tabung yang ditumbuhi oleh bakteri setelah inkubasi pada suhu dan waktu tertentu. Pengamatan tabung yang positif dapat dilihat dengan mengamati timbulnya kekeruhan atau terbentuknya gas yang dihasilkan pada tabung Durham yang diletakkan pada posisi terbalik oleh bakteri pembentuk gas. Metode MPN banyak digunakan dalam penghitungan sel, terutama penghitungan koliform sebagai uji kualitas mikrobiologi air, berdasarkan perkiraan terdekat yaitu perhitungan dalam rentang tertentu, dengan mengacu pada tabel MPN. (in) Il Most Probable Number (o MPN, tradotto in italiano numero più probabile) o tecnica di conteggio con tubi multipli è una tecnica analitica usata per contare la carica microbica di un campione. Si tratta di un metodo di conteggio indiretto della carica batterica, in cui i microrganismi vitali vengono contati sulla base di una manifestazione visibile della crescita (torbidità, viraggio indicatori, etc.). In tale metodo statistico, la densità dei microrganismi ricercati è ricavata da una elaborazione dei dati positivi e negativi, ottenuti da una serie di inoculi del campione di acqua in terreni colturali liquidi. Generalmente viene usato per la determinazione della colimetria totale e fecale. (it) |
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| rdfs:comment | El método del Número más probable (NMP) (Most probable number - MPN - en inglés), también conocido como el método de los ceros de Poisson, es una forma de obtener datos cuantitativos en concentraciones de elementos discretos a partir de datos de incidencia positiva/negativa. Es una estrategia eficiente para estimar densidades de población que se emplea cuando una evaluación cuantitativa de elementos individuales no es factible. (es) Il Most Probable Number (o MPN, tradotto in italiano numero più probabile) o tecnica di conteggio con tubi multipli è una tecnica analitica usata per contare la carica microbica di un campione. Si tratta di un metodo di conteggio indiretto della carica batterica, in cui i microrganismi vitali vengono contati sulla base di una manifestazione visibile della crescita (torbidità, viraggio indicatori, etc.). In tale metodo statistico, la densità dei microrganismi ricercati è ricavata da una elaborazione dei dati positivi e negativi, ottenuti da una serie di inoculi del campione di acqua in terreni colturali liquidi. Generalmente viene usato per la determinazione della colimetria totale e fecale. (it) The most probable number method, otherwise known as the method of Poisson zeroes, is a method of getting quantitative data on concentrations of discrete items from positive/negative (incidence) data. There are many discrete entities that are easily detected but difficult to count. Any sort of amplification reaction or catalysis reaction obliterates easy quantification but allows presence to be detected very sensitively. Common examples include microorganism growth, enzyme action, or catalytic chemistry. The MPN method involves taking the original solution or sample, and subdividing it by orders of magnitude (frequently 10× or 2×), and assessing presence/absence in multiple subdivisions. (en) Angka paling mungkin (bahasa Inggris: most probable number, disingkat MPN) adalah metode yang digunakan untuk menghitung perkiraan jumlah bakteri dalam suatu bahan atau makanan. Metode ini menggunakan media kultur cair dalam tabung reaksi dan menghitung jumlah tabung positif, yaitu jumlah tabung yang ditumbuhi oleh bakteri setelah inkubasi pada suhu dan waktu tertentu. Pengamatan tabung yang positif dapat dilihat dengan mengamati timbulnya kekeruhan atau terbentuknya gas yang dihasilkan pada tabung Durham yang diletakkan pada posisi terbalik oleh bakteri pembentuk gas. Metode MPN banyak digunakan dalam penghitungan sel, terutama penghitungan koliform sebagai uji kualitas mikrobiologi air, berdasarkan perkiraan terdekat yaitu perhitungan dalam rentang tertentu, dengan mengacu pada tabel MPN (in) |
| rdfs:label | Titerverfahren (de) Número más probable (es) Angka paling mungkin (in) Most probable number (it) Most probable number (en) |
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