| dbo:abstract |
Die multiple-displacement amplification (MDA), auch strand-displacement amplification (SDA) oder isothermal multiple -displacement amplification (IMDA) ist eine Methode zur Vermehrung von geringen Mengen an DNA als Ausgangsmaterial. Sie ist eine Variante der isothermalen DNA-Amplifikation. (de) Multiple displacement amplification (MDA) is a DNA amplification technique. This method can rapidly amplify minute amounts of DNA samples to a reasonable quantity for genomic analysis. The reaction starts by annealing random hexamer primers to the template: DNA synthesis is carried out by a high fidelity enzyme, preferentially Φ29 DNA polymerase. Compared with conventional PCR amplification techniques, MDA does not employ sequence-specific primers but amplifies all DNA, generates larger-sized products with a lower error frequency, and works at a constant temperature. MDA has been actively used in (WGA) and is a promising method for application to single cell genome sequencing and sequencing-based genetic studies. (en) |
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dbr:PCR |
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| rdfs:comment |
Die multiple-displacement amplification (MDA), auch strand-displacement amplification (SDA) oder isothermal multiple -displacement amplification (IMDA) ist eine Methode zur Vermehrung von geringen Mengen an DNA als Ausgangsmaterial. Sie ist eine Variante der isothermalen DNA-Amplifikation. (de) Multiple displacement amplification (MDA) is a DNA amplification technique. This method can rapidly amplify minute amounts of DNA samples to a reasonable quantity for genomic analysis. The reaction starts by annealing random hexamer primers to the template: DNA synthesis is carried out by a high fidelity enzyme, preferentially Φ29 DNA polymerase. Compared with conventional PCR amplification techniques, MDA does not employ sequence-specific primers but amplifies all DNA, generates larger-sized products with a lower error frequency, and works at a constant temperature. MDA has been actively used in (WGA) and is a promising method for application to single cell genome sequencing and sequencing-based genetic studies. (en) |
| rdfs:label |
Multidisplacement Amplification (de) Multiple displacement amplification (en) |
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freebase:Multiple displacement amplification wikidata:Multiple displacement amplification dbpedia-de:Multiple displacement amplification https://global.dbpedia.org/id/M912 |
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