| dbo:abstract |
A neurosphere is a culture system composed of free-floating clusters of neural stem cells. Neurospheres provide a method to investigate neural precursor cells in vitro. Putative neural stem cells are suspended in a medium lacking adherent substrates but containing necessary growth factors, such as epidermal growth factor and fibroblast growth factor. This allows the neural stem cells to form into the characteristic 3-D clusters. However, neurospheres are not identical to stem cells; rather, they only contain a small percent of neural stem cells. The predominant use of the neurosphere is in the neurosphere assay. However, in vitro and in vivo environments have shown to have different inductive effects on precursor cells. The creation of the neurosphere assay is highly sensitive; it is still unclear as to the exact differing effects that environment produces, relative to the in vivo environment. (en) Con il termine neurosfera, riferito alla spontanea assunzione di una forma sferica, si designa un ammasso di cellule staminali neurali coltivate in vitro. (it) Нейросфе́ра — сферообразное скопление нейрональных стволовых клеток либо in vitro (при помещении их в среду, не содержащую сыворотки). При внесении нейросферы в сыворотку клетки начинают дифференцироваться и выходить за пределы скопления. (ru) Neurosfärer kallas anhopningar av stamceller från hjärnan som växer i en artificiell cellkultur, in vitro. Neurosfärer tillåter experimentell manipulation av stamceller och har därför blivit ett vanligt instrument inom neurovetenskapen för att studera nybildning av nervceller i hjärnan. Neurosfärer består i huvudsak av en cellpopulation med den engelska beteckningen transient amplifying cells (C-celler). (sv) |
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http://www.microtissues.com/12-256-small-spheroids.htm https://web.archive.org/web/20100819075924/http:/www.natureprotocols.com/2006/08/25/neural_stem_cell_culture_neuro.php |
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Con il termine neurosfera, riferito alla spontanea assunzione di una forma sferica, si designa un ammasso di cellule staminali neurali coltivate in vitro. (it) Нейросфе́ра — сферообразное скопление нейрональных стволовых клеток либо in vitro (при помещении их в среду, не содержащую сыворотки). При внесении нейросферы в сыворотку клетки начинают дифференцироваться и выходить за пределы скопления. (ru) Neurosfärer kallas anhopningar av stamceller från hjärnan som växer i en artificiell cellkultur, in vitro. Neurosfärer tillåter experimentell manipulation av stamceller och har därför blivit ett vanligt instrument inom neurovetenskapen för att studera nybildning av nervceller i hjärnan. Neurosfärer består i huvudsak av en cellpopulation med den engelska beteckningen transient amplifying cells (C-celler). (sv) A neurosphere is a culture system composed of free-floating clusters of neural stem cells. Neurospheres provide a method to investigate neural precursor cells in vitro. Putative neural stem cells are suspended in a medium lacking adherent substrates but containing necessary growth factors, such as epidermal growth factor and fibroblast growth factor. This allows the neural stem cells to form into the characteristic 3-D clusters. However, neurospheres are not identical to stem cells; rather, they only contain a small percent of neural stem cells. (en) |
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Neurosfera (it) Neurosphere (en) Нейросфера (ru) Neurosfär (sv) |
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