NgAgo (original) (raw)
NgAgo is a single-stranded DNA (ssDNA)-guided Argonaute endonuclease, an acronym for Natronobacterium gregoryi Argonaute. NgAgo binds 5′ phosphorylated ssDNA of ~24 nucleotides (gDNA) to guide it to its target site and will make DNA double-strand breaks at the gDNA site. Like the CRISPR/Cas system, NgAgo was reported by Chunyu Han et al. to be suitable for genome editing, but this has not been replicated. In contrast to Cas9, the NgAgo–gDNA system does not require a protospacer adjacent motif (PAM).
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|---|---|
| dbo:abstract | NgAgo is a single-stranded DNA (ssDNA)-guided Argonaute endonuclease, an acronym for Natronobacterium gregoryi Argonaute. NgAgo binds 5′ phosphorylated ssDNA of ~24 nucleotides (gDNA) to guide it to its target site and will make DNA double-strand breaks at the gDNA site. Like the CRISPR/Cas system, NgAgo was reported by Chunyu Han et al. to be suitable for genome editing, but this has not been replicated. In contrast to Cas9, the NgAgo–gDNA system does not require a protospacer adjacent motif (PAM). (en) NgAgo é uma endonuclease de guiada por DNA de cadeia simples (ssDNA), um acrônimo para Argonauta Natronobacterium gregoryi. O NgAgo liga o ssDNA fosforilado em 5 'de ~ 24 nucleotídeos (gDNA) para guiá-lo ao seu local alvo e fará quebras de fita dupla no DNA no local do gDNA. Como o sistema CRISPR/Cas, o NgAgo foi descrito como adequado para edição de genoma, mas isso não foi replicado. Ao contrário do Cas9, o sistema NgAgo – gDNA não requer um motivo adjacente protospacer (PAM). (pt) |
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| dbo:wikiPageWikiLink | dbr:Endonuclease dbr:Nucleotide dbc:Genome_editing dbc:DNA dbr:Genome_editing dbr:Protein_&_Cell dbr:Protospacer_adjacent_motif dbc:Discovery_and_invention_controversies dbr:Preprint dbc:Bacterial_enzymes dbr:Argonaute dbc:EC_3.1 dbr:CRISPR dbr:Cas9 dbr:Nature_Biotechnology dbr:Natronobacterium_gregoryi dbr:Reproducibility dbr:Single-stranded_DNA dbr:Phosphorylated dbr:Double-strand_break |
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| dcterms:subject | dbc:Genome_editing dbc:DNA dbc:Discovery_and_invention_controversies dbc:Bacterial_enzymes dbc:EC_3.1 |
| rdfs:comment | NgAgo is a single-stranded DNA (ssDNA)-guided Argonaute endonuclease, an acronym for Natronobacterium gregoryi Argonaute. NgAgo binds 5′ phosphorylated ssDNA of ~24 nucleotides (gDNA) to guide it to its target site and will make DNA double-strand breaks at the gDNA site. Like the CRISPR/Cas system, NgAgo was reported by Chunyu Han et al. to be suitable for genome editing, but this has not been replicated. In contrast to Cas9, the NgAgo–gDNA system does not require a protospacer adjacent motif (PAM). (en) NgAgo é uma endonuclease de guiada por DNA de cadeia simples (ssDNA), um acrônimo para Argonauta Natronobacterium gregoryi. O NgAgo liga o ssDNA fosforilado em 5 'de ~ 24 nucleotídeos (gDNA) para guiá-lo ao seu local alvo e fará quebras de fita dupla no DNA no local do gDNA. Como o sistema CRISPR/Cas, o NgAgo foi descrito como adequado para edição de genoma, mas isso não foi replicado. Ao contrário do Cas9, o sistema NgAgo – gDNA não requer um motivo adjacente protospacer (PAM). (pt) |
| rdfs:label | NgAgo (en) NgAgo (pt) |
| owl:sameAs | wikidata:NgAgo dbpedia-pt:NgAgo https://global.dbpedia.org/id/2Mt31 yago-res:NgAgo |
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| foaf:isPrimaryTopicOf | wikipedia-en:NgAgo |
| is dbo:wikiPageRedirects of | dbr:Han_Chunyu dbr:Chunyu_Han |
| is dbo:wikiPageWikiLink of | dbr:LEAPER_gene_editing dbr:Genome_editing dbr:Han_Chunyu dbr:Chunyu_Han |
| is foaf:primaryTopic of | wikipedia-en:NgAgo |