| dbo:abstract |
بن-ستريب أو بنسلين-الستربتوميسين هو مزيج من مُركّب بنزيل البنسلين ومُركّب الستربتوميسين الذي يُستخدم على نطاق واسع في زراعة خلايا الثدييات لمنع حدوث التلوث بالبكتيريا. يتميّز مُركّب البنسلين بأنّه مضاد حيوي من فئة مضادات البيتا-لاكتام، وهو فعال في تثبيط البكتيريا إيجابية الغرام، بينما يتميّز مُركّب الستربتوميسين بأنّه مضاد حيوي من فئة الأمينوغليكوزيدات، وهو فعال ضد معظم البكتيريا سالبة الغرام. (ar) Pen-Strep (also known as penicillin-streptomycin) is a mixture of penicillin G and streptomycin that is widely used in mammalian cell culture media to prevent bacterial contamination. The solution contains 5,000 units of penicillin G (sodium salt) which acts as the active base, and 5,000 micrograms of streptomycin (sulfate) (base per milliliter), formulated in 0.85% saline. In general, 50-100 units of Pen-Strep per milliliter of media is used to avoid contamination in cell culture. Thus, the retail product is generally 100 times more concentrated. It is recommended for use in cell culture applications at a concentration of 10 ml per liter. It is the most common antibiotic solution for the culture of mammalian cells and it does not have any adverse effects on the cells themselves. It was first introduced in 1955 in cell culture. Penicillin is a beta-lactam antibiotic that is effective in inhibiting Gram-positive bacteria, whereas streptomycin is an aminoglycoside antibiotic which is effective against most Gram-negative bacteria. (en) |
| dbo:alternativeName |
Penicillin-streptomycin (en) |
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wiki-commons:Special:FilePath/Benzylpenicillin.svg?width=300 |
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dbr:Aminoglycoside dbc:Penicillins dbc:Antibiotics dbr:Gentamicin dbr:Streptomycin dbr:Β-lactam_antibiotic dbr:Gram-negative_bacteria dbr:Gram-positive_bacteria dbr:Penicillin_G dbr:Kanamycin_sulfate |
| dbp:imagecaption |
Chemical structures of pennicllin G and streptomycin (en) |
| dbp:imagefile |
Benzylpenicillin.svg (en) Streptomycin3.svg (en) |
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150 (xsd:integer) 200 (xsd:integer) |
| dbp:othernames |
Penicillin-streptomycin (en) |
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dbc:Penicillins dbc:Antibiotics |
| rdf:type |
owl:Thing dul:ChemicalObject dbo:ChemicalSubstance wikidata:Q11173 dbo:ChemicalCompound |
| rdfs:comment |
بن-ستريب أو بنسلين-الستربتوميسين هو مزيج من مُركّب بنزيل البنسلين ومُركّب الستربتوميسين الذي يُستخدم على نطاق واسع في زراعة خلايا الثدييات لمنع حدوث التلوث بالبكتيريا. يتميّز مُركّب البنسلين بأنّه مضاد حيوي من فئة مضادات البيتا-لاكتام، وهو فعال في تثبيط البكتيريا إيجابية الغرام، بينما يتميّز مُركّب الستربتوميسين بأنّه مضاد حيوي من فئة الأمينوغليكوزيدات، وهو فعال ضد معظم البكتيريا سالبة الغرام. (ar) Pen-Strep (also known as penicillin-streptomycin) is a mixture of penicillin G and streptomycin that is widely used in mammalian cell culture media to prevent bacterial contamination. The solution contains 5,000 units of penicillin G (sodium salt) which acts as the active base, and 5,000 micrograms of streptomycin (sulfate) (base per milliliter), formulated in 0.85% saline. In general, 50-100 units of Pen-Strep per milliliter of media is used to avoid contamination in cell culture. Thus, the retail product is generally 100 times more concentrated. It is recommended for use in cell culture applications at a concentration of 10 ml per liter. It is the most common antibiotic solution for the culture of mammalian cells and it does not have any adverse effects on the cells themselves. It was f (en) |
| rdfs:label |
بن-ستريب (ar) Pen-Strep (en) |
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wikidata:Pen-Strep dbpedia-ar:Pen-Strep https://global.dbpedia.org/id/6XsEk |
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wikipedia-en:Pen-Strep?oldid=1016198707&ns=0 |
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wiki-commons:Special:FilePath/Benzylpenicillin.svg wiki-commons:Special:FilePath/Streptomycin3.svg |
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wikipedia-en:Pen-Strep |
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dbr:Penicillin-streptomycin dbr:Penicillin/streptomycin |
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dbr:Penicillin-streptomycin dbr:Penicillin/streptomycin |
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wikipedia-en:Pen-Strep |
