Restriction map (original) (raw)
Restriktionskarten sind wichtige Hilfsmittel für die Analyse von DNA. Eine Restriktionskarte zeigt die Positionen der Schnittstellen einzelner Restriktionsenzyme auf der DNA von Genomen oder Plasmiden. Über die Länge der DNA-Fragmente, die beim Schneiden der DNA durch Restriktionsenzyme entstehen, können DNA-Abschnitte im Vergleich mit einer Restriktionskarte identifiziert werden.
| Property | Value |
|---|---|
| dbo:abstract | Restriktionskarten sind wichtige Hilfsmittel für die Analyse von DNA. Eine Restriktionskarte zeigt die Positionen der Schnittstellen einzelner Restriktionsenzyme auf der DNA von Genomen oder Plasmiden. Über die Länge der DNA-Fragmente, die beim Schneiden der DNA durch Restriktionsenzyme entstehen, können DNA-Abschnitte im Vergleich mit einer Restriktionskarte identifiziert werden. (de) A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular biology, restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA. There are other ways of mapping features on DNA for longer length DNA molecules, such as mapping by transduction. One approach in constructing a restriction map of a DNA molecule is to sequence the whole molecule and to run the sequence through a computer program that will find the recognition sites that are present for every restriction enzyme known. Before sequencing was automated, it would have been prohibitively expensive to sequence an entire DNA strand. To find the relative positions of restriction sites on a plasmid, a technique involving single and double restriction digests is used. Based on the sizes of the resultant DNA fragments the positions of the sites can be inferred. Restriction mapping is a very useful technique when used for determining the orientation of an insert in a cloning vector, by mapping the position of an off-center restriction site in the insert. (en) Um mapa de restrição é um mapa dos locais de restrição (corte) conhecidos dentro de uma sequência de ADN. O mapeamento restrição exige o uso de enzimas de restrição. Em biologia molecular, mapas de restrição são usadas como uma referência para se obterem ou pedaços curtos de ADN, e às vezes também para ADN mais longo, ADN genómico. Existem outras formas de recursos de mapeamento de ADN para as moléculas de ADN de comprimento mais longo, como o mapeamento por transdução. (pt) |
| dbo:wikiPageID | 6538017 (xsd:integer) |
| dbo:wikiPageLength | 4487 (xsd:nonNegativeInteger) |
| dbo:wikiPageRevisionID | 1085066913 (xsd:integer) |
| dbo:wikiPageWikiLink | dbr:Electrophoresis dbr:DNA dbr:Vector_NTI dbc:Molecular_biology dbr:DNA_sequencing dbr:Restriction_enzyme dbc:Genetics dbr:Agarose dbr:Transduction_(genetics) dbr:Phenol_extraction dbr:Ethanol_precipitation dbr:Molecular_biology dbr:Restriction_sites dbr:RFLP dbr:Supercoil |
| dbp:date | October 2016 (en) |
| dbp:reason | Sounds too much like a lab report. (en) |
| dbp:wikiPageUsesTemplate | dbt:Cleanup dbt:Other_uses dbt:Reflist |
| dct:subject | dbc:Molecular_biology dbc:Genetics |
| gold:hypernym | dbr:Map |
| rdf:type | dbo:Software |
| rdfs:comment | Restriktionskarten sind wichtige Hilfsmittel für die Analyse von DNA. Eine Restriktionskarte zeigt die Positionen der Schnittstellen einzelner Restriktionsenzyme auf der DNA von Genomen oder Plasmiden. Über die Länge der DNA-Fragmente, die beim Schneiden der DNA durch Restriktionsenzyme entstehen, können DNA-Abschnitte im Vergleich mit einer Restriktionskarte identifiziert werden. (de) Um mapa de restrição é um mapa dos locais de restrição (corte) conhecidos dentro de uma sequência de ADN. O mapeamento restrição exige o uso de enzimas de restrição. Em biologia molecular, mapas de restrição são usadas como uma referência para se obterem ou pedaços curtos de ADN, e às vezes também para ADN mais longo, ADN genómico. Existem outras formas de recursos de mapeamento de ADN para as moléculas de ADN de comprimento mais longo, como o mapeamento por transdução. (pt) A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular biology, restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA. There are other ways of mapping features on DNA for longer length DNA molecules, such as mapping by transduction. (en) |
| rdfs:label | Restriktionskarte (de) Restriction map (en) Mapa de restrição (pt) |
| owl:sameAs | freebase:Restriction map wikidata:Restriction map dbpedia-de:Restriction map dbpedia-pt:Restriction map https://global.dbpedia.org/id/4w8Cb |
| prov:wasDerivedFrom | wikipedia-en:Restriction_map?oldid=1085066913&ns=0 |
| foaf:isPrimaryTopicOf | wikipedia-en:Restriction_map |
| is dbo:wikiPageRedirects of | dbr:Restriction_mapping dbr:Restriction_maps |
| is dbo:wikiPageWikiLink of | dbr:Vector_NTI dbr:Index_of_genetics_articles dbr:Index_of_molecular_biology_articles dbr:Complemented_subspace dbr:Gene_map dbr:Gene_Designer dbr:Glossary_of_genetics dbr:Glossary_of_genetics_(M−Z) dbr:Restriction_enzyme dbr:Optical_mapping dbr:AVP_gene dbr:Agarose_gel_electrophoresis dbr:PlasMapper dbr:Restriction_mapping dbr:Restriction_maps |
| is foaf:primaryTopic of | wikipedia-en:Restriction_map |