dbo:abstract |
Ein SNAP-Tag ist ein Protein-Tag, das es ermöglicht, ein Protein in Zellen einer Zellkultur spezifisch mit einem Fluoreszenzfarbstoff mit hoher Quantenausbeute zu markieren. (de) SNAP-tag® is a self-labeling protein tag commercially available in various expression vectors. SNAP-tag is a 182 residues polypeptide (19.4 kDa) that can be fused to any protein of interest and further specifically and covalently tagged with a suitable ligand, such as a fluorescent dye. Since its introduction, SNAP-tag has found numerous applications in biochemistry and for the investigation of the function and localisation of proteins and enzymes in living cells. Compared to the current standard labelling methods used in fluorescence microscopy, the use of SNAP-tag presents significant advantages.SNAP-tag® is a registered trademark of New England Biolabs, Inc. (en) |
dbo:thumbnail |
wiki-commons:Special:FilePath/SNAP-tag.svg?width=300 |
dbo:wikiPageID |
37552738 (xsd:integer) |
dbo:wikiPageLength |
9269 (xsd:nonNegativeInteger) |
dbo:wikiPageRevisionID |
1089155329 (xsd:integer) |
dbo:wikiPageWikiLink |
dbr:Biochemistry dbr:Enzymes dbr:HaloTag dbr:Proximity_ligation_assay dbr:Protein_tag dbr:SpyCatcher dbr:O-6-methylguanine-DNA_methyltransferase dbr:Förster_resonance_energy_transfer dbc:Biochemistry_detection_methods dbr:Protein dbr:Yellow_fluorescent_protein dbr:Green_fluorescent_protein dbr:Fluorescence_microscopy dbr:Fluorescent_dye dbr:Multiprotein_complexes dbr:File:SNAP-tag.svg |
dbp:wikiPageUsesTemplate |
dbt:Cite_journal dbt:Refbegin dbt:Refend dbt:Reflist |
dct:subject |
dbc:Biochemistry_detection_methods |
gold:hypernym |
dbr:Tag |
rdf:type |
dbo:Person yago:WikicatBiochemistryMethods yago:WikicatBiologicalTechniquesAndTools yago:Ability105616246 yago:Abstraction100002137 yago:Cognition100023271 yago:Know-how105616786 yago:Method105660268 yago:PsychologicalFeature100023100 yago:Technique105665146 |
rdfs:comment |
Ein SNAP-Tag ist ein Protein-Tag, das es ermöglicht, ein Protein in Zellen einer Zellkultur spezifisch mit einem Fluoreszenzfarbstoff mit hoher Quantenausbeute zu markieren. (de) SNAP-tag® is a self-labeling protein tag commercially available in various expression vectors. SNAP-tag is a 182 residues polypeptide (19.4 kDa) that can be fused to any protein of interest and further specifically and covalently tagged with a suitable ligand, such as a fluorescent dye. Since its introduction, SNAP-tag has found numerous applications in biochemistry and for the investigation of the function and localisation of proteins and enzymes in living cells. Compared to the current standard labelling methods used in fluorescence microscopy, the use of SNAP-tag presents significant advantages.SNAP-tag® is a registered trademark of New England Biolabs, Inc. (en) |
rdfs:label |
Snap-Tag (de) SNAP-tag (en) |
owl:sameAs |
freebase:SNAP-tag yago-res:SNAP-tag wikidata:SNAP-tag dbpedia-de:SNAP-tag https://global.dbpedia.org/id/2AmCi |
prov:wasDerivedFrom |
wikipedia-en:SNAP-tag?oldid=1089155329&ns=0 |
foaf:depiction |
wiki-commons:Special:FilePath/SNAP-tag.svg |
foaf:isPrimaryTopicOf |
wikipedia-en:SNAP-tag |
is dbo:wikiPageRedirects of |
dbr:Snap-Tag dbr:CLIP-tag |
is dbo:wikiPageWikiLink of |
dbr:Chemically_induced_dimerization dbr:HaloTag dbr:Protein_tag dbr:HUH-tag dbr:Fluorescence-activating_and_absorption-shifting_tag dbr:Snap-Tag dbr:Gražvydas_Lukinavičius dbr:SmURFP dbr:CLIP-tag |
is foaf:primaryTopic of |
wikipedia-en:SNAP-tag |