Nuclearly-encoded CBP1 interacts with the 5′ end of mitochondrial cytochrome b pre-mRNA (original) (raw)
Summary
CBP1 is a nuclearly-encoded protein that is imported into mitochondria and confers stability on the mRNA for cytochrome b. Previous work has shown that CBP1 interacts with the cytochrome b transcript upstream of the coding sequence; a region encompassing some 1,100 nucleotides. The work presented here narrows the site of action of CBP1 to the distal third of this upstream sequence through analysis of mRNA produced from a novel recombinant gene containing segments of the gene for cytochrome b, cob, and the ATP synthase subunit 9 gene, olil. In a wild-type CBP1 strain, the cob-olil-cob gene produces stable, mature mRNA that is translated and contributes a portion of the cytochrome b necessary for optimal growth on non-fermentable medium.
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Authors and Affiliations
- Department of Biochemistry, University of Arizona, Biosciences West 345, 85721, Tucson, AZ, USA
Carol L. Dieckmann - Department of Molecular and Cellular Biology, University of Arizona, Biosciences West 350, 85721, Tucson, AZ, USA
Carol L. Dieckmann & Telsa M. Mittelmeier
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- Carol L. Dieckmann
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Dieckmann, C.L., Mittelmeier, T.M. Nuclearly-encoded CBP1 interacts with the 5′ end of mitochondrial cytochrome b pre-mRNA.Curr Genet 12, 391–397 (1987). https://doi.org/10.1007/BF00434815
- Received: 01 June 1987
- Issue Date: October 1987
- DOI: https://doi.org/10.1007/BF00434815