Characterization of the molybdate transport system ModABC of Staphylococcus carnosus (original) (raw)

Abstract

Transposon mutagenesis of Staphylococcus carnosus led to the identification of three genes, modABC, which encode an ABC transporter that is involved in molybdate transport. It was shown by [14C]palmitate labeling that ModA represents a lipoprotein that in gram-positive bacteria is the counterpart of the periplasmic binding proteins of gram-negative organisms. The sequence characteristics identify ModB as the integral-membrane, channel-forming protein and ModC as the ATP-binding energizer for the transport system. Mutants defective in modABC had only 0.4% of the wild-type nitrate reductase activity. Molybdate at a non-physiologically high concentration (100 μM) fully restored nitrate reductase activity, suggesting that at least one other system is able to transport molybdate, but with lower affinity. The expression of modA (and most likely of modBC) was independent of oxygen and nitrate. To date, there are no indications for molybdate-specific regulation of modABC expression since in a modB mutant, modA expression was unchanged in comparison to the wild-type.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Mikrobielle Genetik, Universität Tübingen, Waldhäuser Straße 70/8, D-72076 Tübingen, Germany e-mail: friedrich.goetz@uni-tuebingen.de, Tel.: +49-7071-2974636, Fax: +49-7071-295937, , , , , , DE
    Heike Neubauer, Iris Pantel, Per-Eric Lindgren & F. Götz

Authors

  1. Heike Neubauer
    You can also search for this author inPubMed Google Scholar
  2. Iris Pantel
    You can also search for this author inPubMed Google Scholar
  3. Per-Eric Lindgren
    You can also search for this author inPubMed Google Scholar
  4. F. Götz
    You can also search for this author inPubMed Google Scholar

Additional information

Received: 5 February 1999 / Accepted: 31 May 1999

Rights and permissions

About this article

Cite this article

Neubauer, H., Pantel, I., Lindgren, PE. et al. Characterization of the molybdate transport system ModABC of Staphylococcus carnosus.Arch Microbiol 172, 109–115 (1999). https://doi.org/10.1007/s002030050747

Download citation