Identification of polymorphic mutant alleles of CaMDR1, a major facilitator of Candida albicans which confers multidrug resistance, and its in vitro transcriptional activation (original) (raw)

Abstract

CaMDR1 (_Candida albicans M_ulti _D_rug _R_esistance) encodes a major facilitator whose expression in Saccharomyces cerevisiae confers resistance to several unrelated drugs. We describe here the identification and molecular characterization of seven mutant alleles of CaMDR1 (_CaMDR1-_1 to _1-_7). The complete sequencing of CaMDR1 alleles revealed several in-frame point mutations leading to a change in amino-acid residues where insertion/replacement of an aspartate residue in a serine-asparagine-aspartate-rich domain was most noteworthy. Interestingly, these alleles showed a distinct drug resistance profile. The expression of CaMDR1, or of its alleles, in C. albicans cells was enhanced by benomyl, methotrexate and several other unrelated drugs, and was more pronounced in at least one of the azole-resistant clinical isolates.

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Authors and Affiliations

  1. Membrane Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India e-mail: rp47@hotmail.com Fax: +91-11-6187338, , , , , , IN
    Vinita Gupta, Avmeet Kohli, Shankarling Krishnamurthy, Neeti Puri, Syed Ahmed Aalamgeer, Snehlata Panwar & R. Prasad

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  1. Vinita Gupta
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  2. Avmeet Kohli
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  3. Shankarling Krishnamurthy
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  4. Neeti Puri
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  5. Syed Ahmed Aalamgeer
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  6. Snehlata Panwar
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  7. R. Prasad
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Received: 22 April / 18 June 1998

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Gupta, V., Kohli, A., Krishnamurthy, S. et al. Identification of polymorphic mutant alleles of CaMDR1, a major facilitator of Candida albicans which confers multidrug resistance, and its in vitro transcriptional activation.Curr Genet 34, 192–199 (1998). https://doi.org/10.1007/s002940050385

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