Monoclonal antibodies directed against extracellular matrix proteins reduce the adherence of Candida albicans to HEp-2 cells (original) (raw)
Abstract
The presence of the extracellular matrix (ECM) proteins collagen types I and IV, laminin and fibronectin on the surface of HEp-2 cells was confirmed by flow cytometry using monoclonal antibodies. Monoclonal antibodies directed against these ECM proteins reduced the adherence of C. albicans ATCC 44990 to HEp-2 cells, the greatest reductions being evident in assays which incorporated anti-collagen type IV monoclonal antibody. The ability of sugaramines to inhibit the adherence of C. albicans to a variety of cell types has been demonstrated previously and the most significant reduction in C. albicans – HEp-2 adherence was in assays which incorporated 0.2M galactosamine. The combination of anti-collagen IV monoclonal antibody and galactosamine reduced the adherence of C. albicans to HEp-2 cells by approximately 70% (p < 0.05).
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Authors and Affiliations
- Medical Mycology Unit, Department of Biology, National University of Ireland, Maynooth, Co, Kildare, Ireland
Gary Cotter, Roisin Weedle & Kevin Kavanagh
Authors
- Gary Cotter
- Roisin Weedle
- Kevin Kavanagh
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Cotter, G., Weedle, R. & Kavanagh, K. Monoclonal antibodies directed against extracellular matrix proteins reduce the adherence of Candida albicans to HEp-2 cells.Mycopathologia 141, 137–142 (1998). https://doi.org/10.1023/A:1006940203962
- Issue date: March 1998
- DOI: https://doi.org/10.1023/A:1006940203962