Establishment in culture of pluripotential cells from mouse embryos (original) (raw)
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- Published: 09 July 1981
Nature volume 292, pages 154–156 (1981)Cite this article
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Abstract
Pluripotential cells are present in a mouse embryo until at least an early post-implantation stage, as shown by their ability to take part hi the formation of chimaeric animals1 and to form teratocarcinomas2. Until now it has not been possible to establish progressively growing cultures of these cells in vitro, and cell lines have only been obtained after teratocarcinoma formation in vivo. We report here the establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts. These cells are able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo. They have a normal karyotype.
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References
- Gardner, R. L. & Papaioannou, V. E. in The Early Development of Mammals (eds Balls, M. & Wild, A. E.) 107–132 (Cambridge University Press, 1975).
Google Scholar - Stevens, L. C. Devl Biol 21, 364–382 (1970).
Article CAS Google Scholar - Cole, R. J. & Paul, J. in Preimplantation Stages of Pregnancy (eds Wolstenholme, G. E. W. & O'Connor, M.) 82–122 (Churchill, London, 1965).
Google Scholar - Sherman, M. I. Cell 5, 343–349 (1975).
Article CAS Google Scholar - Atienza-Samols, S. B. & Sherman, M. I. Devl Biol 66, 220–231 (1978).
Article CAS Google Scholar - Solter, D. & Knowles, B. Proc. natn. Acad. Sci. U.S.A. 72, 5099–5102 (1975).
Article ADS CAS Google Scholar - Evans, M. J. J. Reprod. Pert. 62, 625–631 (1981).
Article CAS Google Scholar - Evans, M. J., Lovell-Badge, R. H., Stern, P. L. & Stinnakre, M.-G. INSERM Symp. 10, 115–129(1979).
Google Scholar - McLaren, A. J. Endocr. 50, 515–526 (1971).
Article CAS Google Scholar - Kaufman, M. H. in Progress in Anatomy Vol. 1 (eds Harrison, R. J. & Holmes, R. L.) 1–34 (Cambridge University Press, 1981).
Google Scholar - Stern, P. L. et al. Cell 14, 775–783 (1978).
Article CAS Google Scholar - Feizi, T. Blood Transfusion Immunohaemat. 23, 563–577 (1980).
CAS Google Scholar - Kapadia, A., Feizi, T. & Evans, M. J. Expl Cell Res. 131, 185–195 (1980).
Article Google Scholar
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Authors and Affiliations
- Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK
M. J. Evans - Department of Anatomy, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK
M. H. Kaufman
Authors
- M. J. Evans
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Evans, M., Kaufman, M. Establishment in culture of pluripotential cells from mouse embryos.Nature 292, 154–156 (1981). https://doi.org/10.1038/292154a0
- Received: 06 February 1981
- Accepted: 14 April 1981
- Issue Date: 09 July 1981
- DOI: https://doi.org/10.1038/292154a0
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