New member of the winged-helix protein family disrupted in mouse and rat nude mutations (original) (raw)

Nature volume 372, pages 103–107 (1994)Cite this article

Abstract

MUTATIONS at the nude locus of mice and rats disrupt normal hair growth and thymus development1,2, causing nude mice and rats to be immune-deficient. The mouse nude locus has been localized on chromosome 11 (refs 3, 4) within a region of < 1 megabase5. Here we show that one of the genes from this critical region, designated whn, encodes a new member of the winged-helix domain family of transcription factors6,7, and that it is disrupted on mouse nu and rat rnuN alleles. Mutant transcripts do not encode the character-istic DNA-binding domain, strongly suggesting that the whn gene is the nude gene. Mutations in winged-helix domain genes cause homeotic transformations in _Drosophila_8 and distort cell-fate deci-sions during vulval development in _Caenorhabditis elegans_9. The whn gene is thus the first member of this class of genes to be implicated in a specific developmental defect in vertebrates.

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References

  1. Lyon, M. F. & Searle, A. G. Genetic Variants and Strains of the Laboratory Mouse (Oxford Univ. Press, Oxford, UK, 1989).
    Google Scholar
  2. Hedrich, H. (ed.). Genetic Monitoring of Inbred Strains of Rats (Fischer, Stuttgart, Germany, 1990).
  3. Takahashi, Y. et al. J. exp. Med. 175, 873–876 (1992).
    Article CAS Google Scholar
  4. Lisitsyn, A. N. et al. Nature Genet. 6, 57–63 (1994).
    Article CAS Google Scholar
  5. Nehls, M. et al. Eur. J. Immun. 24, 1721–1723 (1994).
    Article CAS Google Scholar
  6. Weigel, D. & Jäckle, H. Cell 63, 455–456 (1990).
    Article CAS Google Scholar
  7. Lai, E., Clark, K. L., Burley, S. K. & Darnell, J. E. Jr Proc. natn. Acad. Sci. U.S.A. 90, 10421–10423 (1993).
    Article ADS CAS Google Scholar
  8. Weigel, D., Jürgens, G., Küttner, F., Seifer, E. & Jäckle, H. Cell 57, 645–658 (1989).
    Article CAS Google Scholar
  9. Miller, L. M., Gallegos, M. E., Morisseau, B. A. & Kim, S. K. Genes Dev. 7, 933–947 (1993).
    Article CAS Google Scholar
  10. Nehls, M., Pfeifer, D. & Boehm, T. Oncogene 9, 2169–2176 (1994).
    CAS PubMed Google Scholar
  11. Cordier, A. C. & Haumont, S. M. Am. J. Anat. 157, 227–263 (1980).
    Article CAS Google Scholar
  12. Köpf-Maier, P., Mbonenko, V. F. & Merker, H. J. Acta anat. 139, 178–190 (1990).
    Article Google Scholar
  13. van Vliet, E. et al. Eur. J. Immun. 15, 675–681 (1985).
    Article CAS Google Scholar
  14. Palacios, R. & Samaridis, J. Eur. J. Immun. 21, 109–113 (1991).
    Article CAS Google Scholar
  15. Li, C., Lusis, A. J., Sparkes, T., Tran, S. M. & Gaynor, R. Genomics 13, 658–664 (1992).
    Article CAS Google Scholar
  16. Mitchell, P. J. & Tijan, R. Science 245, 371–378 (1989).
    Article ADS CAS Google Scholar
  17. Cash, J. M. et al. Mammal. Genome 4, 37–42 (1993).
    Article CAS Google Scholar
  18. Kozak, M. Nucleic Acids Res. 15, 8125–8148 (1987).
    Article CAS Google Scholar
  19. Lai, E., Prezioso, V. R., Tao, W., Chen, W. S. & Darnell, J. E. Jr Genes Dev. 5, 416–427 (1991).
    Article CAS Google Scholar
  20. Boehm, T. et al. Proc. natn. Acad. Sci. U.S.A. 88, 4367–4371 (1991).
    Article ADS CAS Google Scholar
  21. Sanger, F., Nicklen, S. & Coulson, A. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
    Article ADS CAS Google Scholar

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Authors and Affiliations

  1. Molecular Medicine Group, Department of Medicine I, University of Freiburg, Hugstetter Strasse 55, D-79106, Freiburg, Germany
    Michael Nehls, Dietmar Pfeifer, Michael Schorpp, Hans Hedrich & Thomas Boehm
  2. Zentrale Tierversuchsanlage, Medizinische Hochschule Hannover, Konstanty-Gutschow-Strasse 8, D-30625, Hannover-Krefeld, Germany
    Hans Hedrich

Authors

  1. Michael Nehls
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  2. Dietmar Pfeifer
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  3. Michael Schorpp
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  4. Hans Hedrich
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  5. Thomas Boehm
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Nehls, M., Pfeifer, D., Schorpp, M. et al. New member of the winged-helix protein family disrupted in mouse and rat nude mutations.Nature 372, 103–107 (1994). https://doi.org/10.1038/372103a0

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