Manipulating the fatty acid composition of muscle and adipose tissue in beef cattle | British Journal of Nutrition | Cambridge Core (original) (raw)

Abstract

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Enhancing the n-3 polyunsaturated fatty acid (PUFA) content of beef is important in view of the generally saturated nature of fatty acids in ruminant meats and the negative effect this can have on human health. This study examined the effects of different sources of dietary n-3 PUFA on the performance of steers and the fatty acid composition of m. longissimus thoracis muscle and associated subcutaneous adipose tissue. Animals were fed ad libitum on grass silage plus one of four concentrates (60:40 forage:concentrate on a DM basis) containing differing sources of lipid: Megalac (16:0), lightly bruised whole linseed (18:3n-3), fish oil (20:5n-3 and 22:6n-3) and a mixture of linseed and fish oil (1:1, on an oil basis). Diets were formulated so that total dietary oil intake was 6 %, approximately half of which was from the experimental test oil. Linseed feeding not only increased the levels of 18:3n-3 in muscle phospholipid from 9·5 to 19 mg/100 g muscle but also enhanced the synthesis of 20:5n-3, the level of which increased from 10 to 15 mg/100 g muscle. Linseed also increased the proportion of 18:3n-3 in muscle neutral lipid and in adipose tissue lipids by a factor of 1·64 and 1·75 respectively. Fish oil feeding doubled the proportion of 20:5n-3 and 22:6n-3 in muscle phospholipids. The proportion of 18:1 trans in muscle neutral lipid was higher on the n-3 PUFA diets than the control diet, 0·04 and 0·02 respectively. Despite the implied modification to rumen metabolism, lipid source did not affect feed intake, growth rate, cold carcass weight or carcass fatness, but carcass conformation score was higher on fish oil treatments (P<0·05). However, total muscle fatty acid content was not different between treatments and ranged from 3·5–4·3 % of tissue weight. The increase in n-3 PUFA in the meat produced by feeding linseed or fish oil lowered the n-6:n-3 ratio but had little effect on the P:S ratio.

References

Ashes, JR, Siebert, BD, Gulati, SK, Cuthbertson, AZ& Scott, TW (1992) Incorporation of _n_-3 fatty acids of fish oil into tissue and serum lipids of ruminants. Lipids 27, 629–631.Google Scholar

BanniS, S,& Martin, JC (1998) Conjugated linoleic acid and metabolites. In Trans fatty acids in human nutrition, pp. 261–302 [W, Sebedio and W, Christie, editors]. Dundee: The Oily Press.Google Scholar

Barlow, SM, Young, FVK& Duthie, IF (1990) Nutritional recommendations for _n_-3 polyunsaturated fatty acids and the challenge to the food industry. Proceedings of the Nutrition Society 49, 13–21.CrossRefGoogle ScholarPubMed

Bauchart, D, Verite, R and Remond, B (1984) Long-chain fatty acid digestion in lactating cows fed fresh grass from spring to autumn. Canadian Journal of Animal Science 64 (Suppl. 1), 330–331.CrossRefGoogle Scholar

Cant, JP, Fredeen, AH, MacIntyre, T, Gunn, J& Crow, N (1997) Effect of fish oil and monensin on milk composition in dairy cows. Canadian Journal of Animal Science 77, 125–131.CrossRefGoogle Scholar

Chilliard, Y& Doreau, M (1997) Influence of supplementary fish oil and rumen-protected methionine on milk yield and composition in dairy cows. Journal of Dairy Research 64, 173–179.Google Scholar

Clinquart, A, Istasse, L, Dufrasne, I, Mayombo, A, van Eenaeme, C& Bienfait, JM (1991) Effects on animal performance and fat composition of two fat concentrates in diets for growing-fattening bulls. Animal Production 53, 315–320.Google Scholar

Cook, LJ, Scott, TW, Faichney, GJ& Lloyd Davies, H (1972) Fatty acid interrelationships in plasma, liver, muscle and adipose tissue of cattle fed safflower oil protected from ruminal hydrogenation. Lipids 7, 83–89.CrossRefGoogle ScholarPubMed

Dawson, JM, Buttery, PJ, Lammiman, LJ, Soar, JE& Essex, CP (1991) Nutritional and endocrinological manipulation of lean deposition in forage fed steers. British Journal of Nutritional 66, 171–185.CrossRefGoogle ScholarPubMed

Department of Health (1994) Report on health and social subjects No. 46. Nutritional aspects of cardiovascular disease. London: HMSO.Google Scholar

Dewhurst, RJ, Aston, K, Fisher, WJ, Evans, RT, Dhanoa, MS& McAllan, AB (1999) Comparison of energy and protein sources offered at low levels in grass-silage-based diets for dairy cows. Animal Science 68, 789–799.Google Scholar

Eaton, SB, Eaton, SB III, Konner, MJ& Shostak, M (1996) An evolutionary perspective enhances understanding of human nutritional requirements. Journal of Nutrition 126, 1732–1740.Google Scholar

Enser, M, Hallett, K, Hewett, B, Fursey, GAF& Wood, JD (1996) Fatty acid content and composition of English beef, lamb and pork at retail. Meat Science 42, 443–456.CrossRefGoogle Scholar

Enser, M, Scollan, ND, Choi, NJ, Kurt, E, Hallett, K& Wood, JD (1999) Effect of dietary lipid on the content of conjugated linoleic acid (CLA) in beef muscle. Animal Science 69, 143–146.CrossRefGoogle Scholar

Folch, J, Lees, M& Stanley, GHS (1957) A simple method for the isolation and purification of lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed

HarfootCG, CG, & Hazlewood, GP (1988) Lipid metabolism in the rumen In The Rumen Microbial Ecosystem pp. 285–322 [PN, Hobson, editor]. London, New York: Elsevier Applied Science.Google Scholar

Hay, JD& Morrison, WR (1972) Positional isomers of cis and trans monoenoic fatty acids from ox (steer) perinephric fat. Lipids 8, 94–95.CrossRefGoogle Scholar

Hwang, DH, Boudreau, M& Chanmugam, P (1988) Dietary linolenic acid and longer-chain _n_-3 fatty acids: comparison of effects on arachidonic acid metabolism in rats. Journal of Nutrition 118, 427–437.CrossRefGoogle ScholarPubMed

Kempster, AJ, Cook, GL& Grantley-Smith, N (1986) National estimates of the body composition of British cattle, sheep and pigs with special reference to trends in fatness. A review. Meat Science 17, 107–138.CrossRefGoogle ScholarPubMed

Kennelly, JJ (1996) The fatty acid composition of milk fat as influenced by feeding oilseeds. Animal Feed Science Technology 60, 137–152.CrossRefGoogle Scholar

Kimoto, WI, Ellis, R, Wasserman, AE, Oltjen, R& Wrenn, TR (1974) Fatty acid composition of muscle phospholipids from calves and growing and mature steers fed protected safflower oil. Journal of Food Science 39, 997–1001.Google Scholar

Koizumi, I, Suzuki, Y& Kaneko, JJ (1991) Studies on the fatty acid composition of intramuscular lipids of cattle, pigs and birds. Journal of Nutritional Science and Vitaminology 37, 545–554.Google Scholar

Mandell, IB, Buchanan-Smith, JG, Holub, BJ& Campbell, CP (1997) Effects of fish meal in beef cattle diets on growth performance, carcass characteristics and fatty acid composition of longissimus muscle. Journal of Animal Science 75, 910–919.CrossRefGoogle ScholarPubMed

Mansbridge, RJ& Blake, JS (1998) Nutritional factors affecting the fatty acid composition of bovine milk. British Journal of Nutrition 78 (Suppl. 1), S37–S47.CrossRefGoogle Scholar

Marmer, WN, Maxwell, RJ& Williams, JE (1984) Effects of dietary regimen and tissue site on bovine fatty acid profiles. Journal of Animal Science 59, 109–121.Google Scholar

McDonald, IW& Scott, TW (1977) Foods of ruminant origin with elevated content of polyunsaturated fatty acids. World Review of Nutrition and Dietetics 26, 144–207.CrossRefGoogle Scholar

Mills, EW, Comerford, JW, Hollender, C, Harpster, HW, House, B& Henning, WR (1992) Meat composition and palatability of Holstein and beef steers as influenced by forage type and protein source. Journal of Animal Science 70, 2446–2451.CrossRefGoogle ScholarPubMed

Mitchell, GE, Reed, AW& Rogers, SA (1991) Influence of feeding regime on the sensory qualities and fatty acid contents of beef steaks. Journal of Food Science 56, 1102–1103.CrossRefGoogle Scholar

Morgan, CA, Noble, RC, Cocchi, M& McCartney, R (1992) Manipulation of the fatty acid composition of pig meat lipids by dietary means. Journal of the Science of Food and Agriculture 58, 357–368.CrossRefGoogle Scholar

Nettleton, JA (1991) ω-3 fatty acids. Comparison of plant and seafood sources in human nutrition. Journal of the American Dietetic Association 91, 331–337.CrossRefGoogle ScholarPubMed

Ratnayake, WMN, Ackman, RG& Hulan, HW (1989) Effect of redfish meal enriched diets on the taste and _n_-3 PUFA of 42-day-old broiler chickens. Journal of Science Food and Agriculture 49, 59–74.Google Scholar

Scollan, ND, Fisher, WJ, Davies, DWR, FisherAV, Enser M & Wood JD AV, Enser M & Wood JD (1997). Manipulating the fatty acid composition of muscle in beef cattle. Proceedings of the British Society of Animal Science, 20.Google Scholar

Scollan, ND, Choi, NJ, Maeng, WJ, Enser, M& Wood, JD. Digestion of long chain fatty acids from differing feed sources and their effect on the rumen function of steers. Journal of Agricultural Science, Cambridge (In the Press).Google Scholar

Scott, TW& Ashes, JR (1993) Dietary lipids for ruminants: protection, utilization and effects on remodelling of skeletal muscle phospholipids. Australian Journal of Agricultural Research 44, 495–508.Google Scholar

Van Elswyk, ME, Sams, AR& Hargis, PS (1992) Composition, functionality and sensory evaluation of eggs from hens fed manhaden fish oil. Journal of Food Science 57, 342–344.CrossRefGoogle Scholar

Weber, PC, Sellmayer, A& Hrboticky, N (1993) Are we what we eat? Fatty acids and their diverse functions. In Lifestyle diseases and the human diet: a challenge to future food production. Proceedings of the 44th Annual Meeting of the European Association for Animal Production, pp. 19-27. Foulum, Denmark: National Institute of Animal Science.Google Scholar

Willett, WC, Stampfer, MJ, Manson, JE, Colditz, GA, Speizer, FE, Rosner, BA, Sampson, LA& Hennekins, CH (1993) Intake of trans fatty acids and risk of coronary heart disease among women. Lancet 341, 581–585.CrossRefGoogle ScholarPubMed

Wonsil, BJ, Herbein, JH& Watkins, BA (1994) Dietary and ruminally derived trans-18:1 fatty acids alter bovine milk lipids. Journal of Nutrition 124, 556–565.Google Scholar