Fingerprinting genomes using PCR with arbitrary primers * (original) (raw)

Journal Article

,

California Institute of Biological Research

11099 North Torrey Pines Road, La Jolla, CA 92037, USA

Search for other works by this author on:

California Institute of Biological Research

11099 North Torrey Pines Road, La Jolla, CA 92037, USA

Search for other works by this author on:

* Publication of this paper was delayed by the authors to allow simultaneous publication with a paper submitted later by another group. Nucleic Acids Research regrets that due to administrative errors the other paper, by Williams et al. , was published on pages 6531–6535 of issue 22. Both sets of authors agree that the two papers should be considered as published simultaneously and should be referred to together.

Author Notes

Published:

01 January 1990

Revision received:

30 August 1990

• PDF
• Split View
• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data

Navbar Search Filter Mobile Enter search term Search

Abstract

Simple and reproducible fingerprints of complex genomes can be generated using single arbitrarily chosen primers and the polymerase chain reaction (PCR). No prior sequence information is required. The method, arbitrarily primed PCR (AP-PCR), involves two cycles of low stringency amplification followed by PCR at higher stringency. We show that strains can be distinguished by comparing polymorphisms in genomic fingerprints. The generality of the method Is demonstrated by application to twenty four strains from five species of Staphylococcus , eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa (rice).

This content is only available as a PDF.

Author notes

* Publication of this paper was delayed by the authors to allow simultaneous publication with a paper submitted later by another group. Nucleic Acids Research regrets that due to administrative errors the other paper, by Williams et al. , was published on pages 6531–6535 of issue 22. Both sets of authors agree that the two papers should be considered as published simultaneously and should be referred to together.

© 1990 Oxford University Press

I agree to the terms and conditions. You must accept the terms and conditions.

Submit a comment

Name

Affiliations

Comment title

Comment

You have entered an invalid code

Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.

Citations

Views

Altmetric

Metrics

Total Views 3,401

1,868 Pageviews

1,533 PDF Downloads

Since 11/1/2016

×

Email alerts

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic