Kisspeptin Neurons in the Arcuate Nucleus of the Ewe Express Both Dynorphin A and Neurokinin B (original) (raw)

Journal Article

,

1Department of Physiology and Pharmacology (R.L.G.), West Virginia University, Morgantown, West Virginia 26506-9229;

*Address all correspondence and requests for reprints to: Dr. Robert L. Goodman, Department of Physiology and Pharmacology, P.O. Box 9229, West Virginia University, Morgantown, West Virginia.

Search for other works by this author on:

,

2Departments of Anatomy and Cell Biology (M.N.L.) and Physiology

Search for other works by this author on:

,

4Department of Physiology (J.T.S., M.R.J., A.P., J.I., I.J.C.), Monash University, Victoria 3880, Australia;

Search for other works by this author on:

,

3Pharmacology (L.M.C., C.V.R.d.O.), University of Western Ontario, London, Ontario, Canada N6A 5C1;

Search for other works by this author on:

,

3Pharmacology (L.M.C., C.V.R.d.O.), University of Western Ontario, London, Ontario, Canada N6A 5C1;

Search for other works by this author on:

,

Mohammad R. Jafarzadehshirazi

4Department of Physiology (J.T.S., M.R.J., A.P., J.I., I.J.C.), Monash University, Victoria 3880, Australia;

5Animal Physiology Department (M.R.J.), Tehran University, Tehran, Iran;

Search for other works by this author on:

,

4Department of Physiology (J.T.S., M.R.J., A.P., J.I., I.J.C.), Monash University, Victoria 3880, Australia;

Search for other works by this author on:

,

4Department of Physiology (J.T.S., M.R.J., A.P., J.I., I.J.C.), Monash University, Victoria 3880, Australia;

Search for other works by this author on:

,

6Unité Mixte de Recherche 6175 (A.C.), Institut National de la Recherche Agronomique/Centre National de la Recherche Scientifique, Université de Tours, Haras Nationaux, Institut Fédératif de Recherche 135, Nouzilly, France;

Search for other works by this author on:

,

7Institut National de la Santé et de la Recherche Médicale Unité 862 (P.C.), Institut Francois Magendie, F-33077 Cedex, Bordeaux, France

Search for other works by this author on:

... Show more

Published:

01 December 2007

• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

Robert L. Goodman, Michael N. Lehman, Jeremy T. Smith, Lique M. Coolen, Cleusa V. R. de Oliveira, Mohammad R. Jafarzadehshirazi, Alda Pereira, Javed Iqbal, Alain Caraty, Philippe Ciofi, Iain J. Clarke, Kisspeptin Neurons in the Arcuate Nucleus of the Ewe Express Both Dynorphin A and Neurokinin B, Endocrinology, Volume 148, Issue 12, 1 December 2007, Pages 5752–5760, https://doi.org/10.1210/en.2007-0961
Close

Navbar Search Filter Mobile Enter search term Search

Kisspeptin is a potent stimulator of GnRH secretion that has been implicated in the feedback actions of ovarian steroids. In ewes, the majority of hypothalamic kisspeptin neurons are found in the arcuate nucleus (ARC), with a smaller population located in the preoptic area. Most arcuate kisspeptin neurons express estrogen receptor-α, as do a set of arcuate neurons that contain both dynorphin and neurokinin B (NKB), suggesting that all three neuropeptides are colocalized in the same cells. In this study we tested this hypothesis using dual immunocytochemistry and also determined if kisspeptin neurons contain MSH or agouti-related peptide. To assess colocalization of kisspeptin and dynorphin, we used paraformaldehyde-fixed tissue from estrogen-treated ovariectomized ewes in the breeding season (n = 5). Almost all ARC, but no preoptic area, kisspeptin neurons contained dynorphin. Similarly, almost all ARC dynorphin neurons contained kisspeptin. In experiment 2 we examined colocalization of kisspeptin and NKB in picric-acid fixed tissue collected from ovary intact ewes (n = 9). Over three quarters of ARC kisspeptin neurons also expressed NKB, and a similar percentage of NKB neurons contained kisspeptin. In contrast, no kisspeptin neurons stained for MSH or agouti-related peptide. These data demonstrate that, in the ewe, a high percentage of ARC kisspeptin neurons also produce dynorphin and NKB, and we propose that a single subpopulation of ARC neurons contains all three neuropeptides. Because virtually all of these neurons express estrogen and progesterone re-ceptors, they are likely to relay the feedback effects of these steroids to GnRH neurons to regulate reproductive function.

Copyright © 2007 by The Endocrine Society

You do not currently have access to this article.

Personal account

• Sign in with email/username & password
• Get email alerts
• Save searches
• Purchase content
• Activate your purchase/trial code
• Add your ORCID iD

Get help with access

Institutional access

Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:

IP based access

Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.

Sign in through your institution

Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.

1. Click Sign in through your institution.
2. Select your institution from the list provided, which will take you to your institution's website to sign in.
3. When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
4. Following successful sign in, you will be returned to Oxford Academic.

If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.

Sign in with a library card

Enter your library card number to sign in. If you cannot sign in, please contact your librarian.

Society Members

Society member access to a journal is achieved in one of the following ways:

Sign in through society site

Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:

1. Click Sign in through society site.
2. When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
3. Following successful sign in, you will be returned to Oxford Academic.

If you do not have a society account or have forgotten your username or password, please contact your society.

Sign in using a personal account

Some societies use Oxford Academic personal accounts to provide access to their members. See below.

Personal account

A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.

Some societies use Oxford Academic personal accounts to provide access to their members.

Viewing your signed in accounts

Click the account icon in the top right to:

• View your signed in personal account and access account management features.
• View the institutional accounts that are providing access.

Signed in but can't access content

Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.

Institutional account management

For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.

Purchase

Short-term Access

To purchase short-term access, please sign in to your personal account above.

Don't already have a personal account? Register

Kisspeptin Neurons in the Arcuate Nucleus of the Ewe Express Both Dynorphin A and Neurokinin B - 24 Hours access

EUR €38.00

GBP £33.00

USD $41.00

Rental

This article is also available for rental through DeepDyve.

Citations

Views

Altmetric

Metrics

Total Views 4,124

3,050 Pageviews

1,074 PDF Downloads

Since 1/1/2017

Citations

527 Web of Science

×

Email alerts

More on this topic

Related articles in PubMed

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic