Lithium acetate (original) (raw)

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Lithium acetate
Names
Preferred IUPAC name Lithium acetate
Identifiers
CAS Number 546-89-4 (anhydrous) checkY6108-17-4 (dihydrate) checkY
3D model (JSmol) Interactive image
ChEBI CHEBI:CHEBI:63045 ☒N
ChemSpider 10562 ☒N
ECHA InfoCard 100.008.105 Edit this at Wikidata
EC Number 208-914-3
KEGG D08134 ☒N
MeSH C488804
PubChem CID 3474584
RTECS number AI545000
UNII H4278O5FLV checkY
CompTox Dashboard (EPA) DTXSID00883436 Edit this at Wikidata
InChI InChI=1S/C2H4O2.Li/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1 ☒NKey: XIXADJRWDQXREU-UHFFFAOYSA-M ☒NInChI=1/C2H4O2.Li/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1Key: XIXADJRWDQXREU-REWHXWOFAX
SMILES [Li+].CC(=O)[O-]
Properties
Chemical formula C2H3LiO2
Molar mass 65.98 g·mol−1
Appearance crystal
Density 1.26 g/cm3
Melting point 286 °C (547 °F; 559 K)
Solubility in water 45.0 g/100 mL[1]
Magnetic susceptibility (χ) −34.0·10−6 cm3/mol
Hazards
Occupational safety and health (OHS/OSH):
Main hazards toxic
NFPA 704 (fire diamond) NFPA 704 four-colored diamond 2 0 0
Lethal dose or concentration (LD, LC):
LD50 (median dose) 500 mg/kg (oral, mouse)
Safety data sheet (SDS) External MSDS
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). ☒N verify (what is checkY☒N ?) Infobox references

Chemical compound

Lithium acetate (CH3COOLi) is a salt of lithium and acetic acid. It is often abbreviated as LiOAc.

Lithium acetate is used in the laboratory as buffer for gel electrophoresis of DNA and RNA. It has a lower electrical conductivity and can be run at higher speeds than can gels made from TAE buffer (5-30V/cm as compared to 5-10V/cm). At a given voltage, the heat generation and thus the gel temperature is much lower than with TAE buffers, therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time. Downstream applications, such as isolation of DNA from a gel slice or Southern blot analysis, work as expected when using lithium acetate gels.

Lithium boric acid or sodium boric acid are usually preferable to lithium acetate or TAE when analyzing smaller fragments of DNA (less than 500 bp) due to the higher resolution of borate-based buffers in this size range as compared to acetate buffers.

Lithium acetate is also used to permeabilize the cell wall of yeast for use in DNA transformation. It is believed that the beneficial effect of LiOAc is caused by its chaotropic effect; denaturing DNA, RNA and proteins.[2]

  1. ^ Lide, David R. (1998). Handbook of Chemistry and Physics (87 ed.). Boca Raton, FL: CRC Press. p. 465. ISBN 0-8493-0594-2.
  2. ^ Norcum, MT (Aug 15, 1991). "Structural analysis of the high molecular mass aminoacyl-tRNA synthetase complex. Effects of neutral salts and detergents". The Journal of Biological Chemistry. 266 (23): 15398–405. doi:10.1016/S0021-9258(18)98629-1. PMID 1651330.