PTTG1 (original) (raw)

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Protein-coding gene in the species Homo sapiens

PTTG1
Identifiers
Aliases PTTG1, EAP1, HPTTG, PTTG, TUTR1, pituitary tumor-transforming 1, PTTG1 regulator of sister chromatid separation, securin, ECRAR
External IDs OMIM: 604147; MGI: 1353578; HomoloGene: 3110; GeneCards: PTTG1; OMA:PTTG1 - orthologs
Gene location (Human)Chromosome 5 (human)Chr.Chromosome 5 (human)[1]Chromosome 5 (human)Genomic location for PTTG1Genomic location for PTTG1Band5q33.3Start160,421,855 bp[1]End160,428,739 bp[1]
Gene location (Mouse)Chromosome 11 (mouse)Chr.Chromosome 11 (mouse)[2]Chromosome 11 (mouse)Genomic location for PTTG1Genomic location for PTTG1Band11|11 B1.1Start43,311,077 bp[2]End43,317,078 bp[2]
RNA expression patternBgeeHuman Mouse (ortholog)Top expressed insecondary oocyteventricular zoneleft testisganglionic eminenceright testisspermgingival epitheliumtrabecular bonebone marrowmucosa of esophagusTop expressed inciliary bodysecondary oocytezygoteretinal pigment epitheliumprimary oocyteirisbloodprimitive streakankleotic placodeMore reference expression dataBioGPSMore reference expression data
Gene ontologyMolecular function peptidase inhibitor activity cysteine-type endopeptidase inhibitor activity DNA-binding transcription factor activity SH3 domain binding protein binding DNA-binding transcription factor activity, RNA polymerase II-specific Cellular component cytoplasm nucleus cytosol Biological process chromosome segregation chromosome organization homologous chromosome segregation cellular response to DNA damage stimulus cell division spermatogenesis cell cycle anaphase-promoting complex-dependent catabolic process DNA repair regulation of transcription, DNA-templated negative regulation of peptidase activity negative regulation of endopeptidase activity regulation of transcription by RNA polymerase II transcription by RNA polymerase II negative regulation of mitotic sister chromatid separation ubiquitin-dependent protein catabolic process Sources:Amigo / QuickGO
OrthologsSpeciesHuman MouseEntrez923230939EnsemblENSG00000164611ENSMUSG00000020415UniProtO95997Q6IAL9Q9CQJ7RefSeq (mRNA)NM_004219NM_001282382NM_001282383NM_001131054NM_013917NM_001362799RefSeq (protein)NP_001269311NP_001269312NP_004210NP_001269311.1NP_001269312.1NP_004210.1NP_001124526NP_038945NP_001349728Location (UCSC)Chr 5: 160.42 – 160.43 MbChr 11: 43.31 – 43.32 MbPubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Securin is a protein that in humans is encoded by the PTTG1 gene.[5][6][7]

The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.[7]

PTTG1 has been shown to interact with:

During Mitosis CDK1 phosphorylate PTTG1 at Ser-165.[12] PTTG1 is down-regulated in melanoma cells in response to the cyclin-dependent kinase inhibitor PHA-848125.[13]

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000164611 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000020415 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Domínguez A, Ramos-Morales F, Romero F, Rios RM, Dreyfus F, Tortolero M, Pintor-Toro JA (Oct 1998). "hpttg, a human homologue of rat pttg, is overexpressed in hematopoietic neoplasms. Evidence for a transcriptional activation function of hPTTG". Oncogene. 17 (17): 2187–93. doi:10.1038/sj.onc.1202140. PMID 9811450. S2CID 24151303.
  6. ^ Zhang X, Horwitz GA, Prezant TR, Valentini A, Nakashima M, Bronstein MD, Melmed S (Jan 1999). "Structure, expression, and function of human pituitary tumor-transforming gene (PTTG)". Molecular Endocrinology. 13 (1): 156–66. doi:10.1210/me.13.1.156. PMID 9892021.
  7. ^ a b "Entrez Gene: PTTG1 pituitary tumor-transforming 1".
  8. ^ a b Pei L (Jan 1999). "Pituitary tumor-transforming gene protein associates with ribosomal protein S10 and a novel human homologue of DnaJ in testicular cells". The Journal of Biological Chemistry. 274 (5): 3151–8. doi:10.1074/jbc.274.5.3151. PMID 9915854.
  9. ^ Romero F, Multon MC, Ramos-Morales F, Domínguez A, Bernal JA, Pintor-Toro JA, Tortolero M (Mar 2001). "Human securin, hPTTG, is associated with Ku heterodimer, the regulatory subunit of the DNA-dependent protein kinase". Nucleic Acids Research. 29 (6): 1300–7. doi:10.1093/nar/29.6.1300. PMC 29753. PMID 11238996.
  10. ^ Bernal JA, Luna R, Espina A, Lázaro I, Ramos-Morales F, Romero F, Arias C, Silva A, Tortolero M, Pintor-Toro JA (Oct 2002). "Human securin interacts with p53 and modulates p53-mediated transcriptional activity and apoptosis". Nature Genetics. 32 (2): 306–11. doi:10.1038/ng997. PMID 12355087. S2CID 1770399.
  11. ^ Chien W, Pei L (Jun 2000). "A novel binding factor facilitates nuclear translocation and transcriptional activation function of the pituitary tumor-transforming gene product". The Journal of Biological Chemistry. 275 (25): 19422–7. doi:10.1074/jbc.M910105199. PMID 10781616.
  12. ^ Ramos-Morales F, Domínguez A, Romero F, Luna R, Multon MC, Pintor-Toro JA, Tortolero M (Jan 2000). "Cell cycle regulated expression and phosphorylation of hpttg proto-oncogene product". Oncogene. 19 (3): 403–9. doi:10.1038/sj.onc.1203320. PMID 10656688. S2CID 21958836.
  13. ^ Caporali S, Alvino E, Levati L, Esposito AI, Ciomei M, Brasca MG, Del Bufalo D, Desideri M, Bonmassar E, Pfeffer U, D'Atri S (Sep 2012). "Down-regulation of the PTTG1 proto-oncogene contributes to the melanoma suppressive effects of the cyclin-dependent kinase inhibitor PHA-848125". Biochemical Pharmacology. 84 (5): 598–611. doi:10.1016/j.bcp.2012.06.004. PMID 22704958.