Addgene: pMD2.G (original) (raw)

pMD2.G

(Plasmid #12259)

• Purpose
  VSV-G envelope expressing plasmid

Ordering

This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.

Backbone

• Vector backbone
  pMD2.G
• Vector type
  Mammalian Expression, Lentiviral ; Envelope

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin, 100 μg/mL
• Growth Temperature
  37°C
• Growth Strain(s)
  NEB Stable
• Copy number
  Low Copy

Gene/Insert

• Gene/Insert name
  VSV G

Cloning Information

• Cloning method Restriction Enzyme
• 5′ sequencing primer CMV Fwd (Common Sequencing Primers)

Resource Information

• Addgene Notes
  • Addgene diagnostic digest- NotI, HindIII
  • Addgene Diagnostic Digest- BamHI
  • Addgene Diagnostic Digest- PvuI-HF and ApaI
  • Addgene Diagnostic Digest- EcoRI
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid
  • 5107 References

Terms and Licenses

• Academic/Nonprofit Terms
  • UBMTA
• Industry Terms
  • Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid runs as a dimer (>11kb). While this may reduce DNA yield, the plasmid still functions as expected for viral packaging.

Envelope plasmid. Known to work with most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system.

Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

• For your Materials & Methods section:
  pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259 ; http://n2t.net/addgene:12259 ; RRID:Addgene_12259)