Gene delivery - Latest research and news (original) (raw)

Gene delivery articles from across Nature Portfolio

Gene delivery is a process by which foreign DNA is transferred to host cells for applications such as genetic research or gene therapy. Gene delivery methods can be mechanical (e.g. microinjection, electroporation or biolistics), chemical (e.g. lipid or nanoparticle carriers) or biological (e.g. viral or bacterial vectors).

Lysozyme-coated LDHs boost trait control

Coating RNA- or DNA-loaded layered double hydroxide nanosheets with lysozyme enhances their uptake by loosening the plant cell wall and stimulating endocytosis and membrane trafficking — with promising implications for both fundamental research and agricultural applications.

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Delivery of Prime editing in human stem cells using pseudoviral NanoScribes particles

Prime Editing is an advanced CRISPRCas9-based tool for precisely rewriting genes in living cells. Here, authors designed virus-like particles optimized to safely and efficiently introduce this technology into human cells of therapeutic interest.

Lysozyme-coated nanoparticles for active uptake and delivery of synthetic RNA and plasmid-encoded genes in plants

This work describes protein-coated clay nanoparticles that are actively taken up by roots to deliver synthetic RNA and DNA-encoded genes into plants. The nanoparticles can also deliver functional nucleic acids directly into other plant tissues.

The hybrid lipoplex induces cytoskeletal rearrangement via autophagy/RhoA signaling pathway for enhanced anticancer gene therapy

Delivering plasmid DNA to solid tumors is challenging since it demands more transport steps than other genetic materials. Here this group designs a hybrid lipoplex for co-delivering p53 plasmid along with MDM2 inhibitor sp141 to hepatocellular carcinoma and lung carcinoma for synergistic anti-tumor treatment.

AAV library screening identifies novel vector for efficient transduction of human aorta

Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy

A deep-learning method designs lipid nanoparticles that deliver mRNA to the ferret lung.

Intracisternal vs intraventricular injection of AAV1 result in comparable, widespread transduction of the dog brain

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News and Comment

A preprint by Xu et al. shows that MHC-pseudotyped retroviruses can reprogramme, activate and expand tumour-specific T cell populations in vivo.

Lysozyme-coated LDHs boost trait control

Coating RNA- or DNA-loaded layered double hydroxide nanosheets with lysozyme enhances their uptake by loosening the plant cell wall and stimulating endocytosis and membrane trafficking — with promising implications for both fundamental research and agricultural applications.

Therapeutic interfering particles against HIV: molecular parasites reducing viremia

A breath of fresh air: targeted non-viral in vivo gene correction in the mammalian lung

Non-viral targeted insertion of large payloads into T cells

The nuclease Cas9 and DNA-repair pathway homology-mediated end joining can be leveraged to efficiently and non-virally integrate large DNA payloads into genomic target sites in primary T cells.

Engineering good viruses to improve crop performance

Viruses can be engineered to deliver nucleic acids, peptides and proteins for plant trait reprogramming. Building on market approvals and sales of recombinant virus-based biopharmaceuticals for veterinary and human medicine, similar innovations may be applied to agriculture for transient or heritable biodesign of crops with improved performance and sustainable production.

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