Methylation of FrzCD Defines a Discrete Step in the Developmental Program of Myxococcus xanthus (original) (raw)

J Bacteriol. 1998 Nov; 180(21): 5765–5768.

Note

Yongzhi Geng

School of Dentistry and Molecular Biology Institute, University of California, Los Angeles, California 90095,1 Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019,2 and Department of Molecular and Cell Biology, University of California, Berkeley, California 947203

Zhaomin Yang

John Downard

David Zusman

Wenyuan Shi

*Corresponding author. Mailing address: School of Dentistry and Molecular Biology Institute, University of California, Los Angeles, CA 90095. Phone: (310) 825-8356. Fax: (310) 206-5539. E-mail: ude.alcu@nauynew.

Received 1998 Apr 3; Accepted 1998 Sep 4.

Abstract

Myxococcus xanthus is a gram-negative soil bacterium which undergoes fruiting body formation during starvation. The frz signal transduction system has been found to play an important role in this process. FrzCD, a methyl-accepting taxis protein homologue, shows modulated methylation during cellular aggregation, which is thought to be part of an adaptation response to an aggregation signal. In this study, we assayed FrzCD methylation in many known and newly isolated mutants defective in fruiting body formation to determine a possible relationship between the methylation response and fruiting morphology. The results of our analysis indicated that the developmental mutants could be divided into two groups based on their ability to show normal FrzCD methylation during development. Many mutants blocked early in development, i.e., nonaggregating or abnormally aggregating mutants, showed poor FrzCD methylation. The well-characterized asg, bsg, csg, and esg mutants were found to be of this type. The defects in FrzCD methylation of these signaling mutants could be partially rescued by extracellular complementation with wild-type cells or addition of chemicals which restore their fruiting body formation. Mutants blocked in late development, i.e., translucent mounds, showed normal FrzCD methylation. Surprisingly, some mutants blocked in early development also exhibited a normal level of FrzCD methylation. The characterized mutants in this group were found to be defective in social motility. This indicates that FrzCD methylation defines a discrete step in the development of M. xanthus and that social motility mutants are not blocked in these early developmental steps.

Myxococcus xanthus is a gram-negative bacterium which commonly grows in damp soil, on animal dung, or in other natural habitats rich in organic matter (4). The bacteria lyse, digest, and live on other microorganisms (e.g., Escherichia coli) but can also be grown on a mixture of amino acids or complex peptides. When nutrients are abundant, the bacteria swarm as a thin spreading colony on a solid surface. When deprived of nutrients, the cells aggregate to form mounds of approximately 100,000 cells. With continued starvation, the aggregated cells develop into metabolically dormant spherical myxospores.

The developmental process of M. xanthus involves directed cell movements which are controlled by the frz signal transduction system (4, 22). The frz system was discovered through characterization of a group of mutants which formed tangled frizzy filaments under fruiting conditions instead of the normal fruiting bodies (28). Sequence analysis revealed that the frz genes are homologous to chemotaxis genes (12, 14, 15). For example, FrzA was homologous to CheW, FrzE was homologous to both CheA and CheY, FrzF was homologous to CheR, and FrzG was homologous to CheB. FrzCD is homologous to the C-terminal part of methyl-accepting chemotaxis proteins of enteric bacteria, especially Tar, the receptor for aspartate in E. coli (12). The methylation of these receptor proteins in enteric bacteria is catalyzed by a specific methyltransferase, CheR, which modifies the methyl-accepting chemotaxis proteins at specific glutamate residues with _S_-adenosylmethionine as a methyl donor (7). FrzCD was found to be methylated at the homologous glutamate residues and _S_-adenosylmethionine was found to be the methyl donor. The methylation was catalyzed by the CheR homologue, FrzF (14).

A correlation between directed cell movement and chemical modification of FrzCD was established (11, 17, 18). Attractants were found to cause methylation of FrzCD, while repellents cause demethylation of FrzCD. Furthermore, it was found that over the course of development, cells aggregated; at this time, FrzCD became more methylated, indicating that a signal(s) might be produced and sensed by starved M. xanthus cells (13, 17). Even though the chemical nature of the developmental attractant(s) is still unknown, studies suggested that the putative developmental signal(s) was produced by developmental cells during fruiting body formation in a cell density-dependent manner and that the signals were sensed by the frz system to suppress cellular reversal frequencies and make cells aggregate together (20). Recently, Sogaard-Andersen and Kaiser (24) reported that the csg mutant did not exhibit FrzCD methylation during development, suggesting an interesting relationship between the frz mutants and other developmental mutants. In this study, we further investigated the role of FrzCD methylation in development by screening many known and newly isolated mutants defective in fruiting body formation. The results indicated that the methylation of FrzCD defines a discrete step in the developmental program of M. xanthus; some mutants were blocked before that step and some were blocked after.

Bacterial strains, culture conditions, and experimental procedures.

The bacterial strains used in this study are listed in Table 1. M. xanthus was grown and maintained at 32°C in CYE medium (1). Other media used in this study include MOPS (morpholinepropanesulfonic acid) medium (10 mM MOPS, pH 7.6, and 8 mM MgSO4) and CF medium (6). Either P1::Tn_5 lac_ or P4::Tn_5_kan903 (courtesy of Bryan Julien at Stanford University) was used for transposon mutagenesis as described previously (9). Myxophage Mx4 was used for generalized transduction (16). For fruiting body formation, cells (∼5 × 108/ml were placed on MOPS or CF plates (1.5% agar) and incubated at 32°C for 2 to 3 days. For the examination of developmental spores, M. xanthus cells were spotted on to CF plates and incubated at 32°C for 7 days. Spore formation was then examined by light microscopy. The spores are refractile spherical cells which are resistant to 1% sodium dodecyl sulfate. Cell motility was assayed by time-lapse video microscopy as described by Shi and Zusman (21). The assays for FrzCD methylation were performed by the methods described previously (14, 19).

TABLE 1

Bacterial strains and FrzCD methylation during development

Strain	Phenotype or genotype	FrzCD methylation during developmenta	Reference
DZ2	Wild type	+
DK1622	Wild type	+
DK5057	asg; nonaggregating	−	10
DK5209	bsg; nonaggregating	−	5
DK2630	csg; abnormally aggregating	−	23
JD300	esg; nonaggregating	−	2
SW201	Nonaggregating	−	This study
SW280	Nonaggregating	−	This study
SW282	Nonaggregating	−	This study
SW129	Abnormally aggregating	−	This study
SW174	Abnormally aggregating	−	This study
SW178	Cell density-dependent aggregation	−	This study
SW160	Translucent mounds	+	This study
SW194	Translucent mounds	+	This study
SW107	Defective in aggregation and social motility	+	26
SW164	Defective in aggregation and social motility	+	26
SW101	Defective in aggregation and social motility	+	27
SW131	Nonaggregating	+	This study
SW103	Abnormally aggregating	+	This study
SW127	Abnormally aggregating	+	This study
SW115	Cell density-dependent aggregation	+	This study

Isolation and phenotypic characterization of mutants defective in fruiting body formation.

Mutants defective in fruiting body formation were isolated following transposon mutagenesis. Strain DZF1 is wild type with regard to fruiting body formation but contains a leaky sglA locus, a gene involved in social gliding motility (1). The strain was used initially for transposon mutagenesis because it is a better host for phages. Using P4::Tn_5_kan903 and P1::Tn_5 lac_, more than 10,000 Tn_5_ insertional mutants were isolated. These mutants were streaked on CF plates and examined for cellular aggregation and fruiting body development. The linkage between the fruiting defects and the Tn_5_ insertions was established by introducing the Tn_5_ mutations back to DZF1 and to DZ2 by Mx4-mediated generalized transduction. About 200 mutants with various degrees of defects in fruiting body formation were identified. Table 1 and the legend to Fig. 1 list some of the representative mutant strains: some mutants (e.g., SW131) did not undergo any cellular morphogenesis even though they are fully motile; some mutants (e.g., SW127 and SW174) exhibited abnormal aggregation and rested at intermediate steps of fruiting body formation; some mutants (e.g., SW115) exhibited cell density-dependent behavior (no fruiting body formation at low cell density but normal at high density); some mutants (e.g., SW160) exhibited translucent mounds (forming aggregates but not spores). A number of mutants were found to be nonmotile or to exhibit frizzy filaments (data not shown). We are in the process of characterizing the genetic nature of these mutations (26, 27). In this study, these mutants, together with several other known developmental mutants, were used for analysis of FrzCD methylation.

Phenotypes of representative mutants defective in fruiting body formation. Row A shows fruiting phenotypes at a high cell density (5 × 109 cells/ml), and row B shows fruiting phenotypes at a low cell density (1 × 109 cells/ml). Pictures were taken after cells had been on MOPS medium for 72 h. Panels: A1 and B1, wild-type FB; A2 and B2, SW127; A3 and B3, SW174; A4 and B4, SW131; A5 and B5, SW115.

Two different patterns of FrzCD methylation among developmental mutants.

As reported previously (13), we found that FrzCD of wild-type cells became demethylated after 2 hours of incubation in MOPS medium (Fig. 2). After several hours of starvation, M. xanthus cells underwent developmental aggregation to form fruiting bodies. FrzCD extracted from wild-type cells after 24 h was fully methylated (Fig. 2), indicating that cells were being stimulated. We screened a number of known and newly isolated developmental mutants for FrzCD methylation in the hope of obtaining information on a possible correlation between mutant phenotypes and the FrzCD methylation step in the developmental program. As shown in Fig. 2 and Table 1, developmental mutants could be divided into two groups: the first group showed defective FrzCD methylation during development, while the second group showed normal FrzCD methylation. It is interesting that the well-characterized signaling mutants, i.e., asg, bsg, csg, and esg (2, 3, 5, 8, 10, 23), all exhibited the phenotype of the first group, defective in FrzCD methylation (Fig. 2), indicating that these mutants are blocked in the developmental program before the production or the perception of a signal(s) which normally is transduced through the frz signal transduction system. Many other nonfruiting mutants (such as SW201, SW280, SW282, SW129, SW174, and SW178) also were blocked before the FrzCD methylation step (Table 1 and Fig. 2). The strains with translucent mounds (SW160 and SW194) exhibited normal FrzCD methylation (Table 1). Interestingly, some developmental mutants (SW107, SW164, SW101, SW131, SW103, SW127, and SW115) exhibited normal FrzCD methylation even though they were not able to form fruiting bodies (Fig. 2). The characterized mutants among this group (SW107, SW164, and SW101) were observed to have defects in social motility (26, 27). We also tested several known social motility mutants and found they have normal FrzCD methylation during development (data not shown). This suggests that the social motility mutants can still produce and detect the putative signal, but because of their defects in coordinated cell movement they are unable to produce the movements needed for cellular aggregation. Some of the nonfruiting mutants listed in Table 1 (SW131, SW103, SW127, and SW115) were not defective in social motility but still showed normal FrzCD methylation. These mutants must be blocked after the signal production and detection step.

FrzCD methylation of developmental mutants. Log-phase cells were placed on MOPS buffer for 2 h (odd-numbered lanes) or 24 h (even-numbered lanes). The cells were collected for FrzCD methylation analysis with Western blotting as previously described (19). The lower bands are methylated FrzCD. Lanes: 1 and 2, wild-type FB; 3 and 4, SW131; 5 and 6, SW101; 7 and 8, SW107; 9 and 10, SW201; 11 and 12, SW164; 13 and 14, wild-type DK1622; 15 and 16, asg; 17 and 18, bsg; 19 and 20, csg; 21 and 22, esg.

Rescue of FrzCD methylation of an esg mutant by extracellular complementation with wild-type cells or addition of isovaleric acid.

Sogaard-Andersen and Kaiser (24) reported that the csg mutant which is defective in both fruiting body formation and FrzCD methylation could be rescued for both mutant phenotypes by addition of purified C factor. These results suggested that C factor plays a role in signal transduction through the frz pathway. We therefore investigated the phenotypic rescue of another signaling mutant, esg, which is also blocked in the developmental program (2, 3, 25). The esg mutant, like the csgA mutant, is defective in fruiting body formation and can be rescued when mixed with wild-type cells (3, 25) (Fig. 3). In addition, the esg mutant can be rescued by growth in the presence of isovaleric acid (3, 25) (Fig. 3). As shown in Fig. 3, under the conditions of extracellular complementation with wild-type cells or growth in the presence of isovaleric acid, FrzCD of the esg mutant also became methylated. We also found that the defect of FrzCD methylation of asg and bsg mutants can be partially rescued by extracellular complementation with wild-type cells (data not shown). These results show that many mutants blocked in fruiting body formation are defective in FrzCD methylation during development. Since C signaling depends on prior A signaling, B signaling, and E signaling, it is possible that the defects of FrzCD methylation of asg, bsg, and esg mutants are due to blocked C signaling or a signaling step later than C signaling. In any case, since the methylation of FrzCD proceeds once the block is overcome, it can serve as a very useful developmental marker to evaluate the phenotype of fruiting mutants.

Correlation between fruiting body formation and FrzCD methylation in an esg mutant JD300. Shown are the fruiting phenotypes of JD300 (A), wild-type DK1622 (B), a 1:1 mixture of DK1622 and JD300 (C), and JD300 grown in the presence of 1 mM isovaleric acid (D). (E) FrzCD methylation after 24 h of starvation. Lanes 1 to 4 contain JD300, wild-type DK1622, a 1:1 mixture of DK1622 and JD300, and JD300 grown in the presence of 1 mM isovaleric acid, respectively. The lower bands are methylated FrzCD.

It should be noted that we recently identified a new genetic locus which also encodes genes homologous to chemotaxis genes (27). Mutants defective in these new chemotaxis protein homologues were found to have a normal frz signaling pathway (including FrzCD methylation) but to be defective in social motility. We are currently interested in investigating further the FrzCD methylation-dependent and -independent processes and the interactions between them.

Acknowledgments

We thank Y. W. Han and Y. Li for very helpful discussions and suggestions. We also thank Bryan Julien for kindly providing Tn_5_kan903.

This work was supported by NIH grant GM54666 to W. Shi, NIH grant GM20509 to D. R. Zusman, NIH training grant 5-T32-AI-07323 to Z. Yang, and a grant from the Oklahoma Center for the Advancement of Science and Technology to J. Downard.

REFERENCES

1. Campos J M, Geisselsoder J, Zusman D R. Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus. J Mol Biol. 1978;119:167–178. [PubMed] [Google Scholar]

2. Downard J, Ramaswamy S V, Kil K-S. Identification of esg, a genetic locus involved in cell-cell signaling during Myxococcus xanthus development. J Bacteriol. 1993;175:7762–7770. [PMC free article] [PubMed] [Google Scholar]

3. Downard J, Toal D R. Branched-chain fatty acids: the case for a novel form of cell-cell signalling during Myxococcus xanthus development. Mol Microbiol. 1995;16:171–175. [PubMed] [Google Scholar]

4. Dworkin M, Kaiser D, editors. Myxobacteria II. Washington, D.C: American Society for Microbiology; 1993. [Google Scholar]

5. Gill R E, Cull M G. Control of developmental gene expression by cell-to-cell interaction in Myxococcus xanthus. J Bacteriol. 1986;168:341–347. [PMC free article] [PubMed] [Google Scholar]

6. Hagen D C, Bretscher A P, Kaiser D. Synergism between morphogenetic mutants of Myxococcus xanthus. Dev Biol. 1978;64:284–296. [PubMed] [Google Scholar]

7. Hazelbauer G L, Yaghmai R, Burrows G G, Baumgartner J W, Dutton D P, Morgan D G. Transducers: transmembrane receptor proteins involved in bacterial chemotaxis. In: Armitage J P, Lackie J M, editors. Biology of the chemotactic response. Cambridge, United Kingdom: Cambridge University Press; 1990. pp. 107–134. [Google Scholar]

8. Kaiser D, Losick R. How and why bacteria talk to each other. Cell. 1993;73:873–885. [PubMed] [Google Scholar]

9. Kuner J M, Kaiser D. Introduction of transposon Tn5 into Myxococcus for analysis of developmental and other nonselectable mutants. Proc Natl Acad Sci USA. 1981;78:425–429. [PMC free article] [PubMed] [Google Scholar]

10. Kuspa A, Kaiser D. Genes required for developmental signaling in Myxococcus xanthus: three asg loci. J Bacteriol. 1989;171:2762–2772. [PMC free article] [PubMed] [Google Scholar]

11. McBride M J, Köhler T, Zusman D R. Methylation of FrzCD, a methyl-accepting taxis protein of Myxococcus xanthus, is correlated with factors affecting cell behavior. J Bacteriol. 1992;174:4246–4257. [PMC free article] [PubMed] [Google Scholar]

12. McBride M J, Weinberg R A, Zusman D R. “Frizzy” aggregation genes of the gliding bacterium Myxococcus xanthus show sequence similarities to the chemotaxis genes of enteric bacteria. Proc Natl Acad Sci USA. 1989;86:424–428. [PMC free article] [PubMed] [Google Scholar]

13. McBride M J, Zusman D R. FrzCD, a methyl-accepting taxis protein from Myxococcus xanthus, shows modulated methylation during fruiting body formation. J Bacteriol. 1993;175:4936–4940. [PMC free article] [PubMed] [Google Scholar]

14. McCleary W R, McBride M J, Zusman D R. Developmental sensory transduction in Myxococcus xanthus involves methylation and demethylation of FrzCD. J Bacteriol. 1990;172:4877–4887. [PMC free article] [PubMed] [Google Scholar]

15. McCleary W R, Zusman D R. FrzE of Myxococcus xanthus is homologous to both CheA and CheY of Salmonella typhimurium. Proc Natl Acad Sci USA. 1990;87:5898–5902. [PMC free article] [PubMed] [Google Scholar]

16. O’Connor K A, Zusman D R. Genetic analysis of Myxococcus xanthus and isolation of gene replacements after transduction under conditions of limited homology. J Bacteriol. 1986;167:744–748. [PMC free article] [PubMed] [Google Scholar]

17. Shi W, Köhler T, Zusman D R. Chemotaxis plays a role in the social behavior of Myxococcus xanthus. Mol Microbiol. 1993;9:601–611. [PubMed] [Google Scholar]

18. Shi W, Köhler T, Zusman D R. Isolation and phenotypic characterization of Myxococcus xanthus mutants which are defective in sensing negative stimuli. J Bacteriol. 1994;176:696–701. [PMC free article] [PubMed] [Google Scholar]

19. Shi W, Köhler T, Zusman D. Motility and chemotaxis in Myxococcus xanthus. In: Adolph K W, editor. Molecular microbiology techniques. Vol. 3. San Diego, Calif: Academic Press; 1994. pp. 258–269. [Google Scholar]

20. Shi W, Ngok F K, Zusman D R. Cell density regulates reversal frequency in Myxococcus xanthus. Proc Natl Acad Sci USA. 1996;93:4142–4146. [PMC free article] [PubMed] [Google Scholar]

21. Shi W, Zusman D R. The two motility systems of Myxococcus xanthus show different selective advantages on various surfaces. Proc Natl Acad Sci USA. 1993;90:3378–3382. [PMC free article] [PubMed] [Google Scholar]

22. Shi W, Zusman D R. The frz signal transduction system controls multicellular behavior in Myxococcus xanthus. In: Hoch J A, Silhavy T J, editors. Two-component signal transduction. Washington, D.C: American Society for Microbiology; 1995. pp. 419–430. [Google Scholar]

23. Shimkets L J, Gill R E, Kaiser D. Developmental cell interactions in Myxococcus xanthus and the spoC locus. Proc Natl Acad Sci USA. 1983;80:1406–1410. [PMC free article] [PubMed] [Google Scholar]

24. Sogaard-Andersen L, Kaiser D. C factor, a cell-surface-associated intercellular signaling protein, stimulates the cytoplasmic Frz signal transduction system in Myxococcus xanthus. Proc Natl Acad Sci USA. 1996;93:2675–2679. [PMC free article] [PubMed] [Google Scholar]

25. Toal D R, Clifton S W, Roe B A, Downard J. The esg locus of Myxococcus xanthus encodes the E1α and E1β subunits of a branched-chain keto acid dehydrogenase. Mol Microbiol. 1995;16:177–189. [PubMed] [Google Scholar]

26. Yang Z, Geng Y, Shi W. A DnaK homolog in Myxococcus xanthus is involved in social motility and fruiting body formation. J Bacteriol. 1998;180:218–224. [PMC free article] [PubMed] [Google Scholar]

27. Yang, Z., Y. Geng, D. Xu, H. Kaplan, and W. Shi. A new set of chemotaxis homologs is essential for Myxococcus xanthus social motility. Mol. Microbiol., in press. [PubMed]

28. Zusman D R. Frizzy mutants, a new class of aggregation-defective developmental mutants of Myxococcus xanthus. J Bacteriol. 1982;150:1430–1437. [PMC free article] [PubMed] [Google Scholar]

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