Rapid breakdown of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in rat hepatocytes stimulated by vasopressin and other Ca2+-mobilizing hormones (original) (raw)

Abstract

Rat hepatocytes rapidly incorporate [32P]Pi into phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2]; their monoester phosphate groups approach isotopic equilibrium with the cellular precursor pools within 1 h. Upon stimulation of these prelabelled cells with Ca2+-mobilizing stimuli (V1-vasopressin, angiotensin, alpha 1-adrenergic, ATP) there is a rapid fall in the labelling of PtdIns4P and PtdIns(4,5)P2. Pharmacological studies suggest that each of the four stimuli acts at a different population of receptors. Insulin, glucagon and prolactin do not provoke disappearance of labelled PtdIns4P and PtdIns(4,5)P2. The labelling of PtdIns4P and PtdIns(4,5)P2 in cells stimulated with vasopressin or angiotensin initially declines at a rate of 0.5-1.0% per s, reaches a minimum after 1-2 min and then returns towards the initial value. The dose-response curves for the vasopressin- and angiotensin-stimulated responses lie close to the respective receptor occupation curves, rather than at the lower hormone concentrations needed to evoke activation of glycogen phosphorylase. Disappearance of labelled PtdIns4P and PtdIns(4,5)P2 is not observed when cells are incubated with the ionophore A23187. The hormone-stimulated polyphosphoinositide disappearance is reduced, but not abolished, in Ca2+-depleted cells. These hormonal effects are not modified by 8-bromo cyclic GMP, cycloheximide or delta-hexachlorocyclohexane. The absolute rate of polyphosphoinositide breakdown in stimulated cells is similar to the rate previously reported for the disappearance of phosphatidylinositol [Kirk, Michell & Hems (1981) Biochem. J. 194, 155-165]. It seems likely that these changes in polyphosphoinositide labelling are caused by hormonal activation of the breakdown of PtdIns(4,5)P2 (and may be also PtdIns4P) by the action of a polyphosphoinositide phosphodiesterase. We therefore suggest that the initial response to hormones is breakdown of PtdIns(4,5)P2 (and PtdIns4P?), and that the simultaneous disappearance of phosphatidylinositol might be a result of its consumption for the continuing synthesis of polyphosphoinositides.

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Selected References

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  1. Abdel-Latif A. A., Akhtar R. A., Hawthorne J. N. Acetylcholine increases the breakdown of triphosphoinositide of rabbit iris muscle prelabelled with [32P] phosphate. Biochem J. 1977 Jan 15;162(1):61–73. doi: 10.1042/bj1620061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Akhtar R. A., Abdel-Latif A. A. Requirement for calcium ions in acetylcholine-stimulated phosphodiesteratic cleavage of phosphatidyl-myo-inositol 4,5-bisphosphate in rabbit iris smooth muscle. Biochem J. 1980 Dec 15;192(3):783–791. doi: 10.1042/bj1920783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Allan D., Thomas P. The effects of Ca2+ and Sr2+ on Ca2+-sensitive biochemical changes in human erythrocytes and their membranes. Biochem J. 1981 Sep 15;198(3):441–445. doi: 10.1042/bj1980441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Aub D. L., McKinney J. S., Putney J. W., Jr Nature of the receptor-regulated calcium pool in the rat parotid gland. J Physiol. 1982 Oct;331:557–565. doi: 10.1113/jphysiol.1982.sp014391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Berridge M. J. 5-Hydroxytryptamine stimulation of phosphatidylinositol hydrolysis and calcium signalling in the blowfly salivary gland. Cell Calcium. 1982 Oct;3(4-5):385–397. doi: 10.1016/0143-4160(82)90025-2. [DOI] [PubMed] [Google Scholar]
  6. Berridge M. J., Downes C. P., Hanley M. R. Lithium amplifies agonist-dependent phosphatidylinositol responses in brain and salivary glands. Biochem J. 1982 Sep 15;206(3):587–595. doi: 10.1042/bj2060587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Berridge M. J. Phosphatidylinositol hydrolysis: a multifunctional transducing mechanism. Mol Cell Endocrinol. 1981 Nov;24(2):115–140. doi: 10.1016/0303-7207(81)90055-1. [DOI] [PubMed] [Google Scholar]
  8. Berridge M. J. The interaction of cyclic nucleotides and calcium in the control of cellular activity. Adv Cyclic Nucleotide Res. 1975;6:1–98. [PubMed] [Google Scholar]
  9. Berthon B., Poggioli J., Capiod T., Claret M. Effect of the alpha-agonist noradrenaline on total and 45Ca2+ movements in mitochondria of rat liver cells. Biochem J. 1981 Oct 15;200(1):177–180. doi: 10.1042/bj2000177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Billah M. M., Lapetina E. G. Degradation of phosphatidylinositol-4,5-bisphosphate is insensitive to CA2+ mobilization in stimulated platelets. Biochem Biophys Res Commun. 1982 Nov 16;109(1):217–222. doi: 10.1016/0006-291x(82)91587-x. [DOI] [PubMed] [Google Scholar]
  11. Billah M. M., Lapetina E. G. Rapid decrease of phosphatidylinositol 4,5-bisphosphate in thrombin-stimulated platelets. J Biol Chem. 1982 Nov 10;257(21):12705–12708. [PubMed] [Google Scholar]
  12. Billah M. M., Michell R. H. Phosphatidylinositol metabolism in rat hepatocytes stimulated by glycogenolytic hormones. Effects of angiotensin, vasopressin, adrenaline, ionophore A23187 and calcium-ion deprivation. Biochem J. 1979 Sep 15;182(3):661–668. doi: 10.1042/bj1820661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Bréant B., Keppens S., De Wulf H. Heterologous desensitization of the cyclic AMP-independent glycogenolytic response in rat liver cells. Biochem J. 1981 Dec 15;200(3):509–514. doi: 10.1042/bj2000509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Burgess G. M., Claret M., Jenkinson D. H. Effects of quinine and apamin on the calcium-dependent potassium permeability of mammalian hepatocytes and red cells. J Physiol. 1981 Aug;317:67–90. doi: 10.1113/jphysiol.1981.sp013814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Cantau B., Keppens S., De Wulf H., Jard S. (3H)-vasopressin binding to isolated rat hepatocytes and liver membranes: regulation by GTP and relation to glycogen phosphorylase activation. J Recept Res. 1980;1(2):137–168. doi: 10.3109/10799898009044096. [DOI] [PubMed] [Google Scholar]
  16. Casteels R., Droogmans G. Exchange characteristics of the noradrenaline-sensitive calcium store in vascular smooth muscle cells or rabbit ear artery. J Physiol. 1981 Aug;317:263–279. doi: 10.1113/jphysiol.1981.sp013824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Chang K. J., Triggle D. J. Quantitative aspects of drug-receptor interactions. I. Ca2+ and cholinergic receptor activation in smooth muscle: a basic model for drug-receptor interactions. J Theor Biol. 1973 Jul;40(1):125–154. doi: 10.1016/0022-5193(73)90168-9. [DOI] [PubMed] [Google Scholar]
  18. Cockcroft S. Phosphatidylinositol metabolism in mast cells and neutrophils. Cell Calcium. 1982 Oct;3(4-5):337–349. doi: 10.1016/0143-4160(82)90021-5. [DOI] [PubMed] [Google Scholar]
  19. Dawson R. M., Clarke N. D-myoinositol 1:2-cyclic phosphate 2-phosphohydrolase. Biochem J. 1972 Mar;127(1):113–118. doi: 10.1042/bj1270113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. De Torrontegui G., Berthet J. The action of insulin on the incorporation of [32P]phosphate in the phospholipids of rat adipose tissue. Biochim Biophys Acta. 1966 Jun 1;116(3):477–481. doi: 10.1016/0005-2760(66)90117-2. [DOI] [PubMed] [Google Scholar]
  21. Downes C. P., Michell R. H. The control by Ca2+ of the polyphosphoinositide phosphodiesterase and the Ca2+-pump ATPase in human erythrocytes. Biochem J. 1982 Jan 15;202(1):53–58. doi: 10.1042/bj2020053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Downes C. P., Michell R. H. The polyphosphoinositide phosphodiesterase of erythrocyte membranes. Biochem J. 1981 Jul 15;198(1):133–140. doi: 10.1042/bj1980133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Downes C. P., Mussat M. C., Michell R. H. The inositol trisphosphate phosphomonoesterase of the human erythrocyte membrane. Biochem J. 1982 Apr 1;203(1):169–177. doi: 10.1042/bj2030169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Downes P., Michell R. H. Phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate: lipids in search of a function. Cell Calcium. 1982 Oct;3(4-5):467–502. doi: 10.1016/0143-4160(82)90031-8. [DOI] [PubMed] [Google Scholar]
  25. Durell J., Garland J. T. Acetylcholine-stimulated phosphodiesteratic cleavage of phosphoinositides: hypothetical role in membrane depolarization. Ann N Y Acad Sci. 1969 Oct 17;165(2):743–754. [PubMed] [Google Scholar]
  26. Durell J., Garland J. T., Friedel R. O. Acetylcholine action: biochemical aspects. Science. 1969 Aug 29;165(3896):862–866. doi: 10.1126/science.165.3896.862. [DOI] [PubMed] [Google Scholar]
  27. Ellis R. B., Galliard T., Hawthorne J. N. Phosphoinositides. 5. The inositol lipids of ox brain. Biochem J. 1963 Jul;88(1):125–131. doi: 10.1042/bj0880125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Exton J. H. Molecular mechanisms involved in alpha-adrenergic responses. Mol Cell Endocrinol. 1981 Sep;23(3):233–264. doi: 10.1016/0303-7207(81)90123-4. [DOI] [PubMed] [Google Scholar]
  29. Fain J. N., Berridge M. J. Relationship between phosphatidylinositol synthesis and recovery of 5-hydroxytryptamine-responsive Ca2+ flux in blowfly salivary glands. Biochem J. 1979 Jun 15;180(3):655–661. doi: 10.1042/bj1800655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Farese R. V., Sabir M. A., Larson R. E. Adrenocorticotropin and adenosine 3',5'-monophosphate stimulate de novo synthesis of adrenal phosphatidic acid by a cycloheximide-sensitive, CA++-dependent mechanism. Endocrinology. 1981 Dec;109(6):1895–1901. doi: 10.1210/endo-109-6-1895. [DOI] [PubMed] [Google Scholar]
  31. Farese R. V., Sabir M. A., Larson R. E. Kinetic aspects of cycloheximide-induced reversal of adrenocorticotropin effects on steroidogenesis and adrenal phospholipids in vivo. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7189–7193. doi: 10.1073/pnas.77.12.7189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Fisher B. D., Mueller G. C. Gamma-hexachlorocyclohexane inhibits the initiation of lymphocyte growth by phytohemagglutinin. Biochem Pharmacol. 1971 Sep;20(9):2515–2518. doi: 10.1016/0006-2952(71)90255-3. [DOI] [PubMed] [Google Scholar]
  33. Frantzis N., Vakirtzi-Lemonias C. Concanavalin A receptors of the surface membrane of Crithidia fasciculata. Biochem Soc Trans. 1981 Feb;9(1):135–136. doi: 10.1042/bst0090135. [DOI] [PubMed] [Google Scholar]
  34. Garrison J. C., Borland M. K., Florio V. A., Twible D. A. The role of calcium ion as a mediator of the effects of angiotensin II, catecholamines, and vasopressin on the phosphorylation and activity of enzymes in isolated hepatocytes. J Biol Chem. 1979 Aug 10;254(15):7147–7156. [PubMed] [Google Scholar]
  35. Goldberg N. D., Haddox M. K. Cyclic GMP metabolism and involvement in biological regulation. Annu Rev Biochem. 1977;46:823–896. doi: 10.1146/annurev.bi.46.070177.004135. [DOI] [PubMed] [Google Scholar]
  36. Griffin H. D., Hawthorne J. N. Calcium-activated hydrolysis of phosphatidyl-myo-inositol 4-phosphate and phosphatidyl-myo-inositol 4,5-bisphosphate in guinea-pig synaptosomes. Biochem J. 1978 Nov 15;176(2):541–552. doi: 10.1042/bj1760541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. HOELZL J., WAGNER H. UBER DEN EINBAU VON 32P-PHOSPHAT IN DIE DI- UND TRIPHOSPHOINOSITIDE VERSCHIEDENER RATTENORGANE. Biochem Z. 1964 Jan 28;339:327–330. [PubMed] [Google Scholar]
  38. Hanson B. A., Lester R. L. The extraction of inositol-containing phospholipids and phosphatidylcholine from Saccharomyces cerevisiae and Neurospora crassa. J Lipid Res. 1980 Mar;21(3):309–315. [PubMed] [Google Scholar]
  39. Hawthorne J. N. Is phosphatidylinositol now out of the calcium gate? Nature. 1982 Jan 28;295(5847):281–282. doi: 10.1038/295281a0. [DOI] [PubMed] [Google Scholar]
  40. Hawthorne J. N., Pickard M. R. Phospholipids in synaptic function. J Neurochem. 1979 Jan;32(1):5–14. doi: 10.1111/j.1471-4159.1979.tb04503.x. [DOI] [PubMed] [Google Scholar]
  41. Hawthorne J. N., White D. A. Myo-inositol lipids. Vitam Horm. 1975;33:529–573. doi: 10.1016/s0083-6729(08)60972-3. [DOI] [PubMed] [Google Scholar]
  42. Hoffmann R., Erzberger P., Frank W., Ristow H. J. Increased phosphatidylinositol synthesis in rat embryo fibroblasts after growth stimulation and its inhibition by delta-hexachlorocyclohexane. Biochim Biophys Acta. 1980 May 28;618(2):282–292. doi: 10.1016/0005-2760(80)90034-x. [DOI] [PubMed] [Google Scholar]
  43. Hokin M. R., Brown D. F. Inhibition by gamma-hexachlorocyclohexane of acetylcholine-stimulated phosphatidylinositol synthesis in cerebral cortex slices and of phosphatidic acid-inositol transferase in cerebral cortex particulate fractions. J Neurochem. 1969 Apr;16(4):475–483. doi: 10.1111/j.1471-4159.1969.tb06846.x. [DOI] [PubMed] [Google Scholar]
  44. Jafferji S. S., Michell R. H. Effects of calcium-antagonistic drugs on the stimulation by carbamoylcholine and histamine of phosphatidylinositol turnover in longitudinal smooth muscle of guinea-pig ileum. Biochem J. 1976 Nov 15;160(2):163–169. doi: 10.1042/bj1600163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Jard S., Cantau B., Jakobs K. H. Angiotensin II and alpha-adrenergic agonists inhibit rat liver adenylate cyclase. J Biol Chem. 1981 Mar 25;256(6):2603–2606. [PubMed] [Google Scholar]
  46. Jones L. M., Cockcroft S., Michell R. H. Stimulation of phosphatidylinositol turnover in various tissues by cholinergic and adrenergic agonists, by histamine and by caerulein. Biochem J. 1979 Sep 15;182(3):669–676. doi: 10.1042/bj1820669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. KEMP P., HUBSCHER G., HAWTHORNE J. N. Phosphoinositides. 3. Enzymic hydrolysis of inositol-containing phospholipids. Biochem J. 1961 Apr;79:193–200. doi: 10.1042/bj0790193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. KFOURY G. A., KERR S. E. ON THE OCCURRENCE OF DIPHOSPHOINOSITOL IN THE LIPIDS OF LIVER AND PANCREAS. Biochim Biophys Acta. 1964 Aug 5;84:391–403. doi: 10.1016/0926-6542(64)90003-4. [DOI] [PubMed] [Google Scholar]
  49. Keppens S., De Wulf H., Clauser P., Jard S., Morgat J. L. The liver angiotensin receptor involved in the activation of glycogen phosphorylase. Biochem J. 1982 Dec 15;208(3):809–817. doi: 10.1042/bj2080809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  50. Keppens S., Vandenheede J. R., De Wulf H. On the role of calcium as second messenger in liver for the hormonally induced activation of glycogen phosphorylase. Biochim Biophys Acta. 1977 Feb 28;496(2):448–457. doi: 10.1016/0304-4165(77)90327-0. [DOI] [PubMed] [Google Scholar]
  51. Kirk C. J., Hems D. A. Hepatic action of vasopressin: lack of a role for adenosine-3',5'-cyclic monophosphate. FEBS Lett. 1974 Oct 1;47(1):128–131. doi: 10.1016/0014-5793(74)80441-2. [DOI] [PubMed] [Google Scholar]
  52. Kirk C. J. Ligand-stimulated inositol lipid metabolism in the liver: relationship to receptor function. Cell Calcium. 1982 Oct;3(4-5):399–411. doi: 10.1016/0143-4160(82)90026-4. [DOI] [PubMed] [Google Scholar]
  53. Kirk C. J., Michell R. H., Hems D. A. Phosphatidylinositol metabolism in rat hepatocytes stimulated by vasopressin. Biochem J. 1981 Jan 15;194(1):155–165. doi: 10.1042/bj1940155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  54. Kirk C. J., Rodrigues L. M., Hems D. A. The influence of vasopressin and related peptides on glycogen phosphorylase activity and phosphatidylinositol metabolism in hepatocytes. Biochem J. 1979 Feb 15;178(2):493–496. doi: 10.1042/bj1780493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  55. Kirk C. J., Verrinder T. R., Hems D. A. The influence of extracellular calcium concentration on the vasopressin-stimulated incorporation of inorganic phosphate into phosphatidylinositol in hepatocyte suspensions. Biochem Soc Trans. 1978;6(5):1031–1033. doi: 10.1042/bst0061031. [DOI] [PubMed] [Google Scholar]
  56. Kruszynski M., Lammek B., Manning M., Seto J., Haldar J., Sawyer W. H. [1-beta-Mercapto-beta,beta-cyclopentamethylenepropionic acid),2-(O-methyl)tyrosine ]argine-vasopressin and [1-beta-mercapto-beta,beta-cyclopentamethylenepropionic acid)]argine-vasopressine, two highly potent antagonists of the vasopressor response to arginine-vasopressin. J Med Chem. 1980 Apr;23(4):364–368. doi: 10.1021/jm00178a003. [DOI] [PubMed] [Google Scholar]
  57. Lang V., Pryhitka G., Buckley J. T. Effect of neomycin and ionophore A23189 on ATP levels and turnover of polyphosphoinositides in human erythrocytes. Can J Biochem. 1977 Sep;55(9):1007–1012. doi: 10.1139/o77-150. [DOI] [PubMed] [Google Scholar]
  58. Lin S. H., Fain J. N. Vasopressin and epinephrine stimulation of phosphatidylinositol breakdown in the plasma membrane of rat hepatocytes. Life Sci. 1981 Nov 2;29(18):1905–1912. doi: 10.1016/0024-3205(81)90523-3. [DOI] [PubMed] [Google Scholar]
  59. Mellion B. T., Ignarro L. J., Ohlstein E. H., Pontecorvo E. G., Hyman A. L., Kadowitz P. J. Evidence for the inhibitory role of guanosine 3', 5'-monophosphate in ADP-induced human platelet aggregation in the presence of nitric oxide and related vasodilators. Blood. 1981 May;57(5):946–955. [PubMed] [Google Scholar]
  60. Michell R. H., Harwood J. L., Coleman R., Hawthorne J. N. Characteristics of rat liver phosphatidylinositol kinase and its presence in the plasma membrane. Biochim Biophys Acta. 1967 Dec 5;144(3):649–658. doi: 10.1016/0005-2760(67)90053-7. [DOI] [PubMed] [Google Scholar]
  61. Michell R. H., Hawthorne J. N., Coleman R., Karnovsky M. L. Extraction of polyphosphoinositides with neutral and acidified solvents. A comparison of guinea-pig brain and liver, and measurements of rat liver inositol compounds which are resistant to extraction. Biochim Biophys Acta. 1970 Jun 9;210(1):86–91. doi: 10.1016/0005-2760(70)90064-0. [DOI] [PubMed] [Google Scholar]
  62. Michell R. H., Hawthorne J. N. The site of diphosphoinositide synthesis in rat liver. Biochem Biophys Res Commun. 1965 Nov 22;21(4):333–338. doi: 10.1016/0006-291x(65)90198-1. [DOI] [PubMed] [Google Scholar]
  63. Michell R. H. Inositol phospholipids and cell surface receptor function. Biochim Biophys Acta. 1975 Mar 25;415(1):81–47. doi: 10.1016/0304-4157(75)90017-9. [DOI] [PubMed] [Google Scholar]
  64. Michell R. H. Is phosphatidylinositol really out of the calcium gate? Nature. 1982 Apr 8;296(5857):492–493. doi: 10.1038/296492a0. [DOI] [PubMed] [Google Scholar]
  65. Michell R. H., Jones L. M. Enhanced phosphatidylinositol labelling in rat parotid fragments exposed to alpha-adrenergic stimulation. Biochem J. 1974 Jan;138(1):47–52. doi: 10.1042/bj1380047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  66. Michell R. H., Kirk C. J., Jones L. M., Downes C. P., Creba J. A. The stimulation of inositol lipid metabolism that accompanies calcium mobilization in stimulated cells: defined characteristics and unanswered questions. Philos Trans R Soc Lond B Biol Sci. 1981 Dec 18;296(1080):123–138. doi: 10.1098/rstb.1981.0177. [DOI] [PubMed] [Google Scholar]
  67. Michell R. H., Kirk C. J. Studies of receptor-stimulated inositol lipid metabolism should focus upon measurements of inositol lipid breakdown. Biochem J. 1981 Jul 15;198(1):247–248. doi: 10.1042/bj1980247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  68. Michell R. H. Stimulated inositol lipid metabolism: an introduction. Cell Calcium. 1982 Oct;3(4-5):285–294. doi: 10.1016/0143-4160(82)90017-3. [DOI] [PubMed] [Google Scholar]
  69. Poggioli J., Berthon B., Claret M. Calcium movements in in situ mitochondria following activation of alpha-adrenergic receptors in rat liver cells. FEBS Lett. 1980 Jun 30;115(2):243–246. doi: 10.1016/0014-5793(80)81178-1. [DOI] [PubMed] [Google Scholar]
  70. Putney J. W., Jr Inositol lipids and cell stimulation in mammalian salivary gland. Cell Calcium. 1982 Oct;3(4-5):369–383. doi: 10.1016/0143-4160(82)90024-0. [DOI] [PubMed] [Google Scholar]
  71. Putney J. W., Jr, Poggioli J., Weiss S. J. Receptor regulation of calcium release and calcium permeability in parotid gland cells. Philos Trans R Soc Lond B Biol Sci. 1981 Dec 18;296(1080):37–45. doi: 10.1098/rstb.1981.0169. [DOI] [PubMed] [Google Scholar]
  72. Putney J. W., Jr Recent hypotheses regarding the phosphatidylinositol effect. Life Sci. 1981 Sep 21;29(12):1183–1194. doi: 10.1016/0024-3205(81)90221-6. [DOI] [PubMed] [Google Scholar]
  73. Putney J. W., Jr Stimulus-permeability coupling: role of calcium in the receptor regulation of membrane permeability. Pharmacol Rev. 1978 Jun;30(2):209–245. [PubMed] [Google Scholar]
  74. Reinhart P. H., Taylor W. M., Bygrave F. L. Calcium ion fluxes induced by the action of alpha-adrenergic agonists in perfused rat liver. Biochem J. 1982 Dec 15;208(3):619–630. doi: 10.1042/bj2080619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  75. Roach P. D., Palmer F. B. Human erythrocyte cytosol phosphatidyl-inositol-bisphosphate phosphatase. Biochim Biophys Acta. 1981 Oct 13;661(2):323–333. doi: 10.1016/0005-2744(81)90021-8. [DOI] [PubMed] [Google Scholar]
  76. SANTIAGO-CALVO E., MULE S., REDMAN C. M., HOKIN M. R., HOKIN L. E. THE CHROMATOGRAPHIC SEPARATION OF POLYPHOSPHOINOSITIDES AND STUDIES ON THEIR TURNOVER IN VARIOUS TISSUES. Biochim Biophys Acta. 1964 Oct 2;84:550–562. doi: 10.1016/0926-6542(64)90125-8. [DOI] [PubMed] [Google Scholar]
  77. Schultz K., Schultz K., Schultz G. Sodium nitroprusside and other smooth muscle-relaxants increase cyclic GMP levels in rat ductus deferens. Nature. 1977 Feb 24;265(5596):750–751. doi: 10.1038/265750a0. [DOI] [PubMed] [Google Scholar]
  78. Schulz I., Kimura T., Wakasugi H., Haase W., Kribben A. Analysis of Ca2+ fluxes and Ca2+ pools in pancreatic acini. Philos Trans R Soc Lond B Biol Sci. 1981 Dec 18;296(1080):105–113. doi: 10.1098/rstb.1981.0175. [DOI] [PubMed] [Google Scholar]
  79. Shukla S. D. Minireview. Phosphatidylinositol specific phospholipases C. Life Sci. 1982 Apr 19;30(16):1323–1335. doi: 10.1016/0024-3205(82)90016-9. [DOI] [PubMed] [Google Scholar]
  80. Soukup J., Schanberg S. Involvement of alpha noradrenergic receptors in mediation of brain polyphosphoinositides metabolism in vivo. J Pharmacol Exp Ther. 1982 Jul;222(1):209–214. [PubMed] [Google Scholar]
  81. Takai Y., Kaibuchi K., Matsubara T., Nishizuka Y. Inhibitory action of guanosine 3', 5'-monophosphate on thrombin-induced phosphatidylinositol turnover and protein phosphorylation in human platelets. Biochem Biophys Res Commun. 1981 Jul 16;101(1):61–67. doi: 10.1016/s0006-291x(81)80010-1. [DOI] [PubMed] [Google Scholar]
  82. Takenawa T., Homma Y., Nagai Y. Increased formation of phosphatidic acid induced with vasopressin or Ca2+ ionophore A23187 in rat hepatocytes. Biochem Pharmacol. 1982 Aug 15;31(16):2663–2667. doi: 10.1016/0006-2952(82)90715-8. [DOI] [PubMed] [Google Scholar]
  83. Takhar A. P., Kirk C. J. Stimulation of inorganic-phosphate incorporation into phosphatidylinositol in rat thoracic aorta mediated through V1-vasopressin receptors. Biochem J. 1981 Jan 15;194(1):167–172. doi: 10.1042/bj1940167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  84. Tolbert M. E., White A. C., Aspry K., Cutts J., Fain J. N. Stimulation by vasopressin and alpha-catecholamines of phosphatidylinositol formation in isolated rat liver parenchymal cells. J Biol Chem. 1980 Mar 10;255(5):1938–1944. [PubMed] [Google Scholar]
  85. Wakelam M. J., Walker D. G. De novo synthesis of glucokinase in hepatocytes isolated from neonatal rats. FEBS Lett. 1980 Feb 25;111(1):115–119. doi: 10.1016/0014-5793(80)80774-5. [DOI] [PubMed] [Google Scholar]
  86. Weiss S. J., McKinney J. S., Putney J. W., Jr Receptor-mediated net breakdown of phosphatidylinositol 4,5-bisphosphate in parotid acinar cells. Biochem J. 1982 Sep 15;206(3):555–560. doi: 10.1042/bj2060555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  87. Whitton P. D., Rodrigues L. M., Hems D. A. Influence of extracellular calcium ions on hormonal stimulation of glycogen breakdown in hepatocyte suspensions [proceedings]. Biochem Soc Trans. 1977;5(4):992–994. doi: 10.1042/bst0050992. [DOI] [PubMed] [Google Scholar]
  88. Williamson J. R., Cooper R. H., Hoek J. B. Role of calcium in the hormonal regulation of liver metabolism. Biochim Biophys Acta. 1981 Dec 30;639(3-4):243–295. doi: 10.1016/0304-4173(81)90012-4. [DOI] [PubMed] [Google Scholar]
  89. Yagihara Y., Bleasdale J. E., Hawthorne J. N. Effects of acetylcholine on the incorporation of (32P)orthophosphate in vitro into the phospholipids of subsynaptosomal, membranes from guinea-pig brain. J Neurochem. 1973 Jul;21(1):173–190. doi: 10.1111/j.1471-4159.1973.tb04237.x. [DOI] [PubMed] [Google Scholar]