Studies on heterogeneity of human gastric zymogens (original) (raw)

Abstract

The peptic zymogens of human gastric mucosa have been studied in the past by three different techniques: phosphate-gradient chromatography on a column of DEAE-cellulose, chloride-gradient chromatography on DEAE-cellulose and agar-gel electrophoresis. These techniques have given different results, each suggesting the presence of a different number of pepsin zymogens in human gastric mucosa. In the present experiments gastric mucosal homogenates were subjected to analysis by all three techniques. The two major zymogen peaks obtained from phosphate-gradient chromatography were found to be composed of the three fractions found on chloride-gradient chromatography; these fractions in turn were shown to be heterogeneous on agar-gel electrophoresis. The latter technique demonstrated the presence of seven separable enzymically active components in human gastric mucosal homogenates. The relationships of the components separated by the three techniques are discussed.

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Selected References

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  1. Chiang L., Sanchez-Chiang L., Wolf S., Tang J. The separate determination of human pepsin and gastricsin. Proc Soc Exp Biol Med. 1966 Jul;122(3):700–704. doi: 10.3181/00379727-122-31231. [DOI] [PubMed] [Google Scholar]
  2. Himmelhoch S. R., Peterson E. A. Experimental problems in the use of commercially prepared DEAE-celluloses for protein chromatography. Anal Biochem. 1966 Dec;17(3):383–389. doi: 10.1016/0003-2697(66)90174-6. [DOI] [PubMed] [Google Scholar]
  3. KUSHNER I., RAPP W., BURTIN P. ELECTROPHORETIC AND IMMUNOCHEMICAL DEMONSTRATION OF THE EXISTENCE OF FOUR HUMAN PEPSINOGENS. J Clin Invest. 1964 Oct;43:1983–1993. doi: 10.1172/JCI105072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lee D., Ryle A. P. Pepsinogen D. A fourth proteolytic zymogen from pig gastric mucosa. Biochem J. 1967 Sep;104(3):735–741. doi: 10.1042/bj1040735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. PERLMANN G. E. Enzymic dephosphorylation of pepsin and pepsinogen. J Gen Physiol. 1958 Jan 20;41(3):441–450. doi: 10.1085/jgp.41.3.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. RICHMOND V., TANG J., WOLF S., TRUCCO R. E., CAPUTTO R. Chromatographic isolation of gastricsin, the proteolytic enzyme from gastric juice with pH optimum 3. 2. Biochim Biophys Acta. 1958 Aug;29(2):453–454. doi: 10.1016/0006-3002(58)90220-8. [DOI] [PubMed] [Google Scholar]
  7. RYLE A. P. PEPSINOGEN B: THE ZYMOGEN OF PEPSIN B. Biochem J. 1965 Jul;96:6–16. doi: 10.1042/bj0960006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. RYLE A. P., PORTER R. R. Parapepsins: two proteolytic enzymes associated with porcine pepsin. Biochem J. 1959 Sep;73:75–86. doi: 10.1042/bj0730075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RYLE A. P. Parapepsinogen II: the zymogen of parapepsin II. Biochem J. 1960 Apr;75:145–150. doi: 10.1042/bj0750145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ryle A. P., Hamilton M. P. Pepsinogen C and pepsin C. Further purification and amino acid composition. Biochem J. 1966 Oct;101(1):176–183. doi: 10.1042/bj1010176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SEIJFFERS M. J., MILLER L. L., SEGAL H. L. PARTIAL CHARACTERIZATION OF HUMAN PEPSIN I, PEPSIN IIA, PEPSIN IIB, AND PEPSIN 3. Biochemistry. 1964 Sep;3:1203–1209. doi: 10.1021/bi00897a003. [DOI] [PubMed] [Google Scholar]
  12. SEIJFFERS M. J., SEGAL H. L., MILLER L. L. SEPARATION OF PEPSIN I, PEPSIN II A, PEPSIN II B, AND PEPSIN III FROM HUMAN GASTRIC MUCOSA. Am J Physiol. 1963 Dec;205:1099–1105. doi: 10.1152/ajplegacy.1963.205.6.1099. [DOI] [PubMed] [Google Scholar]
  13. TANG J., TANG K. I. Purification and properties of a zymogen from human gastric mucosa. J Biol Chem. 1963 Feb;238:606–612. [PubMed] [Google Scholar]
  14. Tang J., Mills J., Chiang L., de Chiang L. Gastric pepsin, mucus and clinical secretory studies. I. Gastric pepsin and pepsin inhibitors. Comparative studies on the structure and specificity of human gastricsin, pepsin and zymogen. Ann N Y Acad Sci. 1967 Jan 26;140(2):688–696. doi: 10.1111/j.1749-6632.1967.tb50994.x. [DOI] [PubMed] [Google Scholar]
  15. Turner M. D., Tuxill J. L., Miller L. L., Segal H. L. Measurement of pepsin I (gastricsin) in human gastric juice. Gastroenterology. 1967 Dec;53(6):905–911. [PubMed] [Google Scholar]