Genetic Evidence That the Sans Fille Locus Is Involved in Drosophila Sex Determination (original) (raw)

Abstract

Females homozygous for sans fille(1621) (= fs(1)1621) have an abnormal germ line. Instead of producing eggs, the germ-line cells proliferate forming ovarian tumors or excessive numbers of nurse cells. The Sex-lethal gene product(s) regulate the branch point of the dosage compensation and sex determination pathways in the soma. The role of Sex-lethal in the germ line is not clear but the germ line of females homozygous for female sterile Sex-lethal alleles or germ-line clones of loss-of-function alleles are characterized by ovarian tumors. Females heterozygous for sans fille(1621) or Sex-lethal are phenotypically wild type with respect to viability and fertility but females trans-heterozygous for sans fille(1621) and Sex-lethal show ovarian tumors, somatic sexual transformations, and greatly reduced viability.

Full Text

The Full Text of this article is available as a PDF (4.0 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachiller D., Sánchez L. Mutations affecting dosage compensation in Drosophila melanogaster: effects in the germline. Dev Biol. 1986 Dec;118(2):379–384. doi: 10.1016/0012-1606(86)90007-2. [DOI] [PubMed] [Google Scholar]
  2. Baker B. S., Belote J. M. Sex determination and dosage compensation in Drosophila melanogaster. Annu Rev Genet. 1983;17:345–393. doi: 10.1146/annurev.ge.17.120183.002021. [DOI] [PubMed] [Google Scholar]
  3. Baker B. S., Ridge K. A. Sex and the single cell. I. On the action of major loci affecting sex determination in Drosophila melanogaster. Genetics. 1980 Feb;94(2):383–423. doi: 10.1093/genetics/94.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Banga S. S., Bloomquist B. T., Brodberg R. K., Pye Q. N., Larrivee D. C., Mason J. M., Boyd J. B., Pak W. L. Cytogenetic characterization of the 4BC region on the X chromosome of Drosophila melanogaster: localization of the mei-9, norpA and omb genes. Chromosoma. 1986;93(4):341–346. doi: 10.1007/BF00327593. [DOI] [PubMed] [Google Scholar]
  5. Belote J. M., Baker B. S. The dual functions of a sex determination gene in Drosophila melanogaster. Dev Biol. 1983 Feb;95(2):512–517. doi: 10.1016/0012-1606(83)90054-4. [DOI] [PubMed] [Google Scholar]
  6. Belote J. M., McKeown M. B., Andrew D. J., Scott T. N., Wolfner M. F., Baker B. S. Control of sexual differentiation in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1985;50:605–614. doi: 10.1101/sqb.1985.050.01.073. [DOI] [PubMed] [Google Scholar]
  7. Boggs R. T., Gregor P., Idriss S., Belote J. M., McKeown M. Regulation of sexual differentiation in D. melanogaster via alternative splicing of RNA from the transformer gene. Cell. 1987 Aug 28;50(5):739–747. doi: 10.1016/0092-8674(87)90332-1. [DOI] [PubMed] [Google Scholar]
  8. Bridges C. B. TRIPLOID INTERSEXES IN DROSOPHILA MELANOGASTER. Science. 1921 Sep 16;54(1394):252–254. doi: 10.1126/science.54.1394.252. [DOI] [PubMed] [Google Scholar]
  9. Busson D., Gans M., Komitopoulou K., Masson M. Genetic Analysis of Three Dominant Female-Sterile Mutations Located on the X Chromosome of DROSOPHILA MELANOGASTER. Genetics. 1983 Oct;105(2):309–325. doi: 10.1093/genetics/105.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cline T. W. A female-specific lethal lesion in an X-linked positive regulator of the Drosophila sex determination gene, Sex-lethal. Genetics. 1986 Jul;113(3):641–663. doi: 10.1093/genetics/113.3.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cline T. W. Autoregulatory functioning of a Drosophila gene product that establish es and maintains the sexually determined state. Genetics. 1984 Jun;107(2):231–277. doi: 10.1093/genetics/107.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Cline T. W. Maternal and zygotic sex-specific gene interactions in Drosophila melanogaster. Genetics. 1980 Dec;96(4):903–926. doi: 10.1093/genetics/96.4.903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Cronmiller C., Cline T. W. The Drosophila sex determination gene daughterless has different functions in the germ line versus the soma. Cell. 1987 Feb 13;48(3):479–487. doi: 10.1016/0092-8674(87)90198-x. [DOI] [PubMed] [Google Scholar]
  14. Eberl D. F., Hilliker A. J. Characterization of X-linked recessive lethal mutations affecting embryonic morphogenesis in Drosophila melanogaster. Genetics. 1988 Jan;118(1):109–120. doi: 10.1093/genetics/118.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ferguson E. L., Sternberg P. W., Horvitz H. R. A genetic pathway for the specification of the vulval cell lineages of Caenorhabditis elegans. Nature. 1987 Mar 19;326(6110):259–267. doi: 10.1038/326259a0. [DOI] [PubMed] [Google Scholar]
  16. Gans M., Audit C., Masson M. Isolation and characterization of sex-linked female-sterile mutants in Drosophila melanogaster. Genetics. 1975 Dec;81(4):683–704. doi: 10.1093/genetics/81.4.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Komitopoulou K., Gans M., Margaritis L. H., Kafatos F. C., Masson M. Isolation and Characterization of Sex-Linked Female-Sterile Mutants in DROSOPHILA MELANOGASTER with Special Attention to Eggshell Mutants. Genetics. 1983 Dec;105(4):897–920. doi: 10.1093/genetics/105.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lohs-Schardin M., Sander K., Cremer C., Cremer T., Zorn C. Localized ultraviolet laser microbeam irradiation of early Drosophila embryos: fate maps based on location and frequency of adult defects. Dev Biol. 1979 Feb;68(2):533–545. doi: 10.1016/0012-1606(79)90224-0. [DOI] [PubMed] [Google Scholar]
  19. Lucchesi J. C., Manning J. E. Gene dosage compensation in Drosophila melanogaster. Adv Genet. 1987;24:371–429. doi: 10.1016/s0065-2660(08)60013-9. [DOI] [PubMed] [Google Scholar]
  20. Lucchesi J. C., Skripsky T. The link between dosage compensation and sex differentiation in Drosophila melanogaster. Chromosoma. 1981;82(2):217–227. doi: 10.1007/BF00286106. [DOI] [PubMed] [Google Scholar]
  21. Maine E. M., Salz H. K., Schedl P., Cline T. W. Sex-lethal, a link between sex determination and sexual differentiation in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1985;50:595–604. doi: 10.1101/sqb.1985.050.01.072. [DOI] [PubMed] [Google Scholar]
  22. Marsh J. L., Wieschaus E. Is sex determination in germ line and soma controlled by separate genetic mechanisms? Nature. 1978 Mar 16;272(5650):249–251. doi: 10.1038/272249a0. [DOI] [PubMed] [Google Scholar]
  23. Mohler J. D. Developmental genetics of the Drosophila egg. I. Identification of 59 sex-linked cistrons with maternal effects on embryonic development. Genetics. 1977 Feb;85(2):259–272. doi: 10.1093/genetics/85.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Oliver B., Perrimon N., Mahowald A. P. The ovo locus is required for sex-specific germ line maintenance in Drosophila. Genes Dev. 1987 Nov;1(9):913–923. doi: 10.1101/gad.1.9.913. [DOI] [PubMed] [Google Scholar]
  25. Perrimon N., Gans M. Clonal analysis of the tissue specificity of recessive female-sterile mutations of Drosophila melanogaster using a dominant female-sterile mutation Fs(1)K1237. Dev Biol. 1983 Dec;100(2):365–373. doi: 10.1016/0012-1606(83)90231-2. [DOI] [PubMed] [Google Scholar]
  26. Perrimon N., Mohler D., Engstrom L., Mahowald A. P. X-linked female-sterile loci in Drosophila melanogaster. Genetics. 1986 Jul;113(3):695–712. doi: 10.1093/genetics/113.3.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Salz H. K., Cline T. W., Schedl P. Functional changes associated with structural alterations induced by mobilization of a P element inserted in the Sex-lethal gene of Drosophila. Genetics. 1987 Oct;117(2):221–231. doi: 10.1093/genetics/117.2.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schüpbach T. Normal female germ cell differentiation requires the female X chromosome to autosome ratio and expression of sex-lethal in Drosophila melanogaster. Genetics. 1985 Mar;109(3):529–548. doi: 10.1093/genetics/109.3.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Skripsky T., Lucchesi J. C. Intersexuality resulting from the interaction of sex-specific lethal mutations in Drosophila melanogaster. Dev Biol. 1982 Nov;94(1):153–162. doi: 10.1016/0012-1606(82)90078-1. [DOI] [PubMed] [Google Scholar]
  30. Van Deusen E. B. Sex determination in germ line chimeras of Drosophila melanogaster. J Embryol Exp Morphol. 1977 Feb;37(1):173–185. [PubMed] [Google Scholar]
  31. Wieschaus E., Audit C., Masson M. A clonal analysis of the roles of somatic cells and germ line during oogenesis in Drosophila. Dev Biol. 1981 Nov;88(1):92–103. doi: 10.1016/0012-1606(81)90221-9. [DOI] [PubMed] [Google Scholar]