Transient, Meiosis-Induced Expression of the Rec6 and Rec12 Genes of Schizosaccharomyces Pombe (original) (raw)

Abstract

Two meiotic recombination genes, rec6 and rec12, from Schizosaccharomyces pombe have been cloned by genetic complementation and their DNA sequences determined. Gene replacements demonstrated that the cloned fragments contained the rec6 and rec12 genes. Further analysis showed that the functional rec6 gene was within a 1.3-kb fragment and rec12 within a 1.7-kb fragment. The nucleotide sequences of these fragments revealed open reading frames (ORFs) predicting 143 amino acids for the rec6 gene product and 139 amino acids for the rec12 gene product. After pat1-114 temperature-induced meiosis, the transcripts of rec6 and rec12 were induced to maximal levels at 2-3 hr, at about the time of premeiotic DNA synthesis, but were present at much lower levels before and after this time. The transient induction of the transcripts and the phenotypes of the mutants suggest that the rec6 and rec12 gene products are involved primarily in the early steps of meiotic recombination in S. pombe. Near the rec6 gene are two additional ORFs potentially encoding proteins with homology to ribosomal protein S7 of Saccharomyces cerevisiae (ORF137) and to the homeodomain of developmental regulatory proteins (ORF201). The roles of these S. pombe ORFs remain to be determined.

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Selected References

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  1. Bähler J., Schuchert P., Grimm C., Kohli J. Synchronized meiosis and recombination in fission yeast: observations with pat1-114 diploid cells. Curr Genet. 1991 Jun;19(6):445–451. doi: 10.1007/BF00312735. [DOI] [PubMed] [Google Scholar]
  2. Cottarel G., Beach D., Deuschle U. Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr Genet. 1993 May-Jun;23(5-6):547–548. doi: 10.1007/BF00312650. [DOI] [PubMed] [Google Scholar]
  3. De Veaux L. C., Hoagland N. A., Smith G. R. Seventeen complementation groups of mutations decreasing meiotic recombination in Schizosaccharomyces pombe. Genetics. 1992 Feb;130(2):251–262. doi: 10.1093/genetics/130.2.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DeVeaux L. C., Smith G. R. Region-specific activators of meiotic recombination in Schizosaccharomyces pombe. Genes Dev. 1994 Jan;8(2):203–210. doi: 10.1101/gad.8.2.203. [DOI] [PubMed] [Google Scholar]
  5. Elliott S., Chang C. W., Schweingruber M. E., Schaller J., Rickli E. E., Carbon J. Isolation and characterization of the structural gene for secreted acid phosphatase from Schizosaccharomyces pombe. J Biol Chem. 1986 Feb 25;261(6):2936–2941. [PubMed] [Google Scholar]
  6. Gehring W. J. The homeobox in perspective. Trends Biochem Sci. 1992 Aug;17(8):277–280. doi: 10.1016/0968-0004(92)90434-b. [DOI] [PubMed] [Google Scholar]
  7. Gordon C. B., Campbell J. L. A cell cycle-responsive transcriptional control element and a negative control element in the gene encoding DNA polymerase alpha in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6058–6062. doi: 10.1073/pnas.88.14.6058. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grimm C., Kohli J., Murray J., Maundrell K. Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker. Mol Gen Genet. 1988 Dec;215(1):81–86. doi: 10.1007/BF00331307. [DOI] [PubMed] [Google Scholar]
  9. Gutz H. Site Specific Induction of Gene Conversion in SCHIZOSACCHAROMYCES POMBE. Genetics. 1971 Nov;69(3):317–337. doi: 10.1093/genetics/69.3.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Henikoff S., Henikoff J. G. Automated assembly of protein blocks for database searching. Nucleic Acids Res. 1991 Dec 11;19(23):6565–6572. doi: 10.1093/nar/19.23.6565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
  12. Ito H., Fukuda Y., Murata K., Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983 Jan;153(1):163–168. doi: 10.1128/jb.153.1.163-168.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kelly M., Burke J., Smith M., Klar A., Beach D. Four mating-type genes control sexual differentiation in the fission yeast. EMBO J. 1988 May;7(5):1537–1547. doi: 10.1002/j.1460-2075.1988.tb02973.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kohli J. Genetic nomenclature and gene list of the fission yeast Schizosaccharomyces pombe. Curr Genet. 1987;11(8):575–589. doi: 10.1007/BF00393919. [DOI] [PubMed] [Google Scholar]
  15. Lin Y., Larson K. L., Dorer R., Smith G. R. Meiotically induced rec7 and rec8 genes of Schizosaccharomyces pombe. Genetics. 1992 Sep;132(1):75–85. doi: 10.1093/genetics/132.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lowndes N. F., Johnson A. L., Johnston L. H. Coordination of expression of DNA synthesis genes in budding yeast by a cell-cycle regulated trans factor. Nature. 1991 Mar 21;350(6315):247–250. doi: 10.1038/350247a0. [DOI] [PubMed] [Google Scholar]
  17. Lowndes N. F., McInerny C. J., Johnson A. L., Fantes P. A., Johnston L. H. Control of DNA synthesis genes in fission yeast by the cell-cycle gene cdc10+. Nature. 1992 Jan 30;355(6359):449–453. doi: 10.1038/355449a0. [DOI] [PubMed] [Google Scholar]
  18. Nasmyth K. A., Tatchell K., Hall B. D., Astell C., Smith M. A position effect in the control of transcription at yeast mating type loci. Nature. 1981 Jan 22;289(5795):244–250. doi: 10.1038/289244a0. [DOI] [PubMed] [Google Scholar]
  19. Ponticelli A. S., Smith G. R. Meiotic recombination-deficient mutants of Schizosaccharomyces pombe. Genetics. 1989 Sep;123(1):45–54. doi: 10.1093/genetics/123.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Russell P. R., Hall B. D. Structure of the Schizosaccharomyces pombe cytochrome c gene. Mol Cell Biol. 1982 Feb;2(2):106–116. doi: 10.1128/mcb.2.2.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Scott M. P., Tamkun J. W., Hartzell G. W., 3rd The structure and function of the homeodomain. Biochim Biophys Acta. 1989 Jul 28;989(1):25–48. doi: 10.1016/0304-419x(89)90033-4. [DOI] [PubMed] [Google Scholar]
  22. Smith G. R. Homologous recombination in procaryotes. Microbiol Rev. 1988 Mar;52(1):1–28. doi: 10.1128/mr.52.1.1-28.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Synetos D., Dabeva M. D., Warner J. R. The yeast ribosomal protein S7 and its genes. J Biol Chem. 1992 Feb 15;267(5):3008–3013. [PubMed] [Google Scholar]
  24. Szankasi P., Smith G. R. A DNA exonuclease induced during meiosis of Schizosaccharomyces pombe. J Biol Chem. 1992 Feb 15;267(5):3014–3023. [PubMed] [Google Scholar]