Dendritic cells and IFN-alpha-producing cells are two functionally distinct non-B, non-monocytic HLA-DR+ cell subsets in human peripheral blood (original) (raw)

. 1989 Dec;68(4):488–490.

Abstract

At least two distinct HLA-DR+ cell subsets lacking surface markers specific for B cells, monocytes or other known lineages are present in human peripheral blood. One subset is non-adherent to plastic, produces interferon-alpha (IFN-alpha) when incubated with cytomegalovirus-infected target cells and provides an accessory function required for natural killer (NK) cell-mediated lysis of such cells. These non-adherent HLA-DR+ cells express the surface antigen recognized by antibody anti-D44 and do not stimulate mixed leucocyte reaction (MLR). The other HLA-DR+ cell subset is loosely adherent to plastic, produces only minimal levels of IFN-alpha when incubated with cytomegalovirus-infected target cells and does not provide the accessory function required for NK cell-mediated lysis of such cells. These HLA-DR+ cells stimulate a strong MLR, do not express D44 antigen and meet the criteria of dendritic cells (DC) morphologically and functionally.

486

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bandyopadhyay S., Oh S. H., Michelson S., Miller D. S., Virelizier J. L., Starr S. E. Natural killing of fibroblasts infected with low-passage clinical isolates of human cytomegalovirus. Clin Exp Immunol. 1988 Jul;73(1):11–16. [PMC free article] [PubMed] [Google Scholar]
  2. Bandyopadhyay S., Perussia B., Trinchieri G., Miller D. S., Starr S. E. Requirement for HLA-DR+ accessory cells in natural killing of cytomegalovirus-infected fibroblasts. J Exp Med. 1986 Jul 1;164(1):180–195. doi: 10.1084/jem.164.1.180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bernard A., Gay-Bellile V., Amiot M., Caillou B., Charbord P., Boumsell L. A novel human leukocyte differentiation antigen: monoclonal antibody anti-D44 defines a 28 Kd molecule present on immature hematologic cells and a subpopulation of mature T cells. J Immunol. 1984 May;132(5):2338–2344. [PubMed] [Google Scholar]
  4. Dayton E. T., Perussia B., Trinchieri G. Correlation between differentiation, expression of monocyte-specific antigens, and cytotoxic functions in human promyelocytic cell lines treated with leukocyte-conditioned medium. J Immunol. 1983 Mar;130(3):1120–1128. [PubMed] [Google Scholar]
  5. Fitzgerald-Bocarsly P., Feldman M., Mendelsohn M., Curl S., Lopez C. Human mononuclear cells which produce interferon-alpha during NK(HSV-FS) assays are HLA-DR positive cells distinct from cytolytic natural killer effectors. J Leukoc Biol. 1988 Apr;43(4):323–334. doi: 10.1002/jlb.43.4.323. [DOI] [PubMed] [Google Scholar]
  6. Hart D. N., McKenzie J. L. Isolation and characterization of human tonsil dendritic cells. J Exp Med. 1988 Jul 1;168(1):157–170. doi: 10.1084/jem.168.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Inaba K., Steinman R. M., Van Voorhis W. C., Muramatsu S. Dendritic cells are critical accessory cells for thymus-dependent antibody responses in mouse and in man. Proc Natl Acad Sci U S A. 1983 Oct;80(19):6041–6045. doi: 10.1073/pnas.80.19.6041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Inaba K., Witmer M. D., Steinman R. M. Clustering of dendritic cells, helper T lymphocytes, and histocompatible B cells during primary antibody responses in vitro. J Exp Med. 1984 Sep 1;160(3):858–876. doi: 10.1084/jem.160.3.858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klinkert W. E., LaBadie J. H., O'Brien J. P., Beyer C. F., Bowers W. E. Rat dendritic cells function as accessory cells and control the production of a soluble factor required for mitogenic responses of T lymphocytes. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5414–5418. doi: 10.1073/pnas.77.9.5414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Knight S. C., Farrant J., Bryant A., Edwards A. J., Burman S., Lever A., Clarke J., Webster A. D. Non-adherent, low-density cells from human peripheral blood contain dendritic cells and monocytes, both with veiled morphology. Immunology. 1986 Apr;57(4):595–603. [PMC free article] [PubMed] [Google Scholar]
  11. Kung P., Goldstein G., Reinherz E. L., Schlossman S. F. Monoclonal antibodies defining distinctive human T cell surface antigens. Science. 1979 Oct 19;206(4416):347–349. doi: 10.1126/science.314668. [DOI] [PubMed] [Google Scholar]
  12. Lebon P., Bernard A., Boumsell L. Identification de populations lymphocytaires productrices d'interféron-alpha par des anticorps monoclonaux. C R Seances Acad Sci III. 1982 Sep 20;295(2):79–82. [PubMed] [Google Scholar]
  13. Oh S. H., Bandyopadhyay S., Miller D. S., Starr S. E. Cooperation between CD16(Leu-11b)+ NK cells and HLA-DR+ cells in natural killing of herpesvirus-infected fibroblasts. J Immunol. 1987 Oct 15;139(8):2799–2802. [PubMed] [Google Scholar]
  14. Perussia B., Fanning V., Trinchieri G. A leukocyte subset bearing HLA-DR antigens is responsible for in vitro alpha interferon production in response to viruses. Nat Immun Cell Growth Regul. 1985;4(3):120–137. [PubMed] [Google Scholar]
  15. Perussia B., Trinchieri G., Jackson A., Warner N. L., Faust J., Rumpold H., Kraft D., Lanier L. L. The Fc receptor for IgG on human natural killer cells: phenotypic, functional, and comparative studies with monoclonal antibodies. J Immunol. 1984 Jul;133(1):180–189. [PubMed] [Google Scholar]
  16. Perussia B., Trinchieri G., Lebman D., Jankiewicz J., Lange B., Rovera G. Monoclonal antibodies that detect differentiation surface antigens on human myelomonocytic cells. Blood. 1982 Feb;59(2):382–392. [PubMed] [Google Scholar]
  17. Steinman R. M., Cohn Z. A. Identification of a novel cell type in peripheral lymphoid organs of mice. I. Morphology, quantitation, tissue distribution. J Exp Med. 1973 May 1;137(5):1142–1162. doi: 10.1084/jem.137.5.1142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Van Voorhis W. C., Hair L. S., Steinman R. M., Kaplan G. Human dendritic cells. Enrichment and characterization from peripheral blood. J Exp Med. 1982 Apr 1;155(4):1172–1187. doi: 10.1084/jem.155.4.1172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Waalen K., Thoen J., Førre O., Hovig T., Teigland J., Natvig J. B. Rheumatoid synovial dendritic cells as stimulators in allogeneic and autologous mixed leukocyte reactions--comparison with autologous monocytes as stimulator cells. Scand J Immunol. 1986 Feb;23(2):233–241. doi: 10.1111/j.1365-3083.1986.tb01962.x. [DOI] [PubMed] [Google Scholar]
  20. Young J. W., Steinman R. M. Accessory cell requirements for the mixed-leukocyte reaction and polyclonal mitogens, as studied with a new technique for enriching blood dendritic cells. Cell Immunol. 1988 Jan;111(1):167–182. doi: 10.1016/0008-8749(88)90061-5. [DOI] [PubMed] [Google Scholar]