Reagin-like antibodies in rats infected with the nematode parasite Nippostrongylus brasiliensis (original) (raw)

. 1967 Feb;12(2):113–131.

Abstract

The immune response to Nippostrongylus brasiliensis in rats is closely associated with the formation of antibodies resembling human reagins. These are detected in some rats immediately after acquiring resistance to the parasite after an initial infection and in all rats 1 week later. Further infections stimulate an anamnestic rise in `reagin' production. `Reagin' formation is due to the living worms, primarily the adult stage. `Reagins' were detected by passive cutaneous anaphylaxis in the rat (homologous passive cutaneous anaphylaxis=PCA) with a 72-hour interval between intradermal injection of antibody and intravenous injection of antigen, which was a saline extract of adult worms.

`Reagin' production in rats vaccinated with worm extracts contrasts sharply with `reagin' production in rats infected with living worms. Vaccination with worm extracts stimulates `reagin' production in only some rats during the primary response only; after second and later vaccinations, `reagins' were not detected in any rats. Prolonged vaccination induces the formation of `blocking' antibodies and a further type of antibody capable of inducing 6-hour PCA, but not 72-hour PCA in rats. Even after prolonged vaccination, however, a subsequent infection stimulates an anamnestic rise in `reagin' production in some rats. Vaccination did not induce resistance to infection in any rats.

Rats recovered from an initial infection become increasingly susceptible to anaphylactic shock when challenged with worm antigen, but the sensitivity of rats to systemic anaphylaxis is not closely correlated to the level of circulating `reagins'. Heterologous anaphylactic reactions did not induce expulsion of worms from the intestine and anaphylaxis is not directly related to the ability of rats to resist infection.

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Selected References

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  1. BINAGHI R. A., BENACERRAF B., BLOCH K. J., KOURILSKY F. M. PROPERTIES OF RAT ANAPHYLACTIC ANTIBODY. J Immunol. 1964 Jun;92:927–933. [PubMed] [Google Scholar]
  2. BINAGHI R. A., BENACERRAF B. THE PRODUCTION OF ANAPHYLACTIC ANTIBODY IN THE RAT. J Immunol. 1964 Jun;92:920–926. [PubMed] [Google Scholar]
  3. CRABBE P. A., CARBONARA A. O., HEREMANS J. F. THE NORMAL HUMAN INTESTINAL MUCOSA AS A MAJOR SOURCE OF PLASMA CELLS CONTAINING GAMMA-A-IMMUNOGLOBULIN. Lab Invest. 1965 Mar;14:235–248. [PubMed] [Google Scholar]
  4. FIREMAN P., VANNIER W. E., GOODMAN H. C. The association of skin-sensitizing antibody with the beta 2A-globulins in sera from ragweed-sensitive patients. J Exp Med. 1963 Apr 1;117:603–620. doi: 10.1084/jem.117.4.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HEREMANS J. F., VAERMAN J. P. Beta-2A-Globulin as a possible carrier of allergic reaginic activity. Nature. 1962 Mar 17;193:1091–1092. doi: 10.1038/1931091b0. [DOI] [PubMed] [Google Scholar]
  6. MOTA I. PASSIVE CUTANEOUS ANAPHYLAXIS INDUCED WITH MAST CELL-SENSITIZING ANTIBODY. THE ROLE OF HISTAMINE AND 5-HYDROXYTRYPTAMINE. Life Sci. 1963 Dec;12:917–927. doi: 10.1016/0024-3205(63)90060-2. [DOI] [PubMed] [Google Scholar]
  7. MOTA I. THE MECHANISM OF ANAPHYLAXIS. I. PRODUCTION AND BIOLOGICAL PROPERTIES OF 'MAST CELL SENSITIZING' ANTIBODY. Immunology. 1964 Nov;7:681–699. [PMC free article] [PubMed] [Google Scholar]
  8. OGILVIE B. M. REAGIN-LIKE ANTIBODIES IN ANIMALS IMMUNE TO HELMINTH PARASITES. Nature. 1964 Oct 3;204:91–92. doi: 10.1038/204091a0. [DOI] [PubMed] [Google Scholar]
  9. OGILVIE B. M. ROLE OF ADULT WORMS IN IMMUNITY OF RATS TO NIPPOSTRONGYLUS BRASILIENSIS. Parasitology. 1965 May;55:325–335. doi: 10.1017/s0031182000068797. [DOI] [PubMed] [Google Scholar]
  10. Ogilvie B. M., Smithers S. R., Terry R. J. Reagin-like antibodies in experimental infections of Schistosoma mansoni and the passive transfer of resistance. Nature. 1966 Mar 19;209(5029):1221–1223. doi: 10.1038/2091221a0. [DOI] [PubMed] [Google Scholar]
  11. SANYAL R. K., WEST G. B. Anaphylactic shock in the albino rat. J Physiol. 1958 Aug 6;142(3):571–584. doi: 10.1113/jphysiol.1958.sp006037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Terr A. I., Bentz J. D. Skin-sensitizing antibodies in serum sickness. Association of reaginic activity with gamma-2- and gamma-im-globulins. J Allergy. 1965 Sep-Oct;36(5):433–445. doi: 10.1016/0021-8707(65)90137-1. [DOI] [PubMed] [Google Scholar]
  13. Urquhart G. M., Mulligan W., Eadie R. M., Jennings F. W. Immunological studies on Nippostrongylus brasiliensis infection in the rat: the role of local anaphylaxis. Exp Parasitol. 1965 Oct;17(2):210–217. doi: 10.1016/0014-4894(65)90023-8. [DOI] [PubMed] [Google Scholar]