Immune reactions in mucous membranes: I. Intestinal mast cell response during helminth expulsion in the rat (original) (raw)

. 1971 Mar;20(3):277–288.

Abstract

Measurement of the mast cell response in the jejunal mucosae of rats infected with Nippostrongylus brasiliensis was carried out at the time of worm expulsion (self-cure). Just prior to the start of self-cure, a new mast cell population differentiated from cells with large nuclei and prominent nucleoli. Beginning on the 10th day of infection, mast cell numbers increased in an exponential fashion and reached a peak in the middle of the self-cure reaction 14 days after infection. At first the cells had few granules but their granule content subsequently increased and by day 14 some of them appeared to be mature. Mitoses were observed in granulated cells at all stages of the population expansion.

Differential counts showed that an increasing proportion of the mast cell population migrated intraepithelially to become globule leucocytes so that by day 14 the ratio of globule leucocytes to mast cells was 1/1. On subsequent days of infection, this ratio and the total population of granulated cells gradually diminished.

The results suggest that cell differentiation and division are responsible for the population increase. The high globule leucocyte/mast cell ratio points to an extensive release of amines from the granules during self-cure. The significance of this reaction in relation to antibody release from the mucosa and to worm expulsion is discussed.

277

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLOOM G., KELLY J. W. The copper phthalocyanin dye "Astrablau" and its staining properties, especially the staining of mast cells. Z Zellforch Microsk Anat Histochem. 1960;2:48–57. doi: 10.1007/BF00736491. [DOI] [PubMed] [Google Scholar]
  2. GINSBURG H. The in vitro differentiation and culture of normal mast cells from the mouse thymus. Ann N Y Acad Sci. 1963 Feb 26;103:20–39. doi: 10.1111/j.1749-6632.1963.tb53690.x. [DOI] [PubMed] [Google Scholar]
  3. Ginsburg H., Lagunoff D. The in vitro differentiation of mast cells. Cultures of cells from immunized mouse lymph nodes and thoracic duct lymph on fibroblast monolayers. J Cell Biol. 1967 Dec;35(3):685–697. doi: 10.1083/jcb.35.3.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jarrett E. E., Jarrett W. F., Urquhart G. M. Quantitative studies on the kinetics of establishment and expulsion of intestinal nematode populations in susceptible and immune hosts. Nippostrongylus brasiliensis in the rat. Parasitology. 1968 Aug;58(3):625–639. doi: 10.1017/s0031182000028924. [DOI] [PubMed] [Google Scholar]
  5. Jones V. E., Ogilvie B. M. Reaginic antibodies and immunity to Nippostrongylus brasiliensis in the rat. II. Some properties of the antibodies and antigens. Immunology. 1967 May;12(5):583–597. [PMC free article] [PubMed] [Google Scholar]
  6. Miller J. J., 3rd, Cole L. J. Proliferation of mast cells after antigenic stimulation in adult rats. Nature. 1968 Jan 20;217(5125):263–264. doi: 10.1038/217263a0. [DOI] [PubMed] [Google Scholar]
  7. Nordström C., Dahlqvist A., Josefsson L. Quantitative determination of enzymes in different parts of the villi and crypts of rat small intestine. Comparison of alkaline phosphatase, disaccharidases and dipepeptidases. J Histochem Cytochem. 1967 Dec;15(12):713–721. doi: 10.1177/15.12.713. [DOI] [PubMed] [Google Scholar]
  8. OGILVIE B. M. REAGIN-LIKE ANTIBODIES IN ANIMALS IMMUNE TO HELMINTH PARASITES. Nature. 1964 Oct 3;204:91–92. doi: 10.1038/204091a0. [DOI] [PubMed] [Google Scholar]
  9. Ogilvie B. M. Reagin-like antibodies in rats infected with the nematode parasite Nippostrongylus brasiliensis. Immunology. 1967 Feb;12(2):113–131. [PMC free article] [PubMed] [Google Scholar]
  10. Reynolds D. G., Brim J., Sheehy T. W. The vascular architecture of the small intestinal mucosa of the monkey (Macaca mulatta). Anat Rec. 1967 Oct;159(2):211–218. doi: 10.1002/ar.1091590210. [DOI] [PubMed] [Google Scholar]
  11. Urquhart G. M., Mulligan W., Eadie R. M., Jennings F. W. Immunological studies on Nippostrongylus brasiliensis infection in the rat: the role of local anaphylaxis. Exp Parasitol. 1965 Oct;17(2):210–217. doi: 10.1016/0014-4894(65)90023-8. [DOI] [PubMed] [Google Scholar]
  12. Uvnäs B., Wold J. K. Isolation of a mast cell degranulating polypeptide from Ascaris suis. Acta Physiol Scand. 1967 Jul-Aug;70(3):269–276. doi: 10.1111/j.1748-1716.1967.tb03625.x. [DOI] [PubMed] [Google Scholar]
  13. Whur P. Relationship of globule leucocytes to gastrointestinal nematodes in the sheep, and Nippostrongylus brasiliensis and Hymenolepis nana infections in rats. J Comp Pathol. 1966 Jan;76(1):57–65. doi: 10.1016/0021-9975(66)90048-x. [DOI] [PubMed] [Google Scholar]
  14. Wilson R. J., Bloch K. J. Homocytotropic antibody response in the rat infected with the nematode, Nippostrongylus brasiliensis. II. Characteristics of the immune response. J Immunol. 1968 Mar;100(3):622–628. [PubMed] [Google Scholar]
  15. Wilson R. J. Homocytotropic antibody response to the nematode Nippostrongylus brasiliensis in the rat--studies on the worm antigen. J Parasitol. 1967 Aug;53(4):752–762. [PubMed] [Google Scholar]