HMGI-C expression patterns in human tissues. Implications for the genesis of frequent mesenchymal tumors (original) (raw)

. 1996 Sep;149(3):775–779.

Abstract

Cytogenetically visible aberrations of chromosomal region 12q14-15 in a variety of frequent benign human tumors reflect rearrangements of the HMGI-C gene. The mechanisms by which the HMGI-C gene contributes to tumorigenesis are mostly unknown, although frequently aberrant transcripts containing exons 1 to 3 of HMGI-C and ectopic sequences from other genes due to breaks within the third intron of HMGI-C are detectable. This is the first report analyzing human tissue samples mainly of mesenchymal origin by a highly sensitive polymerase-chain-reaction-based approach detecting HMGI-C expression. We found HMGI-C expression in embryonic tissue but no expression in any of several adult tissues tested except for two myometrial tissues. These data suggest that HMGI-C is mainly expressed in human tissues during embryonal and fetal development. Thus, its particular role for tumor development may be due to the expression of at least exons 1 to 3 rather than to the formation of fusion transcripts.

775

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashar H. R., Fejzo M. S., Tkachenko A., Zhou X., Fletcher J. A., Weremowicz S., Morton C. C., Chada K. Disruption of the architectural factor HMGI-C: DNA-binding AT hook motifs fused in lipomas to distinct transcriptional regulatory domains. Cell. 1995 Jul 14;82(1):57–65. doi: 10.1016/0092-8674(95)90052-7. [DOI] [PubMed] [Google Scholar]
  2. Baier G., Telford D., Gulbins E., Yamada N., Kawakami T., Altman A. Improved specificity of RT-PCR amplifications using nested cDNA primers. Nucleic Acids Res. 1993 Mar 11;21(5):1329–1330. doi: 10.1093/nar/21.5.1329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Belge G., Kazmierczak B., Meyer-Bolte K., Bartnitzke S., Bullerdiek J. Expression of SV40 T-antigen in lipoma cells with a chromosomal translocation T(3;12) is not sufficient for direct immortalization. Cell Biol Int Rep. 1992 Apr;16(4):339–347. doi: 10.1016/s0309-1651(06)80139-6. [DOI] [PubMed] [Google Scholar]
  4. Chau K. Y., Patel U. A., Lee K. L., Lam H. Y., Crane-Robinson C. The gene for the human architectural transcription factor HMGI-C consists of five exons each coding for a distinct functional element. Nucleic Acids Res. 1995 Nov 11;23(21):4262–4266. doi: 10.1093/nar/23.21.4262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kazmierczak B., Hennig Y., Wanschura S., Rogalla P., Bartnitzke S., Van de Ven W., Bullerdiek J. Description of a novel fusion transcript between HMGI-C, a gene encoding for a member of the high mobility group proteins, and the mitochondrial aldehyde dehydrogenase gene. Cancer Res. 1995 Dec 15;55(24):6038–6039. [PubMed] [Google Scholar]
  6. Kazmierczak B., Rosigkeit J., Wanschura S., Meyer-Bolte K., Van de Ven W. J., Kayser K., Krieghoff B., Kastendiek H., Bartnitzke S., Bullerdiek J. HMGI-C rearrangements as the molecular basis for the majority of pulmonary chondroid hamartomas: a survey of 30 tumors. Oncogene. 1996 Feb 1;12(3):515–521. [PubMed] [Google Scholar]
  7. Kazmierczak B., Wanschura S., Meyer-Bolte K., Caselitz J., Meister P., Bartnitzke S., Van de Ven W., Bullerdiek J. Cytogenic and molecular analysis of an aggressive angiomyxoma. Am J Pathol. 1995 Sep;147(3):580–585. [PMC free article] [PubMed] [Google Scholar]
  8. Kazmierczak B., Wanschura S., Rosigkeit J., Meyer-Bolte K., Uschinsky K., Haupt R., Schoenmakers E. F., Bartnitzke S., Van de Ven W. J., Bullerdiek J. Molecular characterization of 12q14-15 rearrangements in three pulmonary chondroid hamartomas. Cancer Res. 1995 Jun 15;55(12):2497–2499. [PubMed] [Google Scholar]
  9. Rogalla P., Rohen C., Hennig Y., Deichert U., Bonk U., Bullerdiek J. Telomere repeat fragment sizes do not limit the growth potential of uterine leiomyomas. Biochem Biophys Res Commun. 1995 Jun 6;211(1):175–182. doi: 10.1006/bbrc.1995.1793. [DOI] [PubMed] [Google Scholar]
  10. Rohen C., Caselitz J., Stern C., Wanschura S., Schoenmakers E. F., Van de Ven W. J., Bartnitzke S., Bullerdiek J. A hamartoma of the breast with an aberration of 12q mapped to the MAR region by fluorescence in situ hybridization. Cancer Genet Cytogenet. 1995 Oct 1;84(1):82–84. doi: 10.1016/0165-4608(95)00060-7. [DOI] [PubMed] [Google Scholar]
  11. Schoenmakers E. F., Mols R., Wanschura S., Kools P. F., Geurts J. M., Bartnitzke S., Bullerdiek J., van den Berghe H., Van de Ven W. J. Identification, molecular cloning, and characterization of the chromosome 12 breakpoint cluster region of uterine leiomyomas. Genes Chromosomes Cancer. 1994 Oct;11(2):106–118. doi: 10.1002/gcc.2870110207. [DOI] [PubMed] [Google Scholar]
  12. Schoenmakers E. F., Wanschura S., Mols R., Bullerdiek J., Van den Berghe H., Van de Ven W. J. Recurrent rearrangements in the high mobility group protein gene, HMGI-C, in benign mesenchymal tumours. Nat Genet. 1995 Aug;10(4):436–444. doi: 10.1038/ng0895-436. [DOI] [PubMed] [Google Scholar]
  13. Staats B., Bonk U., Wanschura S., Hanisch P., Schoenmakers E. F., Van de Ven W. J., Bartnitzke S., Bullerdiek J. A fibroadenoma with a t(4;12) (q27;q15) affecting the HMGI-C gene, a member of the high mobility group protein gene family. Breast Cancer Res Treat. 1996;38(3):299–303. doi: 10.1007/BF01806149. [DOI] [PubMed] [Google Scholar]
  14. Van de Ven W. J., Schoenmakers E. F., Wanschura S., Kazmierczak B., Kools P. F., Geurts J. M., Bartnitzke S., Van den Berghe H., Bullerdiek J. Molecular characterization of MAR, a multiple aberration region on human chromosome segment 12q13-q15 implicated in various solid tumors. Genes Chromosomes Cancer. 1995 Apr;12(4):296–303. doi: 10.1002/gcc.2870120410. [DOI] [PubMed] [Google Scholar]
  15. Wanschura S., Kazmierczak B., Schoenmakers E., Meyen E., Bartnitzke S., Van de Ven W., Bullerdiek J., Schloot W. Regional fine mapping of the multiple-aberration region involved in uterine leiomyoma, lipoma, and pleomorphic adenoma of the salivary gland to 12q15. Genes Chromosomes Cancer. 1995 Sep;14(1):68–70. doi: 10.1002/gcc.2870140112. [DOI] [PubMed] [Google Scholar]
  16. Zhou X., Benson K. F., Ashar H. R., Chada K. Mutation responsible for the mouse pygmy phenotype in the developmentally regulated factor HMGI-C. Nature. 1995 Aug 31;376(6543):771–774. doi: 10.1038/376771a0. [DOI] [PubMed] [Google Scholar]