THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES (original) (raw)

Abstract

A clear-cut serological differentiation between AKR lymphocytes of thymic and non-thymic origin is reported: these two cell types are antigenically distinct. In newborn mice, the AKR thymic antigen was found at a high concentration only in thymus. In adult mice, the antigen was present at a high level in thymus, all nervous tissues tested, and some leukemias. It was present at much lower levels in lymph node lymphocytes, splenic lymphocytes, appendix, lung, and certain other leukemias, which appeared to be of non-thymic origin. The AKR thymic antigen was present at a high level in thymus and nervous tissues of RF mice, but was absent from thymocytes of sixteen other mouse strains. These sixteen strains possessed the C3HeB/Fe thymic antigen. The distribution of this antigen in neonatal and adult tissues of the strains tested was similar to that of the AKR thymic antigen in AKR mice. No exceptions were found. These results were obtained by use of immune cytolysis, and of a new method for the quantitative treatment of data from absorption experiments.

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Selected References

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  1. AMOS D. B., ZUMPFT M., ARMSTRONG P. H-5.A AND H-6.A, TWO MOUSE ISOANTIGENS ON RED CELLS AND TISSUES DETECTED SEROLOGICALLY. Transplantation. 1963 Jul;1:270–283. doi: 10.1097/00007890-196301030-00002. [DOI] [PubMed] [Google Scholar]
  2. BOYSE E. A., OLD L. J., LUELL S. GENETIC DETERMINATION OF THE TL (THYMUSLEUKAEMIA) ANTIGEN IN THE MOUSE. Nature. 1964 Feb 22;201:779–779. doi: 10.1038/201779a0. [DOI] [PubMed] [Google Scholar]
  3. BOYSE E. A., OLD L. J., STOCKERT E. Some further data on cytotoxic isoantibodies in the mouse. Ann N Y Acad Sci. 1962 Oct 24;99:574–587. doi: 10.1111/j.1749-6632.1962.tb45339.x. [DOI] [PubMed] [Google Scholar]
  4. Eichwald E. J., Silmser C. R. Skin. Transplant Bull. 1955;2:148–149. [PubMed] [Google Scholar]
  5. GLOBERSON A., FELDMAN M. ROLE OF THE THYMUS IN RESTORATION OF IMMUNE REACTIVITY AND LYMPHOID REGENERATION IN IRRADIATED MICE. Transplantation. 1964 Mar;2:212–227. doi: 10.1097/00007890-196403000-00006. [DOI] [PubMed] [Google Scholar]
  6. GORER P. A., AMOS D. B. Passive immunity in mice against C57BL leukosis E.L. 4 by means of iso-immune serum. Cancer Res. 1956 May;16(4):338–343. [PubMed] [Google Scholar]
  7. LAW L. W., TRAININ N., LEVEY R. H., BARTH W. F. HUMORAL THYMIC FACTOR IN MICE: FURTHER EVIDENCE. Science. 1964 Mar 6;143(3610):1049–1051. doi: 10.1126/science.143.3610.1049. [DOI] [PubMed] [Google Scholar]
  8. OLD L. J., BOYSE E. A. ANTIGENIC PROPERTIES OF EXPERIMENTAL LEUKEMIAS. I. SEROLOGICAL STUDIES IN VITRO WITH SPONTANEOUS AND RADIATION-INDUCED LEUKEMIAS. J Natl Cancer Inst. 1963 Oct;31:977–995. [PubMed] [Google Scholar]
  9. OLD L. J., BOYSE E. A., STOCKERT E. TYPING OF MOUSE LEUKAEMIAS BY SEROLOGICAL METHODS. Nature. 1964 Feb 22;201:777–779. doi: 10.1038/201777a0. [DOI] [PubMed] [Google Scholar]
  10. OSOBA D., MILLER J. F. THE LYMPHOD TISSUES AND IMMUNE RESPONSES OF NEONATALLY THYMECTOMIZED MICE BEARING THYMUS TISSUE IN MILLIPORE DIFFUSION CHAMBERS. J Exp Med. 1964 Jan 1;119:177–194. doi: 10.1084/jem.119.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. REIF A. E., ALLEN J. M. SPECIFICITY OF ISOANTISERA AGAINST LEUKAEMIC AND THYMIC LYMPHOCYTES. Nature. 1963 Dec 28;200:1332–1333. doi: 10.1038/2001332b0. [DOI] [PubMed] [Google Scholar]
  12. REIF A. E. IMMUNE CYTOLYSIS OF MOUSE THYMIC LYMPHOCYTES. J Immunol. 1963 Oct;91:557–567. [PubMed] [Google Scholar]
  13. REIF A. E. Immune cytolysis of three mouse ascites tumors. J Immunol. 1962 Dec;89:849–860. [PubMed] [Google Scholar]
  14. REIF A. E., McVETY L. M., KLEIN E. R. Specificity of antisera to mouse ascites tumor in rabbits immunologically depressed with mouse red cells. J Immunol. 1963 Jan;90:24–28. [PubMed] [Google Scholar]
  15. REIF A. E., NORRIS H. J. A system for quantitative determination of cytotoxic activity of antisera to ascites tumor cells. Cancer Res. 1960 Sep;20:1235–1244. [PubMed] [Google Scholar]
  16. SLETTENMARK B., KLEIN E. Cytotoxic and neutralization tests with serum and lymph node cells of isologous mice with induced resistance against gross lymphomas. Cancer Res. 1962 Sep;22:947–954. [PubMed] [Google Scholar]
  17. WINN H. J. The immune response and the homograft reaction. Natl Cancer Inst Monogr. 1960 Mar;2:113–138. [PubMed] [Google Scholar]