THE FREQUENCY OF ANTIGEN-SENSITIVE CELLS IN TISSUE TRANSPLANTATION: A COMMENTARY ON CLONAL SELECTION (original) (raw)

Abstract

Graft-vs.-host (GVH) reactions were performed in chicken embryos by intravenous injection of adult chicken blood dilutions, and the result was scored by weighing the spleens as well as by karyological identification of host and donor metaphases. From the frequency of detectable signs of GVH reaction when low doses of donor cells were injected into hosts containing one foreign allele of the B locus it is concluded that 1–2% must be a minimum estimate for the frequency of antigen-sensitive cells of a given specificity in this system. It is not found possible to reconcile the findings with the strictest form of clonal selection which postulates a single receptor specificity per clone of antigen-sensitive cells.

Full Text

The Full Text of this article is available as a PDF (499.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boak J. L., Christie G. H., Ford W. L., Howard J. G. Pathways in the development of liver macrophages: alternative precursors contained in populations of lymphocytes and bone-marrow cells. Proc R Soc Lond B Biol Sci. 1968 Feb 27;169(1016):307–327. doi: 10.1098/rspb.1968.0013. [DOI] [PubMed] [Google Scholar]
  2. Brent L., Medawar P. Quantitative studies on tissue transplantation immunity. VII. The normal lymphocyte transfer reaction. Proc R Soc Lond B Biol Sci. 1966 Sep 13;165(1000):281–307. doi: 10.1098/rspb.1966.0069. [DOI] [PubMed] [Google Scholar]
  3. Gowans J. L., Uhr J. W. The carriage of immunological memory by small lymphocytes in the rat. J Exp Med. 1966 Nov 1;124(5):1017–1030. doi: 10.1084/jem.124.5.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hildemann W. H., Pinkerton W. Alloantibody production measured by plaque assay in relation to strong and weak histoincompatibility. J Exp Med. 1966 Nov 1;124(5):885–900. doi: 10.1084/jem.124.5.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MCINTIRE K. R., SELL S., MILLER J. F. PATHOGENESIS OF THE POST-NEONATAL THYMECTOMY WASTING SYNDROME. Nature. 1964 Oct 10;204:151–155. doi: 10.1038/204151a0. [DOI] [PubMed] [Google Scholar]
  6. Miller J. F., Osoba D. Current concepts of the immunological function of the thymus. Physiol Rev. 1967 Jul;47(3):437–520. doi: 10.1152/physrev.1967.47.3.437. [DOI] [PubMed] [Google Scholar]
  7. NEWCOMER E. H., DONNELLY G. M. Leukocyte culture for chromosome studies in the domestic fowl. Stain Technol. 1963 Jan;38:54–56. [PubMed] [Google Scholar]
  8. Nisbet N. W., Simonsen M. Primary immune response in grafted cells. Dissociation between the proliferation of activity and the proliferation of cells. J Exp Med. 1967 Jun 1;125(6):967–981. doi: 10.1084/jem.125.6.967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Owen J. J. Karyotype studies on Gallus domesticus. Chromosoma. 1965 May 26;16(5):601–608. doi: 10.1007/BF00326975. [DOI] [PubMed] [Google Scholar]
  10. SOLOMON J. B. A sex difference in the splenomegaly syndrome in chick embryos injected with adult spleen cells or blood. Exp Cell Res. 1962 Oct;28:151–157. doi: 10.1016/0014-4827(62)90324-5. [DOI] [PubMed] [Google Scholar]