BINDING OF SOLUBLE IMMUNE COMPLEXES TO HUMAN LYMPHOBLASTOID CELLS : II. Use of Raji Cells to Detect Circulating Immune Complexes in Animal and Human Sera (original) (raw)

J Exp Med. 1974 Oct 31; 140(5): 1230–1244.

II. Use of Raji Cells to Detect Circulating Immune Complexes in Animal and Human Sera

From Scripps Clinic and Research Foundation, La Jolla, California 92037

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Abstract

Cells from a human lymphoblastoid cell line (Raji), with B-cell characteristics, and having receptors for human IgG Fc, C3b, and C3d, were used in an immunofluorescence test as in vitro detectors of immune complexes in animal and human sera. By this test, as little as 200–300 ng aggregated human gamma globulin or immune complexes per ml serum could be detected. The receptors for IgG Fc on the Raji cells were shown to be inefficient in detecting aggregated human gamma globulin and binding of aggregates to these receptors was inhibited by physiologic concentrations of 7S human IgG. Enhancement of aggregated human gamma globulin binding and binding of immune complexes formed in vitro to Raji cells was observed when the receptors for complement on these cells were used. By using the receptors for complement on Raji cells, circulating immune complexes were detected in rabbits with acute serum sickness, in mice with acute lymphocytic choriomeningitis virus infection, and in humans with immune complex type glomerulonephritis. The Raji cell test may be useful in detecting complement fixing immune complexes in different disease states, in monitoring circulating complexes in patients with immune complex diseases and in identifying the antigen(s) responsible for the induction of pathogenic immune complexes in humans and animals.

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Selected References

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Dixon FJ. Presidential address. Pathogenesis of immunologic disease. J Immunol. 1972 Aug;109(2):187–192. [PubMed] [Google Scholar]
Agnello V, Winchester RJ, Kunkel HG. Precipitin reactions of the C1q component of complement with aggregated gamma-globulin and immune complexes in gel diffusion. Immunology. 1970 Dec;19(6):909–919. [PMC free article] [PubMed] [Google Scholar]
Winchester RJ, Kunkel HG, Agnello V. Occurrence of -globulin complexes in serum and joint fluid of rheumatoid arthritis patients: use of monoclonal rheumatoid factors as reagents for their demonstration. J Exp Med. 1971 Sep 1;134(3 Pt 2):286s–295s. [PubMed] [Google Scholar]
Mowbray JF, Hoffbrand AV, Holborow EJ, Seah PP, Fry L. Circulating immune complexes in dermatitis herpetiformis. Lancet. 1973 Feb 24;1(7800):400–402. [PubMed] [Google Scholar]
Jewell DP, MacLennan IC. Circulating immune complexes in inflammatory bowel disease. Clin Exp Immunol. 1973 Jun;14(2):219–226. [PMC free article] [PubMed] [Google Scholar]
Creighton WD, Lambert PH, Miescher PA. Detection of antibodies and soluble antigen-antibody complexes by precipitation with polyethylene glycol. J Immunol. 1973 Oct;111(4):1219–1227. [PubMed] [Google Scholar]
FRANKLIN EC, HOLMAN HR, MULLER-EBERHARD HJ, KUNKEL HG. An unusual protein component of high molecular weight in the serum of certain patients with rheumatoid arthritis. J Exp Med. 1957 May 1;105(5):425–438. [PMC free article] [PubMed] [Google Scholar]
Dickler HB, Kunkel HG. Interaction of aggregated -globulin with B lymphocytes. J Exp Med. 1972 Jul 1;136(1):191–196. [PMC free article] [PubMed] [Google Scholar]
Bianco C, Patrick R, Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. [PMC free article] [PubMed] [Google Scholar]
Theofilopoulos AN, Dixon FJ, Bokisch VA. Binding of soluble immune complexes to human lymphoblastoid cells. I. Characterization of receptors for IgG Fc and complement and description of the binding mechanism. J Exp Med. 1974 Oct 1;140(4):877–894. [PMC free article] [PubMed] [Google Scholar]
PULVERTAFT JV. A STUDY OF MALIGNANT TUMOURS IN NIGERIA BY SHORT-TERM TISSUE CULTURE. J Clin Pathol. 1965 May;18:261–273. [PMC free article] [PubMed] [Google Scholar]
FERRARI A. Nitrogen determination by a continuous digestion and analysis system. Ann N Y Acad Sci. 1960 Jul 22;87:792–800. [PubMed] [Google Scholar]
Cuatrecasas P. Affinity chromatography. Annu Rev Biochem. 1971;40:259–278. [PubMed] [Google Scholar]
Clagett JA, Weigle WO. Roles of T and B lymphocytes in the termination of unresponsiveness to autologous thyroglobulin in mice. J Exp Med. 1974 Mar 1;139(3):643–660. [PMC free article] [PubMed] [Google Scholar]
Loor F, Forni L, Pernis B. The dynamic state of the lymphocyte membrane. Factors affecting the distribution and turnover of surface immunoglobulins. Eur J Immunol. 1972 Jun;2(3):203–212. [PubMed] [Google Scholar]
McConahey PJ, Dixon FJ. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. [PubMed] [Google Scholar]
CLARK HF, SHEPARD CC. A DIALYSIS TECHNIQUE FOR PREPARING FLUORESCENT ANTIBODY. Virology. 1963 Aug;20:642–644. [PubMed] [Google Scholar]
Wilson CB, Dixon FJ. Antigen quantitation in experimental immune complex glomerulonephritis. I. Acute serum sickness. J Immunol. 1970 Aug;105(2):279–290. [PubMed] [Google Scholar]
Oldstone MB, Dixon FJ. Pathogenesis of chronic disease associated with persistent lymphocytic choriomeningitis viral infection. I. Relationship of antibody production to disease in neonatally infected mice. J Exp Med. 1969 Mar 1;129(3):483–505. [PMC free article] [PubMed] [Google Scholar]
Wilson CB, Dixon FJ. Diagnosis of immunopathologic renal disease. Kidney Int. 1974 Jun;5(6):389–401. [PubMed] [Google Scholar]
CHRISTIAN CL. Studies of aggregated gamma-globulin. I. Sedimentation, electrophoretic and anticomplementary properties. J Immunol. 1960 Jan;84:112–116. [PubMed] [Google Scholar]
ISHIZAKA K. Gamma globulin and molecular mechanisms in hypersensitivity reactions. Prog Allergy. 1963;7:32–106. [PubMed] [Google Scholar]
Arend WP, Mannik M. In vitro adherence of soluble immune complexes to macrophages. J Exp Med. 1972 Sep 1;136(3):514–531. [PMC free article] [PubMed] [Google Scholar]
Dickler HB, Siegal FP, Bentwich ZH, Kunkel HG. Lymphocyte binding of aggregated IgG and surface Ig staining in chronic lymphocytic leukaemia. Clin Exp Immunol. 1973 May;14(1):97–106. [PMC free article] [PubMed] [Google Scholar]
Eden A, Bianco C, Nussenzweig Mechanism of binding of soluble immune complexes to lymphocytes. Cell Immunol. 1973 Jun;7(3):459–473. [PubMed] [Google Scholar]
Cruchaud A, Unanue ER. Fate and immunogenicity of antigens endocytosed by macrophages: a study using foreign red cells and immunoglobulin G. J Immunol. 1971 Nov;107(5):1329–1340. [PubMed] [Google Scholar]
Agnello V, Koffler D, Eisenberg JW, Winchester RJ, Kundel HG. C1g precipitins in the sera of patients with systemic lupus erythematosus and other hypocomplementemic states: characterization of high and low molecular weight types. J Exp Med. 1971 Sep 1;134(3 Pt 2):228s–241s. [PubMed] [Google Scholar]
Cochrane CG, Hawkins D. Studies on circulating immune complexes. 3. Factors governing the ability of circulating complexes to localize in blood vessels. J Exp Med. 1968 Jan 1;127(1):137–154. [PMC free article] [PubMed] [Google Scholar]
Kniker WT, Cochrane CG. The localization of circulating immune complexes in experimental serum sickness. The role of vasoactive amines and hydrodynamic forces. J Exp Med. 1968 Jan 1;127(1):119–136. [PMC free article] [PubMed] [Google Scholar]

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