The Effects of Intracellular Iontophoretic Injection of Calcium and Sodium Ions on the Light Response of Limulus Ventral Photoreceptors (original) (raw)

Abstract

During intracellular iontophoretic injection of Ca++ into Limulus ventral photoreceptor cells, there is a progressive diminution of the light response. Following Ca++ injection, the size of the light response slowly recovers. Similarly, there is a progressive diminution of the light response during intracellular injection of Na+ and recovery after the injection is stopped. The rate of diminution during Na+ injection is greater for higher [Ca++]out. In solutions which contain 0.1 mM Ca++, there is nearly no progressive decrease in the size of the light response during Na+ injection. Intracellular injections of Li+ or K+ do not progressively decrease the size of the light response. We propose that an increase in [Na+]in leads to an increase in [Ca++]in and that an increase in [Ca++]in by any means leads to a reduction in responsiveness to light.

Full Text

The Full Text of this article is available as a PDF (1.0 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown H. M., Hagiwara S., Koike H., Meech R. M. Membrane properties of a barnacle photoreceptor examined by the voltage clamp technique. J Physiol. 1970 Jun;208(2):385–413. doi: 10.1113/jphysiol.1970.sp009127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown J. E., Lisman J. E. An electrogenic sodium pump in Limulus ventral photoreceptor cells. J Gen Physiol. 1972 Jun;59(6):720–733. doi: 10.1085/jgp.59.6.720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark A. W., Millecchia R., Mauro A. The ventral photoreceptor cells of Limulus. I. The microanatomy. J Gen Physiol. 1969 Sep;54(3):289–309. doi: 10.1085/jgp.54.3.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FUORTES M. G. Electric activity of cells in the eye of Limulus. Am J Ophthalmol. 1958 Nov;46(5 Pt 2):210–223. doi: 10.1016/0002-9394(58)90800-6. [DOI] [PubMed] [Google Scholar]
  5. KIKUCHI R., NAITO K., TANAKA I. Effect of sodium and potassium ions on the electrical activity of single cells in the lateral eye of the horseshoe crab. J Physiol. 1962 May;161:319–343. doi: 10.1113/jphysiol.1962.sp006889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lisman J. E., Brown J. E. Two light-induced processes in the photoreceptor cells of Limulus ventral eye. J Gen Physiol. 1971 Nov;58(5):544–561. doi: 10.1085/jgp.58.5.544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Millecchia R., Mauro A. The ventral photoreceptor cells of Limulus. 3. A voltage-clamp study. J Gen Physiol. 1969 Sep;54(3):331–351. doi: 10.1085/jgp.54.3.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Millecchia R., Mauro A. The ventral photoreceptor cells of Limulus. II. The basic photoresponse. J Gen Physiol. 1969 Sep;54(3):310–330. doi: 10.1085/jgp.54.3.310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. TOMITA T. The nature of action potentials in the lateral eye of the horseshoe crab as revealed by simultaneous intra- and extracellular recording. Jpn J Physiol. 1956 Dec 31;6(4):327–340. doi: 10.2170/jjphysiol.6.327. [DOI] [PubMed] [Google Scholar]