Chemotactic behavior of Campylobacter jejuni (original) (raw)
Abstract
The chemotactic behavior of Campylobacter jejuni was determined in the presence of different amino acids, carbohydrates, organic acids, and preparations and constituents of mucin and bile. L-Fucose was the only carbohydrate and L-aspartate, L-cysteine, L-glutamate, and L-serine were the only amino acids producing a chemotactic (positive) response. Several salts of organic acids, including pyruvate, succinate, fumarate, citrate, malate, and alpha-ketoglutarate, were also chemoattractants, as were bile (beef, chicken, and oxgall) and mucin (bovine gallbladder and hog gastric). Most constituents of bile tested individually were chemorepellents, but the mucin component was chemoattractant. The chemotactic behavior of C. jejuni toward L-fucose, a constituent of both bile and mucin, may be an important factor in the affinity of the organism for the gallbladder and intestinal tract.

Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J Gen Microbiol. 1973 Jan;74(1):77–91. doi: 10.1099/00221287-74-1-77. [DOI] [PubMed] [Google Scholar]
- Allweiss B., Dostal J., Carey K. E., Edwards T. F., Freter R. The role of chemotaxis in the ecology of bacterial pathogens of mucosal surfaces. Nature. 1977 Mar 31;266(5601):448–450. doi: 10.1038/266448a0. [DOI] [PubMed] [Google Scholar]
- Blaser M. J., Hardesty H. L., Powers B., Wang W. L. Survival of Campylobacter fetus subsp. jejuni in biological milieus. J Clin Microbiol. 1980 Apr;11(4):309–313. doi: 10.1128/jcm.11.4.309-313.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blaser M. J., Wells J. G., Feldman R. A., Pollard R. A., Allen J. R. Campylobacter enteritis in the United States. A multicenter study. Ann Intern Med. 1983 Mar;98(3):360–365. doi: 10.7326/0003-4819-98-3-360. [DOI] [PubMed] [Google Scholar]
- Bryner J. H., O'Berry P. A., Estes P. C., Foley J. W. Studies of vibrios from gallbladder of market sheep and cattle. Am J Vet Res. 1972 Jul;33(7):1439–1444. [PubMed] [Google Scholar]
- Clamp J. R. Chemical aspects of mucus. General considerations. Br Med Bull. 1978 Jan;34(1):25–27. doi: 10.1093/oxfordjournals.bmb.a071455. [DOI] [PubMed] [Google Scholar]
- Creeth J. M., Denborough M. A. The use of equilibrium-density-gradient methods for the preparation and characterization of blood-group-specific glycoproteins. Biochem J. 1970 May;117(5):879–891. doi: 10.1042/bj1170879. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freter R., O'Brien P. C., Macsai M. S. Effect of chemotaxis on the interaction of cholera vibrios with intestinal mucosa. Am J Clin Nutr. 1979 Jan;32(1):128–132. doi: 10.1093/ajcn/32.1.128. [DOI] [PubMed] [Google Scholar]
- LaMarre A. G., Straley S. C., Conti S. F. Chemotaxis toward amino acids by Bdellovibrio bacteriovorus. J Bacteriol. 1977 Jul;131(1):201–207. doi: 10.1128/jb.131.1.201-207.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Larsen S. H., Reader R. W., Kort E. N., Tso W. W., Adler J. Change in direction of flagellar rotation is the basis of the chemotactic response in Escherichia coli. Nature. 1974 May 3;249(452):74–77. doi: 10.1038/249074a0. [DOI] [PubMed] [Google Scholar]
- Lee A., O'Rourke J. L., Barrington P. J., Trust T. J. Mucus colonization as a determinant of pathogenicity in intestinal infection by Campylobacter jejuni: a mouse cecal model. Infect Immun. 1986 Feb;51(2):536–546. doi: 10.1128/iai.51.2.536-546.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mantle M., Allen A. A colorimetric assay for glycoproteins based on the periodic acid/Schiff stain [proceedings]. Biochem Soc Trans. 1978;6(3):607–609. doi: 10.1042/bst0060607. [DOI] [PubMed] [Google Scholar]
- Mantle M., Allen A. Isolation and characterization of the native glycoprotein from pig small-intestinal mucus. Biochem J. 1981 Apr 1;195(1):267–275. doi: 10.1042/bj1950267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Merril C. R., Goldman D., Sedman S. A., Ebert M. H. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science. 1981 Mar 27;211(4489):1437–1438. doi: 10.1126/science.6162199. [DOI] [PubMed] [Google Scholar]
- Mesibov R., Adler J. Chemotaxis toward amino acids in Escherichia coli. J Bacteriol. 1972 Oct;112(1):315–326. doi: 10.1128/jb.112.1.315-326.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pearson J. P., Kaura R., Taylor W., Allen A. The composition and polymeric structure of mucus glycoprotein from human gallbladder bile. Biochim Biophys Acta. 1982 Sep 7;706(2):221–228. doi: 10.1016/0167-4838(82)90490-3. [DOI] [PubMed] [Google Scholar]
- Scawen M., Allen A. The action of proteolytic enzymes on the glycoprotein from pig gastric mucus. Biochem J. 1977 May 1;163(2):363–368. doi: 10.1042/bj1630363. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schrager J., Oates M. D., Rosbottom A. The isolation and partial characterization of the principal biliary glycoprotein. Digestion. 1972;6(6):338–355. doi: 10.1159/000197252. [DOI] [PubMed] [Google Scholar]
- Silverman M., Simon M. Flagellar rotation and the mechanism of bacterial motility. Nature. 1974 May 3;249(452):73–74. doi: 10.1038/249073a0. [DOI] [PubMed] [Google Scholar]
- Smibert R. M. Vibrio fetus var. intestinalis isolated from the intestinal content of birds. Am J Vet Res. 1969 Aug;30(8):1437–1442. [PubMed] [Google Scholar]
- Smith B. F., LaMont J. T. Hydrophobic binding properties of bovine gallbladder mucin. J Biol Chem. 1984 Oct 10;259(19):12170–12177. [PubMed] [Google Scholar]
- Tso W. W., Adler J. Negative chemotaxis in Escherichia coli. J Bacteriol. 1974 May;118(2):560–576. doi: 10.1128/jb.118.2.560-576.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]