Agglutinins and antibodies to Francisella tularensis outer membrane antigens in the early diagnosis of disease during an outbreak of tularemia (original) (raw)

Abstract

Tularemia was diagnosed in 57 patients during an outbreak in central Norway in 1984 and 1985. Clinical categories of the disease showed seasonal variations. A bacterial microagglutination test and an enzyme-linked immunosorbent assay (ELISA) with class-specific antibodies against Francisella tularensis outer membrane (OM) antigens were evaluated for the early diagnosis of tularemia. ELISA with immunoglobulin G (IgG), IgA, or IgM antibodies and the microagglutination test differed only marginally in diagnostic sensitivity. The OM preparation harbored F. tularensis agglutinogens and contained a variety of proteins, several of which functioned as immunogens in tularemia patients, as shown by Western blotting (immunoblotting). All 12 patients tested produced antibodies against a 43,000-molecular-weight OM protein. Individual variation was noted with regard to antibody response against other OM antigens. The OM is a suitable antigen preparation in ELISA for the diagnosis of tularemia and, presumably, contains antigens important in the immunobiology of tularemia.

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Selected References

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  1. Behan K. A., Klein G. C. Reduction of Brucella species and Francisella tularensis cross-reacting agglutinins by dithiothreitol. J Clin Microbiol. 1982 Oct;16(4):756–757. doi: 10.1128/jcm.16.4.756-757.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown S. L., McKinney F. T., Klein G. C., Jones W. L. Evaluation of a safranin-O-stained antigen microagglutination test for francisella tularensis antibodies. J Clin Microbiol. 1980 Feb;11(2):146–148. doi: 10.1128/jcm.11.2.146-148.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carlsson H. E., Lindberg A. A., Lindberg G., Hederstedt B., Karlsson K. A., Agell B. O. Enzyme-linked immunosorbent assay for immunological diagnosis of human tularemia. J Clin Microbiol. 1979 Nov;10(5):615–621. doi: 10.1128/jcm.10.5.615-621.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dahlstrand S., Ringertz O., Zetterberg B. Airborne tularemia in Sweden. Scand J Infect Dis. 1971;3(1):7–16. doi: 10.3109/inf.1971.3.issue-1.02. [DOI] [PubMed] [Google Scholar]
  5. Gabay J. E., Blake M., Niles W. D., Horwitz M. A. Purification of Legionella pneumophila major outer membrane protein and demonstration that it is a porin. J Bacteriol. 1985 Apr;162(1):85–91. doi: 10.1128/jb.162.1.85-91.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Haug R. H., Pearson A. D. Human infections with Francisella tularensis in Norway. Development of a serological screening test. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(2):273–280. doi: 10.1111/j.1699-0463.1972.tb00160.x. [DOI] [PubMed] [Google Scholar]
  7. Karkhanis Y. D., Zeltner J. Y., Jackson J. J., Carlo D. J. A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of Gram-negative bacteria. Anal Biochem. 1978 Apr;85(2):595–601. doi: 10.1016/0003-2697(78)90260-9. [DOI] [PubMed] [Google Scholar]
  8. Koskela P., Herva E. Immunity against Francisella tularensis in northern Finland. Scand J Infect Dis. 1982;14(3):195–199. doi: 10.3109/inf.1982.14.issue-3.07. [DOI] [PubMed] [Google Scholar]
  9. Koskela P., Salminen A. Humoral immunity against Francisella tularensis after natural infection. J Clin Microbiol. 1985 Dec;22(6):973–979. doi: 10.1128/jcm.22.6.973-979.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Mocca L. F., Frasch C. E. Sodium dodecyl sulfate-polyacrylamide gel typing system for characterization of Neisseria meningitidis isolates. J Clin Microbiol. 1982 Aug;16(2):240–244. doi: 10.1128/jcm.16.2.240-244.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosenbusch J. P. Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding. J Biol Chem. 1974 Dec 25;249(24):8019–8029. [PubMed] [Google Scholar]
  13. Rødahl E., Iversen O. J. Analysis of circulating immune complexes from patients with ankylosing spondylitis by gel electrophoresis and immunoblotting using antiserum against a psoriasis associated retrovirus-like particle. Ann Rheum Dis. 1986 Nov;45(11):892–898. doi: 10.1136/ard.45.11.892. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sandström G., Tärnvik A., Wolf-Watz H. Immunospecific T-lymphocyte stimulation by membrane proteins from Francisella tularensis. J Clin Microbiol. 1987 Apr;25(4):641–644. doi: 10.1128/jcm.25.4.641-644.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sandström G., Tärnvik A., Wolf-Watz H., Löfgren S. Antigen from Francisella tularensis: nonidentity between determinants participating in cell-mediated and humoral reactions. Infect Immun. 1984 Jul;45(1):101–106. doi: 10.1128/iai.45.1.101-106.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Syrjälä H., Herva E., Ilonen J., Saukkonen K., Salminen A. A whole-blood lymphocyte stimulation test for the diagnosis of human tularemia. J Infect Dis. 1984 Dec;150(6):912–915. doi: 10.1093/infdis/150.6.912. [DOI] [PubMed] [Google Scholar]
  17. Syrjälä H., Koskela P., Ripatti T., Salminen A., Herva E. Agglutination and ELISA methods in the diagnosis of tularemia in different clinical forms and severities of the disease. J Infect Dis. 1986 Jan;153(1):142–145. doi: 10.1093/infdis/153.1.142. [DOI] [PubMed] [Google Scholar]
  18. Viljanen M. K., Nurmi T., Salminen A. Enzyme-linked immunosorbent assay (ELISA) with bacterial sonicate antigen for IgM, IgA, and IgG antibodies to Francisella tularensis: comparison with bacterial agglutination test and ELISA with lipopolysaccharide antigen. J Infect Dis. 1983 Oct;148(4):715–720. doi: 10.1093/infdis/148.4.715. [DOI] [PubMed] [Google Scholar]