Properties and Location of Poly(A) in Rous Sarcoma Virus RNA (original) (raw)

Abstract

The poly(A) sequence of 30 to 40_S_ Rous sarcoma virus RNA, prepared by digestion of the RNA with RNase T1, showed a rather homogenous electrophoretic distribution in formamide-polyacrylamide gels. Its size was estimated to be about 200 AMP residues. The poly(A) appears to be located at or near the 3′ end of the 30 to 40_S_ RNA because: (i) it contained one adenosine per 180 AMP residues, and because (ii) incubation of 30 to 40_S_ RNA with bacterial RNase H in the presence of poly(dT) removed its poly(A) without significantly affecting its hydrodynamic or electrophoretic properties in denaturing solvents. The viral 60 to 70_S_ RNA complex was found to consist of 30 to 40_S_ subunits both with (65%) and without (approximately 30%) poly(A). The heteropolymeric sequences of these two species of 30 to 40_S_ subunits have the same RNase T1-resistant oligonucleotide composition. Some, perhaps all, RNase T1-resistant oligonucleotides of 30 to 40_S_ Rous sarcoma virus RNA appear to have a unique location relative to the poly(A) sequence, because the complexity of poly(A)-tagged fragments of 30 to 40_S_ RNA decreased with decreasing size of the fragment. Two RNase T1-resistant oligonucleotides which distinguish sarcoma virus Prague B RNA from that of a transformation-defective deletion mutant of the same virus appear to be associated with an 11_S_ poly(A)-tagged fragment of Prague B RNA. Thus RNA sequences concerned with cell transformation seem to be located within 5 to 10% of the 3′ terminus of Prague B RNA.

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