Mumps Virus Replication in Chick Embryo Lung Cells: Properties of Ribonucleic Acids in Virions and Infected Cells (original) (raw)

J Virol. 1971 Aug; 8(2): 161–173.

aLaboratories of Virology and Immunology, St. Jude Children's Research Hospital, and University of Tennessee Medical Units, Memphis, Tennessee 38101

2 Present address: Department of Virology, The University of Texas M. D. Anderson Hospital & Tumor Institute, Houston, Tex. 77025.

1 Part of this work was taken from a dissertation by J.L.E. in partial fulfillment of the requirements for the Ph.D. degree from the University of Tennessee, Memphis.

Abstract

Ribonucleic acid (RNA) species in mumps virions and in infected cells were compared. The predominant RNA species in virions labeled with 3H-uridine sedimented at 50_S_; RNA species sedimenting at 28, 18, and about 10_S_ were also present. The virion-associated RNA species sedimenting slower than 50_S_ contained some nucleotide sequences similar to 50_S_ virion RNA. Although mumps virus replication was severely inhibited by high concentrations of actinomycin D, some virus was made, and virus-specific RNA species accumulated in infected cells. Mumps virus resembled other paramyxoviruses in inducing, in infected cells, synthesis not only of 50_S_ RNA but also of slower sedimenting RNA species with a peak distribution at about 18_S_, complementary in base sequences to 50_S_ virion RNA. In addition, base sequences of the parental type were relatively abundant in the RNA species sedimenting slower than 50_S_; these may represent precursors of the slowly sedimenting RNA species associated with virions. Ribonuclease-resistant RNA was detected in infected cells; this may represent replicative or transcriptive intermediates. Inhibition of protein synthesis with cycloheximide severely depressed accumulation of labeled 50_S_ RNA in infected cells but did not interfere with accumulation of RNA species sedimenting slower than 50_S_. Actinomycin D treatment had a similar effect. Annealing of genomes and virus-induced complementary RNA species of Newcastle disease virus, Sendai virus, and mumps virus did not reveal any base sequence homologies.

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Selected References

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Blair CD, Duesberg PH. Myxovirus ribonucleic acids. Annu Rev Microbiol. 1970;24:539–574. [PubMed] [Google Scholar]
Blair CD, Robinson WS. Replication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology. 1968 Aug;35(4):537–549. [PubMed] [Google Scholar]
Bratt MA, Robinson WS. Evidence for an RNA replicative intermediate in cells infected with Newcastle disease virus. J Gen Virol. 1971 Feb;10(2):139–145. [PubMed] [Google Scholar]
Buynak EB, Hilleman MR. Live attenuated mumps virus vaccine. 1. Vaccine development. Proc Soc Exp Biol Med. 1966 Dec;123(3):768–775. [PubMed] [Google Scholar]
CANTELL K. Mumps virus. Adv Virus Res. 1961;8:123–164. [PubMed] [Google Scholar]
Choppin PW. Replication of influenza virus in a continuous cell line: high yield of infective virus from cells inoculated at high multiplicity. Virology. 1969 Sep;39(1):130–134. [PubMed] [Google Scholar]
Compans RW, Choppin PW. The length of the helical nucleocapsid of Newcastle disease virus. Virology. 1967 Oct;33(2):344–346. [PubMed] [Google Scholar]
Darlington RW, Portner A, Kingsbury DW. Sendai virus replication: an ultrastructural comparison of productive and abortive infections in avian cells. J Gen Virol. 1970 Dec;9(3):169–177. [PubMed] [Google Scholar]
FROTHINGHAM TE, GRANOFF A. Plaque formation with mumps virus. Virology. 1961 Oct;15:213–214. [PubMed] [Google Scholar]
Hosaka Y, Shimizu K. Lengths of the nucleocapsids of Newcastle disease and mumps viruses. J Mol Biol. 1968 Jul 28;35(2):369–373. [PubMed] [Google Scholar]
Huang AS, Baltimore D, Bratt MA. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. [PMC free article] [PubMed] [Google Scholar]
Kingsbury DW. Newcastle disease virus RNA. I. Isolation and preliminary characterization of RNA from virus particles. J Mol Biol. 1966 Jun;18(1):195–203. [PubMed] [Google Scholar]
Kingsbury DW. Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. J Mol Biol. 1966 Jun;18(1):204–214. [PubMed] [Google Scholar]
Kingsbury DW. Newcastle disease virus complementary RNA: its relationship to the viral genome and its accumulation in the presence or absence of actinomycin D. Virology. 1967 Oct;33(2):227–233. [PubMed] [Google Scholar]
Kingsbury DW. Replication and functions of myxovirus ribonucleic acids. Prog Med Virol. 1970;12:49–77. [PubMed] [Google Scholar]
Kingsbury DW, Granoff A. Studies on mixed infection with Newcastle disease virus. IV. On the structure of heterozygotes. Virology. 1970 Sep;42(1):262–265. [PubMed] [Google Scholar]
Kingsbury DW, Portner A, Darlington RW. Properties of incomplete Sendai virions and subgenomic viral RNAs. Virology. 1970 Dec;42(4):857–871. [PubMed] [Google Scholar]
Matumoto M. Multiplication of measles virus in cell cultures. Bacteriol Rev. 1966 Mar;30(1):152–176. [PMC free article] [PubMed] [Google Scholar]
Mountcastle WE, Compans RW, Choppin PW. Proteins and glycoproteins of paramyxoviruses: a comparison of simian virus 5, Newcastle disease virus, and Sendai virus. J Virol. 1971 Jan;7(1):47–52. [PMC free article] [PubMed] [Google Scholar]
Northrop RL. Effect of puromycin and actinomycin D on a persistent mumps virus infection in vitro. J Virol. 1969 Aug;4(2):133–140. [PMC free article] [PubMed] [Google Scholar]
Portner A, Kingsbury DW. Complementary RNAs in paramyxovirions and paramyxovirus-infected cells. Nature. 1970 Dec 19;228(5277):1196–1197. [PubMed] [Google Scholar]
Robinson WS. Self-annealing of subgroup 2 myxovirus RNAs. Nature. 1970 Mar 7;225(5236):944–945. [PubMed] [Google Scholar]
Rott R, Scholtissek C. Multiplication of myxoviruses. Mod Trends Med Virol. 1967;1(0):25–48. [PubMed] [Google Scholar]
VONHIPPEL PH, FELSENFELD G. MICROCOCCAL NUCLEASE AS A PROBE OF DNA CONFORMATION. Biochemistry. 1964 Jan;3:27–39. [PubMed] [Google Scholar]
Zhdanov VM, Kingsberi DU. Replikativnaia promezhutochnaia forma RNK v kletkakh, zarazhennykh virusom bolozni N'iukasla VB. Vopr Virusol. 1969 Sep-Oct;14(5):537–541. [PubMed] [Google Scholar]

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