Genes that modify expression of major urinary proteins in mice (original) (raw)

Abstract

A survey of major urinary proteins (MUPs) from eight BALB/c mouse substrains by isoelectric focusing identified a common pattern with about 10 protein bands in males. One substrain, BALB/cJPt, differed in that it expressed two variant MUP patterns, designated 4.1lo and null. To find the chromosomal location of the gene which determines the 4.1lo phenotype, BALB/cJPt-MUP-4.1lo was crossed with a wild-derived Mus musculus domesticus inbred strain (CLA) that expresses the common BALB/c MUP pattern. The F1 phenotype revealed that the gene(s) controlling the MUP-4.1lo trait was recessive. A restriction fragment polymorphism between these strains found with a MUP cDNA probe allowed us to establish that a gene determining the MUP-4.1lo trait was not linked to the MUP structural genes on chromosome 4. Assays for other chromosomal marker loci revealed that a gene determining the MUP-4.1lo trait, designated Mupm-1, was closely linked to Myc-1 on chromosome 15. To determine the genetic basis of the null trait, BALB/cJPt-MUP-null mice were crossed with BALB/cJPt-MUP-4.1lo mice. A MUP restriction fragment polymorphism between these two lines was tightly linked to a gene or genes involved in determining the MUP-null phenotype. The two variant MUP phenotypes in BALB/cJ mice are determined by separate genes, one of which is located on chromosome 4 and the other on chromosome 15. The chromosomal location of Mupm-1 suggests that it produces a trans-acting factor which regulates MUP expression.

2705

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Parker B. A., Reiser J., Renart J., Stark G. R., Wahl G. M. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol. 1979;68:220–242. doi: 10.1016/0076-6879(79)68017-5. [DOI] [PubMed] [Google Scholar]
  2. Bennett K. L., Lalley P. A., Barth R. K., Hastie N. D. Mapping the structural genes coding for the major urinary proteins in the mouse: combined use of recombinant inbred strains and somatic cell hybrids. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1220–1224. doi: 10.1073/pnas.79.4.1220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bishop J. O., Clark A. J., Clissold P. M., Hainey S., Francke U. Two main groups of mouse major urinary protein genes, both largely located on chromosome 4. EMBO J. 1982;1(5):615–620. doi: 10.1002/j.1460-2075.1982.tb01217.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ciaranello R. D., Hoffman H. J., Shire J. G., Axelrod J. Genetic regulation of the catecholamine biosynthetic enzymes. II. Inheritance of tyrosine hydroxylase, dopamine-beta-hydroxylase, and phenylethanolamine N-methyltransferase. J Biol Chem. 1974 Jul 25;249(14):4528–4536. [PubMed] [Google Scholar]
  5. Clark A. J., Chave-Cox A., Ma X., Bishop J. O. Analysis of mouse major urinary protein genes: variation between the exonic sequences of group 1 genes and a comparison with an active gene out with group 1 both suggest that gene conversion has occurred between MUP genes. EMBO J. 1985 Dec 1;4(12):3167–3171. doi: 10.1002/j.1460-2075.1985.tb04060.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clark A. J., Hickman J., Bishop J. A 45-kb DNA domain with two divergently orientated genes is the unit of organisation of the murine major urinary protein genes. EMBO J. 1984 Sep;3(9):2055–2064. doi: 10.1002/j.1460-2075.1984.tb02091.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. D'Hoostelaere L. A., Potter M. Igk polymorphism in M. musculus domesticus populations from Maryland and Delaware. Curr Top Microbiol Immunol. 1986;127:175–185. doi: 10.1007/978-3-642-71304-0_21. [DOI] [PubMed] [Google Scholar]
  8. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  9. FINLAYSON J. S., POTTER M., RUNNER C. R. ELECTROPHORETIC VARIATION AND SEX DIMORPHISM OF THE MAJOR URINARY PROTEIN COMPLEX IN INBRED MICE: A NEW GENETIC MARKER. J Natl Cancer Inst. 1963 Jul;31:91–107. [PubMed] [Google Scholar]
  10. Finlayson J. S., Asofsky R., Potter M., Runner C. C. Major urinary protein complex of normal mice: origin. Science. 1965 Aug 27;149(3687):981–982. doi: 10.1126/science.149.3687.981. [DOI] [PubMed] [Google Scholar]
  11. Hainey S., Bishop J. O. Allelic variation at several different genetic loci determines the major urinary protein phenotype of inbred mouse strains. Genet Res. 1982 Feb;39(1):31–39. doi: 10.1017/s0016672300020723. [DOI] [PubMed] [Google Scholar]
  12. Hastie N. D., Held W. A., Toole J. J. Multiple genes coding for the androgen-regulated major urinary proteins of the mouse. Cell. 1979 Jun;17(2):449–457. doi: 10.1016/0092-8674(79)90171-5. [DOI] [PubMed] [Google Scholar]
  13. Hogarth P. M., McKenzie I. F., Sutton V. R., Curnow K. M., Lee B. K., Eicher E. M. Mapping of the mouse Ly-6, Xp-14, and Gdc-1 loci to chromosome 15. Immunogenetics. 1987;25(1):21–27. doi: 10.1007/BF00768829. [DOI] [PubMed] [Google Scholar]
  14. Hudson D. M., Finlayson J. S., Potter M. Linkage of one component of the major urinary protein complex of mice to the brown coat color locus. Genet Res. 1967 Oct;10(2):195–198. doi: 10.1017/s0016672300010922. [DOI] [PubMed] [Google Scholar]
  15. Huppi K., Duncan R., Potter M. Myc-1 is centromeric to the linkage group Ly-6--Sis--Gdc-1 on mouse chromosome 15. Immunogenetics. 1988;27(3):215–219. doi: 10.1007/BF00346589. [DOI] [PubMed] [Google Scholar]
  16. Huppi K., Jouvin-Marche E., Scott C., Potter M., Weigert M. Genetic polymorphism at the kappa chain locus in mice: comparisons of restriction enzyme hybridization fragments of variable and constant region genes. Immunogenetics. 1985;21(5):445–457. doi: 10.1007/BF00430928. [DOI] [PubMed] [Google Scholar]
  17. Knopf J. L., Gallagher J. F., Held W. A. Differential, multihormonal regulation of the mouse major urinary protein gene family in the liver. Mol Cell Biol. 1983 Dec;3(12):2232–2240. doi: 10.1128/mcb.3.12.2232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kozak L. P., Birkenmeier E. H. Mouse sn-glycerol-3-phosphate dehydrogenase: molecular cloning and genetic mapping of a cDNA sequence. Proc Natl Acad Sci U S A. 1983 May;80(10):3020–3024. doi: 10.1073/pnas.80.10.3020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kozak L. P. Genetic variation in catecholamine responsive metabolic pathways--a hypothesis for a common regulatory mechanism in BALB/c sublines. Curr Top Microbiol Immunol. 1985;122:66–70. doi: 10.1007/978-3-642-70740-7_10. [DOI] [PubMed] [Google Scholar]
  20. Kuhn N. J., Woodworth-Gutai M., Gross K. W., Held W. A. Subfamilies of the mouse major urinary protein (MUP) multi-gene family: sequence analysis of cDNA clones and differential regulation in the liver. Nucleic Acids Res. 1984 Aug 10;12(15):6073–6090. doi: 10.1093/nar/12.15.6073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. LeClair K. P., Rabin M., Nesbitt M. N., Pravtcheva D., Ruddle F. H., Palfree R. G., Bothwell A. Murine Ly-6 multigene family is located on chromosome 15. Proc Natl Acad Sci U S A. 1987 Mar;84(6):1638–1642. doi: 10.1073/pnas.84.6.1638. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Leiter E. H. Differential susceptibility of BALB/c sublines to diabetes induction by multi-dose streptozotocin treatment. Curr Top Microbiol Immunol. 1985;122:78–85. doi: 10.1007/978-3-642-70740-7_12. [DOI] [PubMed] [Google Scholar]
  23. Meruelo D., Rossomando A., Scandalis S., D'Eustachio P., Fournier R. E., Roop D. R., Saxe D., Blatt C., Nesbitt M. N. Assignment of the Ly-6--Ril-1--Sis--H-30--Pol-5/Xmmv-72--Ins-3--Krt-1--Int-1 --Gdc-1 region to mouse chromosome 15. Immunogenetics. 1987;25(6):361–372. doi: 10.1007/BF00396102. [DOI] [PubMed] [Google Scholar]
  24. Potter M. History of the BALB/c family. Curr Top Microbiol Immunol. 1985;122:1–5. doi: 10.1007/978-3-642-70740-7_1. [DOI] [PubMed] [Google Scholar]
  25. Potter M., Wax J. S. Genetics of susceptibility to pristane-induced plasmacytomas in BALB/cAn: reduced susceptibility in BALB/cJ with a brief description of pristane-induced arthritis. J Immunol. 1981 Oct;127(4):1591–1595. [PubMed] [Google Scholar]
  26. RUEMKE P., THUNG P. J. IMMUNOLOGICAL STUDIES ON THE SEX-DEPENDENT PREALBUMIN IN MOUSE URINE AND ON ITS OCCURRENCE IN THE SERUM. Acta Endocrinol (Copenh) 1964 Sep;47:156–164. doi: 10.1530/acta.0.0470156. [DOI] [PubMed] [Google Scholar]
  27. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  28. Roderick T. H., Langley S. H., Leiter E. H. Some unusual genetic characteristics of BALB/c and evidence for genetic variation among BALB/c substrains. Curr Top Microbiol Immunol. 1985;122:9–18. doi: 10.1007/978-3-642-70740-7_2. [DOI] [PubMed] [Google Scholar]
  29. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  30. Stanton L. W., Watt R., Marcu K. B. Translocation, breakage and truncated transcripts of c-myc oncogene in murine plasmacytomas. Nature. 1983 Jun 2;303(5916):401–406. doi: 10.1038/303401a0. [DOI] [PubMed] [Google Scholar]