Classification of methanogenic bacteria by 16S ribosomal RNA characterization (original) (raw)

Proc Natl Acad Sci U S A. 1977 Oct; 74(10): 4537–4541.

George E. Fox

*Department of Genetics and Development, University of Illinois, Urbana, Illinois 61801

†Department of Microbiology, University of Illinois, Urbana, Illinois 61801

Linda J. Magrum

*Department of Genetics and Development, University of Illinois, Urbana, Illinois 61801

Carl R. Woese

*Department of Genetics and Development, University of Illinois, Urbana, Illinois 61801

*Department of Genetics and Development, University of Illinois, Urbana, Illinois 61801

†Department of Microbiology, University of Illinois, Urbana, Illinois 61801

† Present address: Department of Biophysical Sciences, University of Houston, Houston, TX 77004.

Abstract

The 16S ribosomal RNAs from 10 species of methanogenic bacteria have been characterized in terms of the oligonucleotides produced by T1 RNase digestion. Comparative analysis of these data reveals the methanogens to constitute a distinct phylogenetic group containing two major divisions. These organisms appear to be only distantly related to typical bacteria.

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