Abundant expression of EBER1 small nuclear RNA in nasopharyngeal carcinoma. A morphologically distinctive target for detection of Epstein-Barr virus in formalin-fixed paraffin-embedded carcinoma specimens. (original) (raw)
- Journal List
- Am J Pathol
- v.138(6); 1991 Jun
- PMC1886387
Am J Pathol. 1991 Jun; 138(6): 1461–1469.
Abstract
The highly restricted expression of the Epstein-Barr virus (EBV) genome in malignancy has limited the use of EBV detection methods applicable to formalin-fixed paraffin-embedded carcinoma specimens. In EBV-transformed lymphocytes very short nonprotein coding EBV transcripts (EBERs) are expressed in much higher abundance (10(7) copies per cell) than other EBV latency transcripts. Using a 3H riboprobe, the authors demonstrated EBER1 expression in NASOPHARYNGEAL CARCINOMAS (NPCs) as well as in parotid salivary gland. Recognition of EBER1 expression was facilitated by the intensity of hybridization and its characteristic morphology (nuclear with nucleolar sparing). EBER1 expression was not demonstrated in other epithelial malignancies arising from mucosal surfaces (oropharynx, uterine cervix) from which EBV shedding has been detected. Repeat study of the NPC specimens with digoxigenin-labeled probe yielded hybridization signal with subcellular morphologic detail and without background in a 12-hour procedure. Thus the EBER1 transcript is an appropriate target for in situ hybridization detection of EBV in formalin-fixed paraffin-embedded carcinoma specimens.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (3.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Images in this article
Click on the image to see a larger version.
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Nonoyama M, Pagano JS. Homology between Epstein-Barr virus DNA and viral DNA from Burkitt's lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics. Nature. 1973 Mar 2;242(5392):44–47. [PubMed] [Google Scholar]
- Nonoyama M, Huang CH, Pagano JS, Klein G, Singh S. DNA of Epstein-Barr virus detected in tissue of Burkitt's lymphoma and nasopharyngeal carcinoma. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3265–3268. [PMC free article] [PubMed] [Google Scholar]
- Andersson-Anvret M, Forsby N, Klein G, Henle W. Relationship between the Epstein-Barr virus and undifferentiated nasopharyngeal carcinoma: correlated nucleic acid hybridization and histopathological examination. Int J Cancer. 1977 Oct 15;20(4):486–494. [PubMed] [Google Scholar]
- Andersson-Anvret M, Klein G, Forsby N, Henle W. The association between undifferentiated nasopharyngeal carcinoma and Epstein-Barr virus shown by correlated nucleic acid hybridization and histopathological studies. IARC Sci Publ. 1978;(20):347–357. [PubMed] [Google Scholar]
- Andersson-Anvret M, Forsby N, Klein G, Henle W, Biörklund A. Relationship between the Epstein-Barr virus genome and nasopharyngeal carcinoma in Caucasian patients. Int J Cancer. 1979 Jun 15;23(6):762–767. [PubMed] [Google Scholar]
- Raab-Traub N, Flynn K, Pearson G, Huang A, Levine P, Lanier A, Pagano J. The differentiated form of nasopharyngeal carcinoma contains Epstein-Barr virus DNA. Int J Cancer. 1987 Jan 15;39(1):25–29. [PubMed] [Google Scholar]
- Young LS, Dawson CW, Clark D, Rupani H, Busson P, Tursz T, Johnson A, Rickinson AB. Epstein-Barr virus gene expression in nasopharyngeal carcinoma. J Gen Virol. 1988 May;69(Pt 5):1051–1065. [PubMed] [Google Scholar]
- Lerner MR, Andrews NC, Miller G, Steitz JA. Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1981 Feb;78(2):805–809. [PMC free article] [PubMed] [Google Scholar]
- Arrand JR, Rymo L. Characterization of the major Epstein-Barr virus-specific RNA in Burkitt lymphoma-derived cells. J Virol. 1982 Feb;41(2):376–389. [PMC free article] [PubMed] [Google Scholar]
- Glickman JN, Howe JG, Steitz JA. Structural analyses of EBER1 and EBER2 ribonucleoprotein particles present in Epstein-Barr virus-infected cells. J Virol. 1988 Mar;62(3):902–911. [PMC free article] [PubMed] [Google Scholar]
- Baer R, Bankier AT, Biggin MD, Deininger PL, Farrell PJ, Gibson TJ, Hatfull G, Hudson GS, Satchwell SC, Séguin C, et al. DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature. 1984 Jul 19;310(5974):207–211. [PubMed] [Google Scholar]
- Wu TC, Mann RB, Charache P, Hayward SD, Staal S, Lambe BC, Ambinder RF. Detection of EBV gene expression in Reed-Sternberg cells of Hodgkin's disease. Int J Cancer. 1990 Nov 15;46(5):801–804. [PubMed] [Google Scholar]
- Wu TC, Mounts P. Sensitive detection of nucleic acids and protein of human papillomavirus type 6 in respiratory and genital tract papillomata. J Virol Methods. 1989 Jul;25(1):31–47. [PubMed] [Google Scholar]
- Howe JG, Steitz JA. Localization of Epstein-Barr virus-encoded small RNAs by in situ hybridization. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9006–9010. [PMC free article] [PubMed] [Google Scholar]
- Lemon SM, Hutt LM, Shaw JE, Li JL, Pagano JS. Replication of EBV in epithelial cells during infectious mononucleosis. Nature. 1977 Jul 21;268(5617):268–270. [PubMed] [Google Scholar]
- Sixbey JW, Nedrud JG, Raab-Traub N, Hanes RA, Pagano JS. Epstein-Barr virus replication in oropharyngeal epithelial cells. N Engl J Med. 1984 May 10;310(19):1225–1230. [PubMed] [Google Scholar]
- Sixbey JW, Lemon SM, Pagano JS. A second site for Epstein-Barr virus shedding: the uterine cervix. Lancet. 1986 Nov 15;2(8516):1122–1124. [PubMed] [Google Scholar]
- Weiss LM, Movahed LA, Butler AE, Swanson SA, Frierson HF, Jr, Cooper PH, Colby TV, Mills SE. Analysis of lymphoepithelioma and lymphoepithelioma-like carcinomas for Epstein-Barr viral genomes by in situ hybridization. Am J Surg Pathol. 1989 Aug;13(8):625–631. [PubMed] [Google Scholar]
- Lung ML, Chan KH, Lam WP, Kou SK, Choy D, Chan CW, Ng MH. In situ detection of Epstein-Barr virus markers in nasopharyngeal carcinoma patients. Oncology. 1989;46(5):310–317. [PubMed] [Google Scholar]
- Permeen AM, Sam CK, Pathmanathan R, Prasad U, Wolf H. Detection of Epstein-Barr virus DNA in nasopharyngeal carcinoma using a non-radioactive digoxigenin-labelled probe. J Virol Methods. 1990 Mar;27(3):261–267. [PubMed] [Google Scholar]
- zur Hausen H, Schulte-Holthausen H, Klein G, Henle W, Henle G, Clifford P, Santesson L. EBV DNA in biopsies of Burkitt tumours and anaplastic carcinomas of the nasopharynx. Nature. 1970 Dec 12;228(5276):1056–1058. [PubMed] [Google Scholar]
- Wolf H, zur Hausen H, Becker V. EB viral genomes in epithelial nasopharyngeal carcinoma cells. Nat New Biol. 1973 Aug 22;244(138):245–247. [PubMed] [Google Scholar]
- Weiss LM, Movahed LA, Warnke RA, Sklar J. Detection of Epstein-Barr viral genomes in Reed-Sternberg cells of Hodgkin's disease. N Engl J Med. 1989 Feb 23;320(8):502–506. [PubMed] [Google Scholar]
- Weiss LM, Movahed LA. In situ demonstration of Epstein-Barr viral genomes in viral-associated B cell lymphoproliferations. Am J Pathol. 1989 Mar;134(3):651–659. [PMC free article] [PubMed] [Google Scholar]
- Bashir RM, Harris NL, Hochberg FH, Singer RM. Detection of Epstein-Barr virus in CNS lymphomas by in-situ hybridization. Neurology. 1989 Jun;39(6):813–817. [PubMed] [Google Scholar]
- Anagnostopoulos I, Herbst H, Niedobitek G, Stein H. Demonstration of monoclonal EBV genomes in Hodgkin's disease and Ki-1-positive anaplastic large cell lymphoma by combined Southern blot and in situ hybridization. Blood. 1989 Aug 1;74(2):810–816. [PubMed] [Google Scholar]
- Lawrence JB, Villnave CA, Singer RH. Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line. Cell. 1988 Jan 15;52(1):51–61. [PubMed] [Google Scholar]
- Teo CG, Griffin BE. Visualization of single copies of the Epstein-Barr virus genome by in situ hybridization. Anal Biochem. 1990 Apr;186(1):78–85. [PubMed] [Google Scholar]
- Young LS, Sixbey JW. Epstein-Barr virus and epithelial cells: a possible role for the virus in the development of cervical carcinoma. Cancer Surv. 1988;7(3):507–518. [PubMed] [Google Scholar]
- Weiland LH. Non-lymphoid tissue lesions induced by or associated with herpesvirus. J Virol Methods. 1988 Sep;21(1-4):73–78. [PubMed] [Google Scholar]
- Gilligan K, Rajadurai P, Resnick L, Raab-Traub N. Epstein-Barr virus small nuclear RNAs are not expressed in permissively infected cells in AIDS-associated leukoplakia. Proc Natl Acad Sci U S A. 1990 Nov;87(22):8790–8794. [PMC free article] [PubMed] [Google Scholar]
- Tugwood JD, Lau WH, O SK, Tsao SY, Martin WM, Shiu W, Desgranges C, Jones PH, Arrand JR. Epstein-Barr virus-specific transcription in normal and malignant nasopharyngeal biopsies and in lymphocytes from healthy donors and infectious mononucleosis patients. J Gen Virol. 1987 Apr;68(Pt 4):1081–1091. [PubMed] [Google Scholar]
- Katz BZ, Raab-Traub N, Miller G. Latent and replicating forms of Epstein-Barr virus DNA in lymphomas and lymphoproliferative diseases. J Infect Dis. 1989 Oct;160(4):589–598. [PubMed] [Google Scholar]
- Neel HB, 3rd, Pearson GR, Weiland LH, Taylor WF, Goepfert HH. Immunologic detection of occult primary cancer of the head and neck. Otolaryngol Head Neck Surg. 1981 Mar-Apr;89(2):230–234. [PubMed] [Google Scholar]
- Graeber GM, Reardon MJ, Fleming AW, Head HD, Zajtchuk R, Brott WH, Foster JH. An analysis of the isoenzymes of creatine phosphokinase and lactic dehydrogenase in the esophagus. Ann Thorac Surg. 1981 Sep;32(3):230–234. [PubMed] [Google Scholar]
- Halprin J, Scott AL, Jacobson L, Levine PH, Ho JH, Niederman JC, Hayward SD, Milman G. Enzyme-linked immunosorbent assay of antibodies to Epstein-Barr virus nuclear and early antigens in patients with infectious mononucleosis and nasopharyngeal carcinoma. Ann Intern Med. 1986 Mar;104(3):331–337. [PubMed] [Google Scholar]
- Leyvraz S, Henle W, Chahinian AP, Perlmann C, Klein G, Gordon RE, Rosenblum M, Holland JF. Association of Epstein-Barr virus with thymic carcinoma. N Engl J Med. 1985 May 16;312(20):1296–1299. [PubMed] [Google Scholar]
Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology