The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein (original) (raw)

Abstract

A 75,000-dalton protein has been purified approximately 1,000-fold from rat liver, based on its capacity to organize poly(A) in a 27-residue repeating structure. This protein may be identified with the major polypeptide component of cytoplasmic poly(A)-ribonucleoprotein (RNP) previously described. The poly(A)-organizing activity of the protein is detected only in cytoplasmic fractions. Upon nuclease digestion of the 75,000-dalton protein-poly(A) complex, monomers, and higher multimers of RNP subunits can be resolved in a sucrose gradient. The sedimentation rate of the monomer is compatible with a composition of one 75,000-dalton protein molecule and one 27-residue segment of poly(A).

Full Text

The Full Text of this article is available as a PDF (871.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baer B. W., Kornberg R. D. Repeating structure of cytoplasmic poly(A)-ribonucleoprotein. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1890–1892. doi: 10.1073/pnas.77.4.1890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barrieux A., Ingraham H. A., Nystul S., Rosenfeld M. G. Characterization of the association of specific proteins with messenger ribonucleic acid. Biochemistry. 1976 Aug 10;15(16):3523–3528. doi: 10.1021/bi00661a020. [DOI] [PubMed] [Google Scholar]
  3. Finch J. T., Noll M., Kornberg R. D. Electron microscopy of defined lengths of chromatin. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3320–3322. doi: 10.1073/pnas.72.9.3320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Firtel R. A., Pederson T. Ribonucleoprotein particles containing heterogeneous nuclear RNA in the cellular slime mold Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1975 Jan;72(1):301–305. doi: 10.1073/pnas.72.1.301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fukami H., Itano H. A. In vitro translation of globin: effect of proteins purified by affinity chromatography on polyadenylate-Sepharose. Biochemistry. 1976 Aug 10;15(16):3529–3535. doi: 10.1021/bi00661a021. [DOI] [PubMed] [Google Scholar]
  6. Kish V. M., Pederson T. Poly (A)-rich ribonucleoprotein complexes from HeLa cell messenger RNA. J Biol Chem. 1976 Oct 10;251(19):5888–5894. [PubMed] [Google Scholar]
  7. Kish V. M., Pederson T. Ribonucleoprotein organization of polyadenylate sequences in HeLa cell heterogeneous nuclear RNA. J Mol Biol. 1975 Jun 25;95(2):227–238. doi: 10.1016/0022-2836(75)90392-7. [DOI] [PubMed] [Google Scholar]
  8. Kwan S. W., Brawerman G. A particle associated with the polyadenylate segment in mammalian messenger RNA. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3247–3250. doi: 10.1073/pnas.69.11.3247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Laskey R. A., Honda B. M., Mills A. D., Finch J. T. Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA. Nature. 1978 Oct 5;275(5679):416–420. doi: 10.1038/275416a0. [DOI] [PubMed] [Google Scholar]
  11. Lindberg U., Sundquist B. Isolation of messenger ribonucleoproteins from mammalian cells. J Mol Biol. 1974 Jun 25;86(2):451–468. doi: 10.1016/0022-2836(74)90030-8. [DOI] [PubMed] [Google Scholar]
  12. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
  13. Morel C., Gander E. S., Herzberg M., Dubochet J., Scherrer K. The duck-globin messenger-ribonucleoprotein complex. Resistance to high ionic strength, particle gel electrophoresis, composition and visualisation by dark-field electron microscopy. Eur J Biochem. 1973 Jul 16;36(2):455–464. doi: 10.1111/j.1432-1033.1973.tb02930.x. [DOI] [PubMed] [Google Scholar]
  14. Quinlan T. J., Billings P. B., Martin T. E. Nuclear ribonucleoprotein complexes containing polyadenylate from mouse ascites cells. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2632–2636. doi: 10.1073/pnas.71.7.2632. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schwartz H., Darnell J. E. The association of protein with the polyadenylic acid of HeLa cell messenger RNA: evidence for a "transport" role of a 75,000 molecular weight polypeptide. J Mol Biol. 1976 Jul 15;104(4):833–851. doi: 10.1016/0022-2836(76)90185-6. [DOI] [PubMed] [Google Scholar]
  16. Schweiger A., Mazur G. Rat liver soluble nuclear and cytoplasmic proteins with high affinity to polynucleotides. FEBS Lett. 1975 Dec 1;60(1):114–118. doi: 10.1016/0014-5793(75)80430-3. [DOI] [PubMed] [Google Scholar]