Serial Observations on Patterns of Growth, Myelin Formation, Maintenance and Degeneration in Cultures of New-Born Rat and Kitten Cerebellum (original) (raw)
J Biophys Biochem Cytol. 1958 Sep 25; 4(5): 499–504.
From the Laboratory for Cell Physiology, Department of Surgery, College of Physicians and Surgeons, Columbia University, New York
Copyright © Copyright, 1958, by The Rockefeller Institute
Abstract
New-born rat and kitten cerebellum may be maintained for prolonged periods (over 5 months) in the Maximow assembly if explanted on to a coverslip previously coated with a thin gel of reconstituted rat tail collagen and fed a glucose-enriched "natural" medium. After a 2 week period of adjustment and early outgrowth, most cultures exhibit myelin formation. Axons located within the surrounding neuroglial sheet of the explant area myelinate. The sheaths are first evident as long, unsegmented, smooth, parallel, refractile lines. Simultaneously, neuronal nuclei tend to assume central positions and powdery granules of Nissl substance and lipoid materials begin to accumulate within the cytoplasm. During prolonged maintenance, axons may increase in width and the myelin may thicken. Some exhibit transient irregularities and swellings. Degeneration of some axons occurs manifested either by (a) progressive swellings and distortions of the myelin sheath and thinning of intervening portions of the axons which finally yield, leaving the swellings as myelin bodies, or by (b) small aneurysm-like distortions of myelin sheaths on thinning axons which become dull, irregular, and thread-like filaments beaded by the former herniations. The observations are compared with previous studies of in vitro and in vivo myelin formation with particular reference to neuronal-neuroglial relationships.
Full Text
The Full Text of this article is available as a PDF (1.3M).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- PETERSON ER, MURRAY MR. Myelin sheath formation in cultures of avian spinal ganglia. Am J Anat. 1955 May;96(3):319–355. [PubMed] [Google Scholar]
- COSTERO I, POMERAT CM. Tissue cultures of cat cerebellum. Am J Anat. 1956 Sep;99(2):211–247. [PubMed] [Google Scholar]
- HILD W. Myelogenesis in cultures of mammalian central nervous tissue. Z Zellforsch Mikrosk Anat. 1957;46(1):71–95. [PubMed] [Google Scholar]
- BORNSTEIN MB. Reconstituted rattail collagen used as substrate for tissue cultures on coverslips in Maximow slides and roller tubes. Lab Invest. 1958 Mar-Apr;7(2):134–137. [PubMed] [Google Scholar]
- KLUVER H, BARRERA E. A method for the combined staining of cells and fibers in the nervous system. J Neuropathol Exp Neurol. 1953 Oct;12(4):400–403. [PubMed] [Google Scholar]
- PEARSE AGE. Copper phthalocyanins as phospholipid stains. J Pathol Bacteriol. 1955 Oct;70(2):554–557. [PubMed] [Google Scholar]
- HILD W. [Ependymal cells in tissue culture]. Z Zellforsch Mikrosk Anat. 1957;46(3):259–271. [PubMed] [Google Scholar]
- LUSE SA. Formation of myelin in the central nervous system of mice and rats, as studied with the electron microscope. J Biophys Biochem Cytol. 1956 Nov 25;2(6):777–784. [PMC free article] [PubMed] [Google Scholar]
Articles from The Journal of Biophysical and Biochemical Cytology are provided here courtesy of The Rockefeller University Press